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mmu_circAno10_1442
Basic information of circular RNA
PanCircBase ID:
mmu_circAno10_1442
mm39_Chr. Position ID:
chr9|122030836|122092855|-
Gene name:
Ano10
Transcript:
ENSMUST00000042546.4
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
8
Exon/intron indexes:
12,11,10,9,8,7,6,5
Exon/intron offsets:
0,49376,51165,55285,57146,57764,59313,61899
Genomic length:
62019
Exon/intron sizes in nt:
1,17,12,91,92,18,37,55,00,00,000
Flanking exon/introns:
chr9:122005544-122030836|chr9:122092855-122100264
Splice length:
1442
Other database IDs:
circAtlas: mmu-Ano10_0003
circAtlas: hsa-ANO10_0008, CIRCpedia: HSA_CIRCpedia_147923
Human Ortholog IDs:
Mature spliced Sequence:
TGAGGAGACTGCTCACGTCTGGCATCGTGACCCAAGTGTTCCCGCTGCATGACACTGAAGCCCTGAAGAAGCTGGAGGACACGTGGTACACTCGGTTTGCTCTCAAGTACCAGCCCATAGACAGCATTCGTAGCTACTTTGGGGAGACAATTGCTCTGTACTTCGGGTTCCTGGAGTACTTCACCTTTGCCTTAATCCCCATGGCCATCATTGGACTGCCCTACTACCTGTTTGTGTGGGAAGACTACGACAAGTATGTCATCTTTGCCTCGTTCAACCTCATATGGTCCACAGTGATCCTGGAGGTGTGGAAGCGTGGCTGTGCCAACATGACCTATCGCTGGGGGACTCTGGTCATGAAGAGGCAGTTTGAGGAGCCCCGGCCAGGGTTTCATGGAGTCCTTGGCATCAATTCAGTCACGGGCCGGGAGGAGCCCCTCTACTCGAGCTACAAGAGACAACTGCGCATCTACCTAGTCTCTCTGCCATTTGTGTGCCTCTGCCTTTATTTCTCCCTTTATGTCATGATGATCTACTTTGACATGGAGGACTGGGCCTTGAGTCTCCACGAGGACAGTGGGTCTGAGTGGACCAGCCTTCTGCTGTATGTACCCAGCATTGTTTATGCCGTTGTGATTGAGATCATGAACCGCCTCTATCGATATGCTGCCGAGTTCCTAACATCTTGGGAGAATCACAGATTGGAATCTGCCTACCAGAATCATCTCGTTCTGAAAGTGTTGGTGTTCAACTTCCTGAATTGCTTCGCCTCACTCTTCTACATTGCCTTTGTCCTGAAGGACATGAAGCTTTTGCGCCAGAGCTTGGCCACACTCCTGATCACCTCCCAGATTCTGAACCAAGTCGTAGAATCTCTTCTTCCTTACTGGCTGCAGCGGAAGTACTGTGCGAGGGTAAAGCGGAAGGTTCAGGCATTAAAGTCTGAGGTCGACACAACCTTGTATGAGCAAGTCCTCCTGGAGAAGGAAATGGGAACGTACCTGGGAACCTTTGATGATTACCTGGAGTTGTTCCTGCAGTTTGGCTATGTGAGCCTTTTCTCTTGTGTTTACCCGCTAGCAGCTGCCTTTGCTGTGTTAAATAACTTCACTGAAGTCAACTCAGATGCCTTGAAAATGTGCAGGGTCTTCAAACGGCCATTTGCAGAACCTTCGGCCAGTATTGGCGTATGGCAGTTGGCTTTTGAAACGATGAGCGTTATATCCGTGGTCACTAACTGTGCTCTGATTGGAATGTCACCACAAGTGAATGCAGTCTTTCCAGAATCCAAAACAGACCTTGTTCTGATTGTGGTGGCCGTAGAGCATGCACTCTTGGCTCTGAAGTTCATACTCGCATTTGCCATCCCTGATAAACCACGGCACATCCAACAGAAACTGGCCAGATTGGAATTCGAATCTTTGGAGGCACTCAAGCAACAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCCCTGATAAACCACGGCAC
60.04
AACCGAGTGTACCACGTGTC
59.97
174
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCCCTGATAAACCACGGCAC
60.04
CACGTGTCCTCCAGCTTCTT
59.97
162
PCR template sequence:
GAAGTTCATACTCGCATTTGCCATCCCTGATAAACCACGGCACATCCAACAGAAACTGGCCAGATTGGAATTCGAATCTTTGGAGGCACTCAAGCAACAGTGAGGAGACTGCTCACGTCTGGCATCGTGACCCAAGTGTTCCCGCTGCATGACACTGAAGCCCTGAAGAAGCTGGAGGACACGTGGTACACTCGGTTTGC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
10
Interacting miRNAs (#binding sites)
mmu-miR-134-5p(1), mmu-miR-429-3p(1), mmu-miR-200c-3p(1), mmu-miR-122-5p(1), mmu-miR-503-5p(1), mmu-miR-96-5p(1), mmu-miR-541-5p(1), mmu-miR-680(1), mmu-miR-188-3p(1), mmu-miR-466b-3p(1)
siRNAs for circular RNA silencing
Junction sequence
ACTCAAGCAACAGTGAGGAGACTGCT
siRNA sets
TCTCCTCACTGTTGCTTGA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCANO10_1442_ORF_1
1.2535
0.285947972
0.999234349
1245
ORF sequence:
ATGGCCATCATTGGACTGCCCTACTACCTGTTTGTGTGGGAAGACTACGACAAGTATGTCATCTTTGCCTCGTTCAACCTCATATGGTCCACAGTGATCCTGGAGGTGTGGAAGCGTGGCTGTGCCAACATGACCTATCGCTGGGGGACTCTGGTCATGAAGAGGCAGTTTGAGGAGCCCCGGCCAGGGTTTCATGGAGTCCTTGGCATCAATTCAGTCACGGGCCGGGAGGAGCCCCTCTACTCGAGCTACAAGAGACAACTGCGCATCTACCTAGTCTCTCTGCCATTTGTGTGCCTCTGCCTTTATTTCTCCCTTTATGTCATGATGATCTACTTTGACATGGAGGACTGGGCCTTGAGTCTCCACGAGGACAGTGGGTCTGAGTGGACCAGCCTTCTGCTGTATGTACCCAGCATTGTTTATGCCGTTGTGATTGAGATCATGAACCGCCTCTATCGATATGCTGCCGAGTTCCTAACATCTTGGGAGAATCACAGATTGGAATCTGCCTACCAGAATCATCTCGTTCTGAAAGTGTTGGTGTTCAACTTCCTGAATTGCTTCGCCTCACTCTTCTACATTGCCTTTGTCCTGAAGGACATGAAGCTTTTGCGCCAGAGCTTGGCCACACTCCTGATCACCTCCCAGATTCTGAACCAAGTCGTAGAATCTCTTCTTCCTTACTGGCTGCAGCGGAAGTACTGTGCGAGGGTAAAGCGGAAGGTTCAGGCATTAAAGTCTGAGGTCGACACAACCTTGTATGAGCAAGTCCTCCTGGAGAAGGAAATGGGAACGTACCTGGGAACCTTTGATGATTACCTGGAGTTGTTCCTGCAGTTTGGCTATGTGAGCCTTTTCTCTTGTGTTTACCCGCTAGCAGCTGCCTTTGCTGTGTTAAATAACTTCACTGAAGTCAACTCAGATGCCTTGAAAATGTGCAGGGTCTTCAAACGGCCATTTGCAGAACCTTCGGCCAGTATTGGCGTATGGCAGTTGGCTTTTGAAACGATGAGCGTTATATCCGTGGTCACTAACTGTGCTCTGATTGGAATGTCACCACAAGTGAATGCAGTCTTTCCAGAATCCAAAACAGACCTTGTTCTGATTGTGGTGGCCGTAGAGCATGCACTCTTGGCTCTGAAGTTCATACTCGCATTTGCCATCCCTGATAAACCACGGCACATCCAACAGAAACTGGCCAGATTGGAATTCGAATCTTTGGAGGCACTCAAGCAACAGTGA
Peptide sequence:
MAIIGLPYYLFVWEDYDKYVIFASFNLIWSTVILEVWKRGCANMTYRWGTLVMKRQFEEPRPGFHGVLGINSVTGREEPLYSSYKRQLRIYLVSLPFVCLCLYFSLYVMMIYFDMEDWALSLHEDSGSEWTSLLLYVPSIVYAVVIEIMNRLYRYAAEFLTSWENHRLESAYQNHLVLKVLVFNFLNCFASLFYIAFVLKDMKLLRQSLATLLITSQILNQVVESLLPYWLQRKYCARVKRKVQALKSEVDTTLYEQVLLEKEMGTYLGTFDDYLELFLQFGYVSLFSCVYPLAAAFAVLNNFTEVNSDALKMCRVFKRPFAEPSASIGVWQLAFETMSVISVVTNCALIGMSPQVNAVFPESKTDLVLIVVAVEHALLALKFILAFAIPDKPRHIQQKLARLEFESLEALKQQ
IRESfinder result
IRESfinder score
0.649912383
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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