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mmu_circAno6_337
Basic information of circular RNA
PanCircBase ID:
mmu_circAno6_337
mm39_Chr. Position ID:
chr15|95853771|95860162|+
Gene name:
Ano6
Transcript:
ENSMUST00000071874.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
16,17
Exon/intron offsets:
0,6185
Genomic length:
6391
Exon/intron sizes in nt:
1,31,206
Flanking exon/introns:
chr15:95847837-95853771|chr15:95860162-95863701
Splice length:
337
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
ATGGGTTATGAATCTAATTGGACGATATAAAAGAGTCTCGGGATCAGAAAAGATAACCCCACGATGGGAACAGGATTACCATCTGCAGCCCATGGGCAAGCTGGGATTGTTCTATGAGTATCTTGAAATGATTATTCAGTTTGGGTTCGTCACTTTATTTGTGGCCTCTTTCCCACTGGCTCCCCTGCTGGCTCTGGTGAACAATATTTTGGAAATAAGAGTGGACGCGTGGAAGCTCACAACCCAGTTTAGGCGCATGGTGCCCGAAAAAGCCCAGGATATCGGCGCGTGGCAGCCCATCATGCAAGGAATAGCCATCCTGGCCGTGGTGACCAAC
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCCCGAAAAAGCCCAGGATA
60.4
CCATGGGCTGCAGATGGTAA
60.11
171
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GCCCGAAAAAGCCCAGGATA
60.4
TGGGCTGCAGATGGTAATCC
59.82
168
PCR template sequence:
CACAACCCAGTTTAGGCGCATGGTGCCCGAAAAAGCCCAGGATATCGGCGCGTGGCAGCCCATCATGCAAGGAATAGCCATCCTGGCCGTGGTGACCAACATGGGTTATGAATCTAATTGGACGATATAAAAGAGTCTCGGGATCAGAAAAGATAACCCCACGATGGGAACAGGATTACCATCTGCAGCCCATGGGCAAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-433-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CGTGGTGACCAACATGGGTTATGAAT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCANO6_337_ORF_1
1.014
0.203885198
0.514566066
360
ORF sequence:
ATGAATCTAATTGGACGATATAAAAGAGTCTCGGGATCAGAAAAGATAACCCCACGATGGGAACAGGATTACCATCTGCAGCCCATGGGCAAGCTGGGATTGTTCTATGAGTATCTTGAAATGATTATTCAGTTTGGGTTCGTCACTTTATTTGTGGCCTCTTTCCCACTGGCTCCCCTGCTGGCTCTGGTGAACAATATTTTGGAAATAAGAGTGGACGCGTGGAAGCTCACAACCCAGTTTAGGCGCATGGTGCCCGAAAAAGCCCAGGATATCGGCGCGTGGCAGCCCATCATGCAAGGAATAGCCATCCTGGCCGTGGTGACCAACATGGGTTATGAATCTAATTGGACGATATAA
Peptide sequence:
MNLIGRYKRVSGSEKITPRWEQDYHLQPMGKLGLFYEYLEMIIQFGFVTLFVASFPLAPLLALVNNILEIRVDAWKLTTQFRRMVPEKAQDIGAWQPIMQGIAILAVVTNMGYESNWTI
IRESfinder result
IRESfinder score
0.673573159
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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