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mmu_circAnln_1367

Basic information of circular RNA
PanCircBase ID:

mmu_circAnln_1367

mm39_Chr. Position ID:

chr9|22262215|22275396|-

Gene name:

Anln

Transcript:

ENSMUST00000040912.9

CircRNA type:

Exonic circular (circ)RNA

Exon/Intron count:

12

Exon/intron indexes:

20,19,18,17,16,15,14,13,12,11,10,9

Exon/intron offsets:

0,956,1294,1863,2410,5132,7200,7693,9490,9902,11766,13070

Genomic length:

13181

Exon/intron sizes in nt:

113,22,39,57,49,127,183,136,148,146,236,111

Flanking exon/introns:

chr9:22250384-22262215|chr9:22275396-22279681

Splice length:

1367

Other database IDs:

circAtlas: mmu-Anln_0009

Human Ortholog IDs:
Mature spliced Sequence:

GTTCCACTGAAAGCGAAATGACAAAGTCCAGCCCTTTGAAAATCACGTTGTTTTTAGAAGAAGAAAAATCCTTAAAAGTAGCATCAGACCTGGAGGTTGAGCAGAACACTGAAGCAGTGCGTGAGGTTGAGATGAGTGTGGACGATGAGGACATCAATAGCTCCAGAGTCATTAACGACATCTTCAGTGACGTCCTAGAGGAAGGGGAGCTGGATGTGGAAAAGAGCCAAGAGGAGATGGACCAAGTGGGAGCAGAAAACAGTGAGGAGCAGGAAGATGCGCTCAATATCTCTTCAATGTCTTTACTTGCTCCGCTAGCTCAGACGGTCGGTGTGGTGAGCCTAGAGAATGTAATTTCTTCACCTCCGTCGGAATTGAGAGACTCTAACCTAAGCGCTGCAAGTCCTAAGCCCGGGAAATTCCAGAGAACCCGCGTCCCTCGCGCCGAATCTGCCGATAGCCTCGGTTCTGAGGACCGGGACCTTCTCTATAGCATTGATGCATATAGGTCTCAAAGATTCAAAGAAACAGAACGCCCTTCCATAAAGCAAGTGATTGTTCGAAAGGAAGATGTTACTTCAAAGTTGGGTGAAAAGAAAAACGTATTTTCTGGTCAAGTTAATATCAAACAAAAAATGCAGGAGCTCAATAATGACATAAATTTGCAGCAGACAGTGATCTATCAGGCCAGTCAGGCTCTCAACTGCTGTGTGGATGAAGAACACGGGAAAGGATCCTTGGAAGAAGCTGAAGCAGAAAGACTTCTTCTGATTGCAACTGAGAAAAGAGCACTTCTGATTGATGAGCTGAATAAGCTGAAGAGTGAAGGACCTCAGAGGAGAAACAAGACCAGTGTCATATCCCAGAGTGAATTTGCTCCATCTAAAGGGTCAGTCACTCTGTCAGAAATCTGCTTGCCTCTGAAGGCAGATTTTGTCTGCAGCACTGCGCAAAAAACAGATGCATCAAATTATTACTACTTAATTATGCTAAAAGCTGGGGCTGAGCAGATGGTCGCCACTCCATTAGCAAGTACTGCAAACTCTCTCAGTGGTGACGCTCTGACATTTCCTACTACATTTACTCTGCATGATGTTTCCAATGACTTTGAAATAAACATTGAAGTTTACAGCCTGGTACAAAAGAAAGATTCCTTGGGCCCCGATAAGAAGAAGAAAGCCTCCAAGTCCAAGGCTATTACTCCAAAGAGACTCCTCACATCTATAACTTCAAAAAGCAGCCTTCATTCTTCAGTTATGGCCAGTCCCGGAGGTCTCGGTGCTGTGCGTACCAGCAACTTTACCCTAGTTGGATCTCACACACTCTCCTTATCTTCTGTTGGAGACACTAAGTTTGCTTTGGACAAG

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

GCGTACCAGCAACTTTACCC

59.2

CCTCCAGGTCTGATGCTACT

58.21

178

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

GCGTACCAGCAACTTTACCC

59.2

CCTCCAGGTCTGATGCTACTT

58.89

178

PCR template sequence:

CGGAGGTCTCGGTGCTGTGCGTACCAGCAACTTTACCCTAGTTGGATCTCACACACTCTCCTTATCTTCTGTTGGAGACACTAAGTTTGCTTTGGACAAGGTTCCACTGAAAGCGAAATGACAAAGTCCAGCCCTTTGAAAATCACGTTGTTTTTAGAAGAAGAAAAATCCTTAAAAGTAGCATCAGACCTGGAGGTTGA

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

5

Interacting miRNAs (#binding sites)

mmu-miR-1904(2), mmu-miR-27a-5p(1), mmu-miR-325-3p(1), mmu-miR-129-5p(1), mmu-miR-574-5p(1)

siRNAs for circular RNA silencing
Junction sequence

TGCTTTGGACAAGGTTCCACTGAAAG

siRNA sets

TTTCAGTGGAACCTTGTCC, CTTTCAGTGGAACCTTGTC

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

CIRCANLN_1367_ORF_1

1.074

0.179390912

0.999336487

1371

ORF sequence:

ATGACAAAGTCCAGCCCTTTGAAAATCACGTTGTTTTTAGAAGAAGAAAAATCCTTAAAAGTAGCATCAGACCTGGAGGTTGAGCAGAACACTGAAGCAGTGCGTGAGGTTGAGATGAGTGTGGACGATGAGGACATCAATAGCTCCAGAGTCATTAACGACATCTTCAGTGACGTCCTAGAGGAAGGGGAGCTGGATGTGGAAAAGAGCCAAGAGGAGATGGACCAAGTGGGAGCAGAAAACAGTGAGGAGCAGGAAGATGCGCTCAATATCTCTTCAATGTCTTTACTTGCTCCGCTAGCTCAGACGGTCGGTGTGGTGAGCCTAGAGAATGTAATTTCTTCACCTCCGTCGGAATTGAGAGACTCTAACCTAAGCGCTGCAAGTCCTAAGCCCGGGAAATTCCAGAGAACCCGCGTCCCTCGCGCCGAATCTGCCGATAGCCTCGGTTCTGAGGACCGGGACCTTCTCTATAGCATTGATGCATATAGGTCTCAAAGATTCAAAGAAACAGAACGCCCTTCCATAAAGCAAGTGATTGTTCGAAAGGAAGATGTTACTTCAAAGTTGGGTGAAAAGAAAAACGTATTTTCTGGTCAAGTTAATATCAAACAAAAAATGCAGGAGCTCAATAATGACATAAATTTGCAGCAGACAGTGATCTATCAGGCCAGTCAGGCTCTCAACTGCTGTGTGGATGAAGAACACGGGAAAGGATCCTTGGAAGAAGCTGAAGCAGAAAGACTTCTTCTGATTGCAACTGAGAAAAGAGCACTTCTGATTGATGAGCTGAATAAGCTGAAGAGTGAAGGACCTCAGAGGAGAAACAAGACCAGTGTCATATCCCAGAGTGAATTTGCTCCATCTAAAGGGTCAGTCACTCTGTCAGAAATCTGCTTGCCTCTGAAGGCAGATTTTGTCTGCAGCACTGCGCAAAAAACAGATGCATCAAATTATTACTACTTAATTATGCTAAAAGCTGGGGCTGAGCAGATGGTCGCCACTCCATTAGCAAGTACTGCAAACTCTCTCAGTGGTGACGCTCTGACATTTCCTACTACATTTACTCTGCATGATGTTTCCAATGACTTTGAAATAAACATTGAAGTTTACAGCCTGGTACAAAAGAAAGATTCCTTGGGCCCCGATAAGAAGAAGAAAGCCTCCAAGTCCAAGGCTATTACTCCAAAGAGACTCCTCACATCTATAACTTCAAAAAGCAGCCTTCATTCTTCAGTTATGGCCAGTCCCGGAGGTCTCGGTGCTGTGCGTACCAGCAACTTTACCCTAGTTGGATCTCACACACTCTCCTTATCTTCTGTTGGAGACACTAAGTTTGCTTTGGACAAGGTTCCACTGAAAGCGAAATGA

Peptide sequence:

MTKSSPLKITLFLEEEKSLKVASDLEVEQNTEAVREVEMSVDDEDINSSRVINDIFSDVLEEGELDVEKSQEEMDQVGAENSEEQEDALNISSMSLLAPLAQTVGVVSLENVISSPPSELRDSNLSAASPKPGKFQRTRVPRAESADSLGSEDRDLLYSIDAYRSQRFKETERPSIKQVIVRKEDVTSKLGEKKNVFSGQVNIKQKMQELNNDINLQQTVIYQASQALNCCVDEEHGKGSLEEAEAERLLLIATEKRALLIDELNKLKSEGPQRRNKTSVISQSEFAPSKGSVTLSEICLPLKADFVCSTAQKTDASNYYYLIMLKAGAEQMVATPLASTANSLSGDALTFPTTFTLHDVSNDFEINIEVYSLVQKKDSLGPDKKKKASKSKAITPKRLLTSITSKSSLHSSVMASPGGLGAVRTSNFTLVGSHTLSLSSVGDTKFALDKVPLKAK

IRESfinder result

IRESfinder score

0.595569827

IRES/non IRES

IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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