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mmu_circAnks1_87
Basic information of circular RNA
PanCircBase ID:
mmu_circAnks1_87
mm39_Chr. Position ID:
chr17|28273643|28273730|+
Gene name:
Anks1
Transcript:
ENSMUST00000114842.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
1
Exon/intron indexes:
21
Exon/intron offsets:
0
Genomic length:
87
Exon/intron sizes in nt:
87
Flanking exon/introns:
chr17:28273337-28273643|chr17:28273730-28276300
Splice length:
87
Other database IDs:
circBase: hsa_circ_0076087, CIRCpedia: HSA_CIRCpedia_54824
Human Ortholog IDs:
Mature spliced Sequence:
AAGTCAACGGAGCACATGAAGAAGATCCCCACCATCATCCTGTCCATCACGTACAAAGGTGTCAAGTTCATCGATGCTTCCAACAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGGTGTCAAGTTCATCGATGCT
60.03
ACAGGATGATGGTGGGGATC
58.86
74
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGGTGTCAAGTTCATCGATGCT
60.03
ACAGGATGATGGTGGGGATCT
60.35
74
PCR template sequence:
CATCACGTACAAAGGTGTCAAGTTCATCGATGCTTCCAACAAGAAGTCAACGGAGCACATGAAGAAGATCCCCACCATCATCCTGT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
NA
Interacting miRNAs (#binding sites)
NA
siRNAs for circular RNA silencing
Junction sequence
TGCTTCCAACAAGAAGTCAACGGAGC
siRNA sets
TCCGTTGACTTCTTGTTGG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCANKS1_87_ORF_1
1.2763
0.741513513
0.786740255
159
ORF sequence:
ATGAAGAAGATCCCCACCATCATCCTGTCCATCACGTACAAAGGTGTCAAGTTCATCGATGCTTCCAACAAGAAGTCAACGGAGCACATGAAGAAGATCCCCACCATCATCCTGTCCATCACGTACAAAGGTGTCAAGTTCATCGATGCTTCCAACAAG
Peptide sequence:
MKKIPTIILSITYKGVKFIDASNKKSTEHMKKIPTIILSITYKGVKFIDASNK
IRESfinder result
IRESfinder score
0.436839773
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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