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mmu_circAnks1_2259
Basic information of circular RNA
PanCircBase ID:
mmu_circAnks1_2259
mm39_Chr. Position ID:
chr17|28205225|28273730|+
Gene name:
Anks1
Transcript:
ENSMUST00000114842.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
16
Exon/intron indexes:
6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21
Exon/intron offsets:
0,299,493,2468,5056,9682,21597,51828,56318,64314,65245,65473,66387,67638,68040,68418
Genomic length:
68505
Exon/intron sizes in nt:
76,102,102,197,93,121,584,67,107,99,153,108,165,126,72,87
Flanking exon/introns:
chr17:28204336-28205225|chr17:28273730-28276300
Splice length:
2259
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
ACGGAGATGGGCAGTGCTTTGCATGAGGCTGCTTTGTTTGGCAAGACCGATGTGGTGCAAATCCTGCTGGCTGCAGGAATCGATGTTAACATAAAGGATAACCGTGGCCTGACTGCCCTTGACACTGTCCGGGATCTGCCCTCTCAGAAGAGCCAGCAGATAGCCGCCCTGATTGAAGATCACATGACCGGAAAAAGAAGCGTGAAAGAAGTGGACAGGACCTCCACAGCCCAGCTACCTCTCCTCTCCAACACAGACGCCATAGCACCAATGTCTCAGGGGAGTATGGAGAAGACAGTGACTGAGCTGATCCTTCATTTTGACACACATGCTGACGAGGAGGGCCCCTACGAGGCACTGTACAACGCTGTGTCCTGCCATTCCTTGGACAGCACGGCCAGTGGACGATCATCAGACAGAGACTCCATGAACAAGGAGGCCGAGGCCACCGGAACAAGGGCTGCTGGAGTGAGGCCTAGGGAACGTCCGCCGCCTCCAGCAAAACCGCCACCCGATGAAGAGGAGGAAGAACGCGTAGATAAGAAGTATTTTCCCTTGGCAGCCTCTGAGGGCCTGGCTGTGAGACCCAGGATTCAGAGTAGTGCTCCCCAGGAAGAGGAGGAGCACCCATATGAGCTGCTGCTCACGGCAGAAACCAAGAAGCTGGGGACCACAGACGGGAGGACTGAAGACCACAGGCAGAGCGGCAGCGGCCGGAGCCAGGACTCTGTGGAGGGGCAGGACGGGCAGGTCCCAGAGCAGTTCTCAGGTCTCCTCCACGGCTCCTCCCCAGTGTGTGAGGTGGGGCAGGACCCTTTCCAGCTGCTCACGGCCCCCAGCCAGAGCCACCCGGAGTCCTCCCAGCAGGACGCCTGCCACGAGGCCAGCATGCAGCTAGAGGAGCCAGGCGTGCAAGGCACTGAACCCCCGCAGCCCGGCGTCCCTGACCAAAGCAAGAGAGTGGGCTTGCCGGCAGGCCTGACAGCCCTGGCCAGCAGAACATACCTCGATGCATTGACTCACACAGTACCTCTGCGCCCTGCTGGTGCCGAGGAAGAAGACCAGAGCGGGCCCAGAAGCAGAGCCCCTCCCACCAGCAAGCCCAAAGCTGAGCTCAAACTCAGCCGCAGTTTGTCTAAGTCTGACTCTGACCTCCTGACCTGCTCCCCCACGGAGGACGCCACAATGGGGAGTCGCAGCGAGTCCTTGTCCAACTGCAGCATCGGGAAGAAGAGGTTGGAGAAATCACCTTCCTTTGCCTCAGAGTGGGATGAGATTGAGAAAATCATGAGCTCCATTGGGGAAGGGATTGACTTCTCTCAGGAGCAGCAGAAGATCTCAGGTTCTCGGACGCTGGAGCAGAGCGTCGGGGAGTGGCTGGAGTCGATTGGCCTGCAGCAGTACGAGAGCAAGCTGCTGCTGAACGGCTTTGATGATGTCCGCTTCCTGGGGTCTAATGTGATGGAAGAGCAGGACCTGAGGGAGATTGGCATCAGTGACCCACAGCACCGGCGGAAGCTGCTGCAGGCTGCACGCTCCTTGCCCAAGGTGAAGGCCCTTGGCTATGACGGGGTCAGCCCCACCTCAGTGCCCTCATGGCTGGACTCGCTGGGGCTGCAGGACTATGTCCACTCTTTCCTGTCCAGTGGCTACAGCTCCATCGACACTGTGAAGAACCTCTGGGAGCTAGAGCTTGTCAATGTCCTGAAAGTTCACCTGCTGGGCCACCGCAAGCGCATTATCGCCTCTCTAGCAGACAGGCCCTATGAGGAGCCACCCCAGAAGCCTCCAAGGTTTTCCCAGCTAAGATGCCAGGATTTAATCTCCCAGACATCGTCCCCGCTGAGCCAGAATGATTCCTGCACAGGGCGGTCGGCAGACCTGCTGCTGCCCTCAGCAGACACAAGCAGGAGGCGGCACGACAGCCTGCCCGACCCTGGGACCGCCTCCCGAGCAGACCGCTTCCGAGTCCAGGAGGAGCCCAGTGAGACCAAGCTGACCCTCCGTCCACCCAGCCTAGCCGCGCCCTATGCTCCTGTTCAGAGCTGGCAGCACCAGCCAGAAAAGCTCATCTTTGAGTCCTGCGGTTATGAGGCCAATTATCTGGGTTCCATGCTGATCAAAGATCTGCGAGGGACAGAGTCCACCCAAGATGCCTGTGCCAAAATGCGGAAGTCAACGGAGCACATGAAGAAGATCCCCACCATCATCCTGTCCATCACGTACAAAGGTGTCAAGTTCATCGATGCTTCCAACAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CGGAAGTCAACGGAGCACAT
60.67
AACATCGATTCCTGCAGCCA
60.04
177
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGAAGTCAACGGAGCACATG
59.2
AACATCGATTCCTGCAGCCA
60.04
176
PCR template sequence:
TGCCAAAATGCGGAAGTCAACGGAGCACATGAAGAAGATCCCCACCATCATCCTGTCCATCACGTACAAAGGTGTCAAGTTCATCGATGCTTCCAACAAGACGGAGATGGGCAGTGCTTTGCATGAGGCTGCTTTGTTTGGCAAGACCGATGTGGTGCAAATCCTGCTGGCTGCAGGAATCGATGTTAACATAAAGGATA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
37
Interacting miRNAs (#binding sites)
mmu-miR-322-5p(2), mmu-miR-138-5p(2), mmu-miR-149-5p(2), mmu-miR-23b-3p(1), mmu-let-7a-5p(1), mmu-miR-16-5p(1), mmu-miR-15a-5p(1), mmu-miR-27b-3p(1), mmu-miR-27a-3p(1), mmu-miR-199a-5p(1), mmu-let-7e-5p(1), mmu-let-7b-5p(1), mmu-let-7c-5p(1), mmu-miR-15b-5p(1), mmu-miR-146a-5p(1), mmu-miR-298-5p(1), mmu-miR-709(1), mmu-miR-326-3p(1), mmu-miR-125b-5p(1), mmu-miR-23a-3p(1), mmu-miR-185-5p(1), mmu-miR-214-3p(1), mmu-let-7d-5p(1), mmu-miR-125a-5p(1), mmu-miR-96-5p(1), mmu-miR-370-3p(1), mmu-miR-98-5p(1), mmu-miR-466a-5p(1), mmu-miR-1187(1), mmu-miR-466p-5p(1), mmu-miR-207(1), mmu-miR-673-5p(1), mmu-miR-669d-5p(1), mmu-miR-452-3p(1), mmu-miR-3470b(1), mmu-miR-540-3p(1), mmu-miR-1904(1)
siRNAs for circular RNA silencing
Junction sequence
TGCTTCCAACAAGACGGAGATGGGCA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCANKS1_2259_ORF_1
1.112
0.433711263
1
4512
ORF sequence:
ATGGGCAGTGCTTTGCATGAGGCTGCTTTGTTTGGCAAGACCGATGTGGTGCAAATCCTGCTGGCTGCAGGAATCGATGTTAACATAAAGGATAACCGTGGCCTGACTGCCCTTGACACTGTCCGGGATCTGCCCTCTCAGAAGAGCCAGCAGATAGCCGCCCTGATTGAAGATCACATGACCGGAAAAAGAAGCGTGAAAGAAGTGGACAGGACCTCCACAGCCCAGCTACCTCTCCTCTCCAACACAGACGCCATAGCACCAATGTCTCAGGGGAGTATGGAGAAGACAGTGACTGAGCTGATCCTTCATTTTGACACACATGCTGACGAGGAGGGCCCCTACGAGGCACTGTACAACGCTGTGTCCTGCCATTCCTTGGACAGCACGGCCAGTGGACGATCATCAGACAGAGACTCCATGAACAAGGAGGCCGAGGCCACCGGAACAAGGGCTGCTGGAGTGAGGCCTAGGGAACGTCCGCCGCCTCCAGCAAAACCGCCACCCGATGAAGAGGAGGAAGAACGCGTAGATAAGAAGTATTTTCCCTTGGCAGCCTCTGAGGGCCTGGCTGTGAGACCCAGGATTCAGAGTAGTGCTCCCCAGGAAGAGGAGGAGCACCCATATGAGCTGCTGCTCACGGCAGAAACCAAGAAGCTGGGGACCACAGACGGGAGGACTGAAGACCACAGGCAGAGCGGCAGCGGCCGGAGCCAGGACTCTGTGGAGGGGCAGGACGGGCAGGTCCCAGAGCAGTTCTCAGGTCTCCTCCACGGCTCCTCCCCAGTGTGTGAGGTGGGGCAGGACCCTTTCCAGCTGCTCACGGCCCCCAGCCAGAGCCACCCGGAGTCCTCCCAGCAGGACGCCTGCCACGAGGCCAGCATGCAGCTAGAGGAGCCAGGCGTGCAAGGCACTGAACCCCCGCAGCCCGGCGTCCCTGACCAAAGCAAGAGAGTGGGCTTGCCGGCAGGCCTGACAGCCCTGGCCAGCAGAACATACCTCGATGCATTGACTCACACAGTACCTCTGCGCCCTGCTGGTGCCGAGGAAGAAGACCAGAGCGGGCCCAGAAGCAGAGCCCCTCCCACCAGCAAGCCCAAAGCTGAGCTCAAACTCAGCCGCAGTTTGTCTAAGTCTGACTCTGACCTCCTGACCTGCTCCCCCACGGAGGACGCCACAATGGGGAGTCGCAGCGAGTCCTTGTCCAACTGCAGCATCGGGAAGAAGAGGTTGGAGAAATCACCTTCCTTTGCCTCAGAGTGGGATGAGATTGAGAAAATCATGAGCTCCATTGGGGAAGGGATTGACTTCTCTCAGGAGCAGCAGAAGATCTCAGGTTCTCGGACGCTGGAGCAGAGCGTCGGGGAGTGGCTGGAGTCGATTGGCCTGCAGCAGTACGAGAGCAAGCTGCTGCTGAACGGCTTTGATGATGTCCGCTTCCTGGGGTCTAATGTGATGGAAGAGCAGGACCTGAGGGAGATTGGCATCAGTGACCCACAGCACCGGCGGAAGCTGCTGCAGGCTGCACGCTCCTTGCCCAAGGTGAAGGCCCTTGGCTATGACGGGGTCAGCCCCACCTCAGTGCCCTCATGGCTGGACTCGCTGGGGCTGCAGGACTATGTCCACTCTTTCCTGTCCAGTGGCTACAGCTCCATCGACACTGTGAAGAACCTCTGGGAGCTAGAGCTTGTCAATGTCCTGAAAGTTCACCTGCTGGGCCACCGCAAGCGCATTATCGCCTCTCTAGCAGACAGGCCCTATGAGGAGCCACCCCAGAAGCCTCCAAGGTTTTCCCAGCTAAGATGCCAGGATTTAATCTCCCAGACATCGTCCCCGCTGAGCCAGAATGATTCCTGCACAGGGCGGTCGGCAGACCTGCTGCTGCCCTCAGCAGACACAAGCAGGAGGCGGCACGACAGCCTGCCCGACCCTGGGACCGCCTCCCGAGCAGACCGCTTCCGAGTCCAGGAGGAGCCCAGTGAGACCAAGCTGACCCTCCGTCCACCCAGCCTAGCCGCGCCCTATGCTCCTGTTCAGAGCTGGCAGCACCAGCCAGAAAAGCTCATCTTTGAGTCCTGCGGTTATGAGGCCAATTATCTGGGTTCCATGCTGATCAAAGATCTGCGAGGGACAGAGTCCACCCAAGATGCCTGTGCCAAAATGCGGAAGTCAACGGAGCACATGAAGAAGATCCCCACCATCATCCTGTCCATCACGTACAAAGGTGTCAAGTTCATCGATGCTTCCAACAAGACGGAGATGGGCAGTGCTTTGCATGAGGCTGCTTTGTTTGGCAAGACCGATGTGGTGCAAATCCTGCTGGCTGCAGGAATCGATGTTAACATAAAGGATAACCGTGGCCTGACTGCCCTTGACACTGTCCGGGATCTGCCCTCTCAGAAGAGCCAGCAGATAGCCGCCCTGATTGAAGATCACATGACCGGAAAAAGAAGCGTGAAAGAAGTGGACAGGACCTCCACAGCCCAGCTACCTCTCCTCTCCAACACAGACGCCATAGCACCAATGTCTCAGGGGAGTATGGAGAAGACAGTGACTGAGCTGATCCTTCATTTTGACACACATGCTGACGAGGAGGGCCCCTACGAGGCACTGTACAACGCTGTGTCCTGCCATTCCTTGGACAGCACGGCCAGTGGACGATCATCAGACAGAGACTCCATGAACAAGGAGGCCGAGGCCACCGGAACAAGGGCTGCTGGAGTGAGGCCTAGGGAACGTCCGCCGCCTCCAGCAAAACCGCCACCCGATGAAGAGGAGGAAGAACGCGTAGATAAGAAGTATTTTCCCTTGGCAGCCTCTGAGGGCCTGGCTGTGAGACCCAGGATTCAGAGTAGTGCTCCCCAGGAAGAGGAGGAGCACCCATATGAGCTGCTGCTCACGGCAGAAACCAAGAAGCTGGGGACCACAGACGGGAGGACTGAAGACCACAGGCAGAGCGGCAGCGGCCGGAGCCAGGACTCTGTGGAGGGGCAGGACGGGCAGGTCCCAGAGCAGTTCTCAGGTCTCCTCCACGGCTCCTCCCCAGTGTGTGAGGTGGGGCAGGACCCTTTCCAGCTGCTCACGGCCCCCAGCCAGAGCCACCCGGAGTCCTCCCAGCAGGACGCCTGCCACGAGGCCAGCATGCAGCTAGAGGAGCCAGGCGTGCAAGGCACTGAACCCCCGCAGCCCGGCGTCCCTGACCAAAGCAAGAGAGTGGGCTTGCCGGCAGGCCTGACAGCCCTGGCCAGCAGAACATACCTCGATGCATTGACTCACACAGTACCTCTGCGCCCTGCTGGTGCCGAGGAAGAAGACCAGAGCGGGCCCAGAAGCAGAGCCCCTCCCACCAGCAAGCCCAAAGCTGAGCTCAAACTCAGCCGCAGTTTGTCTAAGTCTGACTCTGACCTCCTGACCTGCTCCCCCACGGAGGACGCCACAATGGGGAGTCGCAGCGAGTCCTTGTCCAACTGCAGCATCGGGAAGAAGAGGTTGGAGAAATCACCTTCCTTTGCCTCAGAGTGGGATGAGATTGAGAAAATCATGAGCTCCATTGGGGAAGGGATTGACTTCTCTCAGGAGCAGCAGAAGATCTCAGGTTCTCGGACGCTGGAGCAGAGCGTCGGGGAGTGGCTGGAGTCGATTGGCCTGCAGCAGTACGAGAGCAAGCTGCTGCTGAACGGCTTTGATGATGTCCGCTTCCTGGGGTCTAATGTGATGGAAGAGCAGGACCTGAGGGAGATTGGCATCAGTGACCCACAGCACCGGCGGAAGCTGCTGCAGGCTGCACGCTCCTTGCCCAAGGTGAAGGCCCTTGGCTATGACGGGGTCAGCCCCACCTCAGTGCCCTCATGGCTGGACTCGCTGGGGCTGCAGGACTATGTCCACTCTTTCCTGTCCAGTGGCTACAGCTCCATCGACACTGTGAAGAACCTCTGGGAGCTAGAGCTTGTCAATGTCCTGAAAGTTCACCTGCTGGGCCACCGCAAGCGCATTATCGCCTCTCTAGCAGACAGGCCCTATGAGGAGCCACCCCAGAAGCCTCCAAGGTTTTCCCAGCTAAGATGCCAGGATTTAATCTCCCAGACATCGTCCCCGCTGAGCCAGAATGATTCCTGCACAGGGCGGTCGGCAGACCTGCTGCTGCCCTCAGCAGACACAAGCAGGAGGCGGCACGACAGCCTGCCCGACCCTGGGACCGCCTCCCGAGCAGACCGCTTCCGAGTCCAGGAGGAGCCCAGTGAGACCAAGCTGACCCTCCGTCCACCCAGCCTAGCCGCGCCCTATGCTCCTGTTCAGAGCTGGCAGCACCAGCCAGAAAAGCTCATCTTTGAGTCCTGCGGTTATGAGGCCAATTATCTGGGTTCCATGCTGATCAAAGATCTGCGAGGGACAGAGTCCACCCAAGATGCCTGTGCCAAAATGCGGAAGTCAACGGAGCACATGAAGAAGATCCCCACCATCATCCTGTCCATCACGTACAAAGGTGTCAAGTTCATCGATGCTTCCAACAAG
Peptide sequence:
MGSALHEAALFGKTDVVQILLAAGIDVNIKDNRGLTALDTVRDLPSQKSQQIAALIEDHMTGKRSVKEVDRTSTAQLPLLSNTDAIAPMSQGSMEKTVTELILHFDTHADEEGPYEALYNAVSCHSLDSTASGRSSDRDSMNKEAEATGTRAAGVRPRERPPPPAKPPPDEEEEERVDKKYFPLAASEGLAVRPRIQSSAPQEEEEHPYELLLTAETKKLGTTDGRTEDHRQSGSGRSQDSVEGQDGQVPEQFSGLLHGSSPVCEVGQDPFQLLTAPSQSHPESSQQDACHEASMQLEEPGVQGTEPPQPGVPDQSKRVGLPAGLTALASRTYLDALTHTVPLRPAGAEEEDQSGPRSRAPPTSKPKAELKLSRSLSKSDSDLLTCSPTEDATMGSRSESLSNCSIGKKRLEKSPSFASEWDEIEKIMSSIGEGIDFSQEQQKISGSRTLEQSVGEWLESIGLQQYESKLLLNGFDDVRFLGSNVMEEQDLREIGISDPQHRRKLLQAARSLPKVKALGYDGVSPTSVPSWLDSLGLQDYVHSFLSSGYSSIDTVKNLWELELVNVLKVHLLGHRKRIIASLADRPYEEPPQKPPRFSQLRCQDLISQTSSPLSQNDSCTGRSADLLLPSADTSRRRHDSLPDPGTASRADRFRVQEEPSETKLTLRPPSLAAPYAPVQSWQHQPEKLIFESCGYEANYLGSMLIKDLRGTESTQDACAKMRKSTEHMKKIPTIILSITYKGVKFIDASNKTEMGSALHEAALFGKTDVVQILLAAGIDVNIKDNRGLTALDTVRDLPSQKSQQIAALIEDHMTGKRSVKEVDRTSTAQLPLLSNTDAIAPMSQGSMEKTVTELILHFDTHADEEGPYEALYNAVSCHSLDSTASGRSSDRDSMNKEAEATGTRAAGVRPRERPPPPAKPPPDEEEEERVDKKYFPLAASEGLAVRPRIQSSAPQEEEEHPYELLLTAETKKLGTTDGRTEDHRQSGSGRSQDSVEGQDGQVPEQFSGLLHGSSPVCEVGQDPFQLLTAPSQSHPESSQQDACHEASMQLEEPGVQGTEPPQPGVPDQSKRVGLPAGLTALASRTYLDALTHTVPLRPAGAEEEDQSGPRSRAPPTSKPKAELKLSRSLSKSDSDLLTCSPTEDATMGSRSESLSNCSIGKKRLEKSPSFASEWDEIEKIMSSIGEGIDFSQEQQKISGSRTLEQSVGEWLESIGLQQYESKLLLNGFDDVRFLGSNVMEEQDLREIGISDPQHRRKLLQAARSLPKVKALGYDGVSPTSVPSWLDSLGLQDYVHSFLSSGYSSIDTVKNLWELELVNVLKVHLLGHRKRIIASLADRPYEEPPQKPPRFSQLRCQDLISQTSSPLSQNDSCTGRSADLLLPSADTSRRRHDSLPDPGTASRADRFRVQEEPSETKLTLRPPSLAAPYAPVQSWQHQPEKLIFESCGYEANYLGSMLIKDLRGTESTQDACAKMRKSTEHMKKIPTIILSITYKGVKFIDASNK
IRESfinder result
IRESfinder score
0.321211961
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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