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mmu_circAnks3_416
Basic information of circular RNA
PanCircBase ID:
mmu_circAnks3_416
mm39_Chr. Position ID:
chr16|4765128|4766227|-
Gene name:
Anks3
Transcript:
ENSMUST00000023157.6
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
10,9,8
Exon/intron offsets:
0,384,961
Genomic length:
1099
Exon/intron sizes in nt:
16,81,10,138
Flanking exon/introns:
chr16:4764043-4765128|chr16:4766227-4768476
Splice length:
416
Other database IDs:
circAtlas: mmu-Anks3_0014
circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_167514
Human Ortholog IDs:
Mature spliced Sequence:
AGCAAGTCCCTCCACGGGGCTACGTGACCTTCACCAGCAGTGATGAGAATACCATGGAAAGCGAGGGCCTCTGCTACAGAGACGTCACTTCCCCCATCAATGAACAGGATGTGGAGAGCAGTAGCAGCAGCAGCCGGGAGGAACCCACTTTCTGTGCCAGTCTTGGGCCTGTGTGGAGGAGCAGCAGCAGTGATGGCCTAGCAAGAGCCCAGGGTCTCAGCAGTGAAGCCTCCATAGAAAGCAATGAGGATTCTGATCATGCCCGGAAAAGCTCGGTTCGAAAACAAACCAGGAGTTATTTGAAGAATAAGAGTCGTCACGGCAACAGTGATGGTCATTGGCCTTCCAGCACAGGGCCTGCTTCCATTCCAGGGTCTGAACCCCAAACTGAGAAGTCTCCTTACTCAGGACCCCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACGGCAACAGTGATGGTCAT
59.96
GCCCTCGCTTTCCATGGTAT
60.18
166
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGCTTCCATTCCAGGGTCTG
59.67
GATGGGGGAAGTGACGTCTC
59.82
156
PCR template sequence:
TCACGGCAACAGTGATGGTCATTGGCCTTCCAGCACAGGGCCTGCTTCCATTCCAGGGTCTGAACCCCAAACTGAGAAGTCTCCTTACTCAGGACCCCAGAGCAAGTCCCTCCACGGGGCTACGTGACCTTCACCAGCAGTGATGAGAATACCATGGAAAGCGAGGGCCTCTGCTACAGAGACGTCACTTCCCCCATCAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-431-5p(1), mmu-miR-335-3p(1), mmu-miR-697(1)
siRNAs for circular RNA silencing
Junction sequence
CTCAGGACCCCAGAGCAAGTCCCTCC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCANKS3_416_ORF_1
1.1792
0.19582294
0.633054779
390
ORF sequence:
ATGGAAAGCGAGGGCCTCTGCTACAGAGACGTCACTTCCCCCATCAATGAACAGGATGTGGAGAGCAGTAGCAGCAGCAGCCGGGAGGAACCCACTTTCTGTGCCAGTCTTGGGCCTGTGTGGAGGAGCAGCAGCAGTGATGGCCTAGCAAGAGCCCAGGGTCTCAGCAGTGAAGCCTCCATAGAAAGCAATGAGGATTCTGATCATGCCCGGAAAAGCTCGGTTCGAAAACAAACCAGGAGTTATTTGAAGAATAAGAGTCGTCACGGCAACAGTGATGGTCATTGGCCTTCCAGCACAGGGCCTGCTTCCATTCCAGGGTCTGAACCCCAAACTGAGAAGTCTCCTTACTCAGGACCCCAGAGCAAGTCCCTCCACGGGGCTACGTGA
Peptide sequence:
MESEGLCYRDVTSPINEQDVESSSSSSREEPTFCASLGPVWRSSSSDGLARAQGLSSEASIESNEDSDHARKSSVRKQTRSYLKNKSRHGNSDGHWPSSTGPASIPGSEPQTEKSPYSGPQSKSLHGAT
IRESfinder result
IRESfinder score
0.497415973
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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