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mmu_circAnln_386
Basic information of circular RNA
PanCircBase ID:
mmu_circAnln_386
mm39_Chr. Position ID:
chr9|22287432|22287818|-
Gene name:
Anln
Transcript:
ENSMUST00000040912.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
1
Exon/intron indexes:
4
Exon/intron offsets:
0
Genomic length:
386
Exon/intron sizes in nt:
386
Flanking exon/introns:
chr9:22286436-22287432|chr9:22287818-22291345
Splice length:
386
Other database IDs:
CIRCpedia: MMU_CIRCpedia_13518, circAtlas: mmu-Anln_0010
Human Ortholog IDs:
Mature spliced Sequence:
ATGATGTATCAGAAAGTTCATACTTTGCACCAGTGCCAACTGAGGACAAGGCTGCCTCACCTTCTAAGCCACCCATTTCAAATGCCTCAGCTACTCCAGTTGGGAGAAGGGGCCGTCTGGCCAACCTTGCTGCAACGATTTGCTCCTGGGAAGATGATGTAAGCCACTCATCTGCAAAGCAAAATAGTGTGCAAGAACAGCCTGGTACCGCTTGTTTATCCAAATCTTCCTCTGCAAGTGGAGCATCTGCTAGCATCAATAGCAGCAGTGTTCAGCAGGAAGCTACATGCTGTTCCCCAAGGGACGGCAATGCCTCTGTCAGGAAAGACCCATCTTCAAATGCTGCCCATGGACCTTTGCTTAGCGCCTCAGTGTCCAGCTCTGTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCCATCTTCAAATGCTGCCC
59.54
GGCATTTGAAATGGGTGGCT
59.39
144
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CCCATCTTCAAATGCTGCCC
59.54
AGGCATTTGAAATGGGTGGC
59.39
145
PCR template sequence:
ATGCTGTTCCCCAAGGGACGGCAATGCCTCTGTCAGGAAAGACCCATCTTCAAATGCTGCCCATGGACCTTTGCTTAGCGCCTCAGTGTCCAGCTCTGTGATGATGTATCAGAAAGTTCATACTTTGCACCAGTGCCAACTGAGGACAAGGCTGCCTCACCTTCTAAGCCACCCATTTCAAATGCCTCAGCTACTCCAGT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
12
Interacting miRNAs (#binding sites)
mmu-miR-200a-3p(1), mmu-miR-141-3p(1), mmu-miR-200b-3p(1), mmu-miR-103-3p(1), mmu-miR-150-5p(1), mmu-miR-200c-3p(1), mmu-miR-19a-3p(1), mmu-miR-298-5p(1), mmu-miR-96-5p(1), mmu-miR-107-3p(1), mmu-miR-207(1), mmu-miR-296-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GTCCAGCTCTGTGATGATGTATCAGA
siRNA sets
ACATCATCACAGAGCTGGA, CATCATCACAGAGCTGGAC, TACATCATCACAGAGCTGG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCANLN_386_ORF_3
0.9729
-0.107530187
0.034600492
78
ORF sequence:
ATGCCTCTGTCAGGAAAGACCCATCTTCAAATGCTGCCCATGGACCTTTGCTTAGCGCCTCAGTGTCCAGCTCTGTGA
Peptide sequence:
MPLSGKTHLQMLPMDLCLAPQCPAL
IRESfinder result
IRESfinder score
0.415384329
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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