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mmu_circAnln_1367
Basic information of circular RNA
PanCircBase ID:
mmu_circAnln_1367
mm39_Chr. Position ID:
chr9|22262215|22275396|-
Gene name:
Anln
Transcript:
ENSMUST00000040912.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
12
Exon/intron indexes:
20,19,18,17,16,15,14,13,12,11,10,9
Exon/intron offsets:
0,956,1294,1863,2410,5132,7200,7693,9490,9902,11766,13070
Genomic length:
13181
Exon/intron sizes in nt:
113,22,39,57,49,127,183,136,148,146,236,111
Flanking exon/introns:
chr9:22250384-22262215|chr9:22275396-22279681
Splice length:
1367
Other database IDs:
circAtlas: mmu-Anln_0009
Human Ortholog IDs:
Mature spliced Sequence:
GTTCCACTGAAAGCGAAATGACAAAGTCCAGCCCTTTGAAAATCACGTTGTTTTTAGAAGAAGAAAAATCCTTAAAAGTAGCATCAGACCTGGAGGTTGAGCAGAACACTGAAGCAGTGCGTGAGGTTGAGATGAGTGTGGACGATGAGGACATCAATAGCTCCAGAGTCATTAACGACATCTTCAGTGACGTCCTAGAGGAAGGGGAGCTGGATGTGGAAAAGAGCCAAGAGGAGATGGACCAAGTGGGAGCAGAAAACAGTGAGGAGCAGGAAGATGCGCTCAATATCTCTTCAATGTCTTTACTTGCTCCGCTAGCTCAGACGGTCGGTGTGGTGAGCCTAGAGAATGTAATTTCTTCACCTCCGTCGGAATTGAGAGACTCTAACCTAAGCGCTGCAAGTCCTAAGCCCGGGAAATTCCAGAGAACCCGCGTCCCTCGCGCCGAATCTGCCGATAGCCTCGGTTCTGAGGACCGGGACCTTCTCTATAGCATTGATGCATATAGGTCTCAAAGATTCAAAGAAACAGAACGCCCTTCCATAAAGCAAGTGATTGTTCGAAAGGAAGATGTTACTTCAAAGTTGGGTGAAAAGAAAAACGTATTTTCTGGTCAAGTTAATATCAAACAAAAAATGCAGGAGCTCAATAATGACATAAATTTGCAGCAGACAGTGATCTATCAGGCCAGTCAGGCTCTCAACTGCTGTGTGGATGAAGAACACGGGAAAGGATCCTTGGAAGAAGCTGAAGCAGAAAGACTTCTTCTGATTGCAACTGAGAAAAGAGCACTTCTGATTGATGAGCTGAATAAGCTGAAGAGTGAAGGACCTCAGAGGAGAAACAAGACCAGTGTCATATCCCAGAGTGAATTTGCTCCATCTAAAGGGTCAGTCACTCTGTCAGAAATCTGCTTGCCTCTGAAGGCAGATTTTGTCTGCAGCACTGCGCAAAAAACAGATGCATCAAATTATTACTACTTAATTATGCTAAAAGCTGGGGCTGAGCAGATGGTCGCCACTCCATTAGCAAGTACTGCAAACTCTCTCAGTGGTGACGCTCTGACATTTCCTACTACATTTACTCTGCATGATGTTTCCAATGACTTTGAAATAAACATTGAAGTTTACAGCCTGGTACAAAAGAAAGATTCCTTGGGCCCCGATAAGAAGAAGAAAGCCTCCAAGTCCAAGGCTATTACTCCAAAGAGACTCCTCACATCTATAACTTCAAAAAGCAGCCTTCATTCTTCAGTTATGGCCAGTCCCGGAGGTCTCGGTGCTGTGCGTACCAGCAACTTTACCCTAGTTGGATCTCACACACTCTCCTTATCTTCTGTTGGAGACACTAAGTTTGCTTTGGACAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCGTACCAGCAACTTTACCC
59.2
CCTCCAGGTCTGATGCTACT
58.21
178
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GCGTACCAGCAACTTTACCC
59.2
CCTCCAGGTCTGATGCTACTT
58.89
178
PCR template sequence:
CGGAGGTCTCGGTGCTGTGCGTACCAGCAACTTTACCCTAGTTGGATCTCACACACTCTCCTTATCTTCTGTTGGAGACACTAAGTTTGCTTTGGACAAGGTTCCACTGAAAGCGAAATGACAAAGTCCAGCCCTTTGAAAATCACGTTGTTTTTAGAAGAAGAAAAATCCTTAAAAGTAGCATCAGACCTGGAGGTTGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-1904(2), mmu-miR-27a-5p(1), mmu-miR-325-3p(1), mmu-miR-129-5p(1), mmu-miR-574-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TGCTTTGGACAAGGTTCCACTGAAAG
siRNA sets
TTTCAGTGGAACCTTGTCC, CTTTCAGTGGAACCTTGTC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCANLN_1367_ORF_1
1.074
0.179390912
0.999336487
1371
ORF sequence:
ATGACAAAGTCCAGCCCTTTGAAAATCACGTTGTTTTTAGAAGAAGAAAAATCCTTAAAAGTAGCATCAGACCTGGAGGTTGAGCAGAACACTGAAGCAGTGCGTGAGGTTGAGATGAGTGTGGACGATGAGGACATCAATAGCTCCAGAGTCATTAACGACATCTTCAGTGACGTCCTAGAGGAAGGGGAGCTGGATGTGGAAAAGAGCCAAGAGGAGATGGACCAAGTGGGAGCAGAAAACAGTGAGGAGCAGGAAGATGCGCTCAATATCTCTTCAATGTCTTTACTTGCTCCGCTAGCTCAGACGGTCGGTGTGGTGAGCCTAGAGAATGTAATTTCTTCACCTCCGTCGGAATTGAGAGACTCTAACCTAAGCGCTGCAAGTCCTAAGCCCGGGAAATTCCAGAGAACCCGCGTCCCTCGCGCCGAATCTGCCGATAGCCTCGGTTCTGAGGACCGGGACCTTCTCTATAGCATTGATGCATATAGGTCTCAAAGATTCAAAGAAACAGAACGCCCTTCCATAAAGCAAGTGATTGTTCGAAAGGAAGATGTTACTTCAAAGTTGGGTGAAAAGAAAAACGTATTTTCTGGTCAAGTTAATATCAAACAAAAAATGCAGGAGCTCAATAATGACATAAATTTGCAGCAGACAGTGATCTATCAGGCCAGTCAGGCTCTCAACTGCTGTGTGGATGAAGAACACGGGAAAGGATCCTTGGAAGAAGCTGAAGCAGAAAGACTTCTTCTGATTGCAACTGAGAAAAGAGCACTTCTGATTGATGAGCTGAATAAGCTGAAGAGTGAAGGACCTCAGAGGAGAAACAAGACCAGTGTCATATCCCAGAGTGAATTTGCTCCATCTAAAGGGTCAGTCACTCTGTCAGAAATCTGCTTGCCTCTGAAGGCAGATTTTGTCTGCAGCACTGCGCAAAAAACAGATGCATCAAATTATTACTACTTAATTATGCTAAAAGCTGGGGCTGAGCAGATGGTCGCCACTCCATTAGCAAGTACTGCAAACTCTCTCAGTGGTGACGCTCTGACATTTCCTACTACATTTACTCTGCATGATGTTTCCAATGACTTTGAAATAAACATTGAAGTTTACAGCCTGGTACAAAAGAAAGATTCCTTGGGCCCCGATAAGAAGAAGAAAGCCTCCAAGTCCAAGGCTATTACTCCAAAGAGACTCCTCACATCTATAACTTCAAAAAGCAGCCTTCATTCTTCAGTTATGGCCAGTCCCGGAGGTCTCGGTGCTGTGCGTACCAGCAACTTTACCCTAGTTGGATCTCACACACTCTCCTTATCTTCTGTTGGAGACACTAAGTTTGCTTTGGACAAGGTTCCACTGAAAGCGAAATGA
Peptide sequence:
MTKSSPLKITLFLEEEKSLKVASDLEVEQNTEAVREVEMSVDDEDINSSRVINDIFSDVLEEGELDVEKSQEEMDQVGAENSEEQEDALNISSMSLLAPLAQTVGVVSLENVISSPPSELRDSNLSAASPKPGKFQRTRVPRAESADSLGSEDRDLLYSIDAYRSQRFKETERPSIKQVIVRKEDVTSKLGEKKNVFSGQVNIKQKMQELNNDINLQQTVIYQASQALNCCVDEEHGKGSLEEAEAERLLLIATEKRALLIDELNKLKSEGPQRRNKTSVISQSEFAPSKGSVTLSEICLPLKADFVCSTAQKTDASNYYYLIMLKAGAEQMVATPLASTANSLSGDALTFPTTFTLHDVSNDFEINIEVYSLVQKKDSLGPDKKKKASKSKAITPKRLLTSITSKSSLHSSVMASPGGLGAVRTSNFTLVGSHTLSLSSVGDTKFALDKVPLKAK
IRESfinder result
IRESfinder score
0.595569827
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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