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mmu_circAnkrd44_403
Basic information of circular RNA
PanCircBase ID:
mmu_circAnkrd44_403
mm39_Chr. Position ID:
chr1|54762172|54772155|-
Gene name:
Ankrd44
Transcript:
ENSMUST00000179030.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
16,15,14,13
Exon/intron offsets:
0,4476,6720,9914
Genomic length:
9983
Exon/intron sizes in nt:
112,81,141,69
Flanking exon/introns:
chr1:54720350-54762172|chr1:54772155-54774154
Splice length:
403
Other database IDs:
circAtlas: mmu-Ankrd44_0022
circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_158060
Human Ortholog IDs:
Mature spliced Sequence:
CAATGTAGAATGCATAAAGCTCTTGCAGAGCAGCGGAGCAGATTTTCACAAAAAGGACAAATGTGGGAGAACCCCATTGCACTATGCAGCTGCAAATTGCCATTTCCACTGTATTAAAGCATTAGTGACCACCGGAGCCAACGTTAATGAAACAGACGACTGGGGACGGACAGCTCTGCACTACGCTGCTGCATCAGACATGGATAGAAATAAGATGATCTTAGGAAATGCCCATGACAATTCTGAAGAACTTGAAAGAGCCAGAGAAGTGAAGGAAAAAGATGCTGCACTATGTCTAGAATTTCTGCTTCAAAATGATGCAAACCCATCCATCCGGGACAAGGAAGGCTACAATAGCATCCACTATGCTGCTGCCTATGGCCACAGACAGTGTCTAGAACTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CGGGACAAGGAAGGCTACAA
59.68
GCATAGTGCAATGGGGTTCTC
59.32
156
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ATCCGGGACAAGGAAGGCTA
60.33
GCATAGTGCAATGGGGTTCTC
59.32
159
PCR template sequence:
TCTGCTTCAAAATGATGCAAACCCATCCATCCGGGACAAGGAAGGCTACAATAGCATCCACTATGCTGCTGCCTATGGCCACAGACAGTGTCTAGAACTGCAATGTAGAATGCATAAAGCTCTTGCAGAGCAGCGGAGCAGATTTTCACAAAAAGGACAAATGTGGGAGAACCCCATTGCACTATGCAGCTGCAAATTGC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
11
Interacting miRNAs (#binding sites)
mmu-miR-149-5p(2), mmu-miR-155-5p(1), mmu-miR-15a-5p(1), mmu-miR-103-3p(1), mmu-miR-322-5p(1), mmu-miR-130a-3p(1), mmu-miR-503-5p(1), mmu-miR-130b-3p(1), mmu-miR-301b-3p(1), mmu-miR-107-3p(1), mmu-miR-301a-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GTGTCTAGAACTGCAATGTAGAATGC
siRNA sets
ACATTGCAGTTCTAGACAC, ATTCTACATTGCAGTTCTA, TTCTACATTGCAGTTCTAG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCANKRD44_403_ORF_1
0.7418
0.069355378
0.217127319
321
ORF sequence:
ATGGATAGAAATAAGATGATCTTAGGAAATGCCCATGACAATTCTGAAGAACTTGAAAGAGCCAGAGAAGTGAAGGAAAAAGATGCTGCACTATGTCTAGAATTTCTGCTTCAAAATGATGCAAACCCATCCATCCGGGACAAGGAAGGCTACAATAGCATCCACTATGCTGCTGCCTATGGCCACAGACAGTGTCTAGAACTGCAATGTAGAATGCATAAAGCTCTTGCAGAGCAGCGGAGCAGATTTTCACAAAAAGGACAAATGTGGGAGAACCCCATTGCACTATGCAGCTGCAAATTGCCATTTCCACTGTATTAA
Peptide sequence:
MDRNKMILGNAHDNSEELERAREVKEKDAALCLEFLLQNDANPSIRDKEGYNSIHYAAAYGHRQCLELQCRMHKALAEQRSRFSQKGQMWENPIALCSCKLPFPLY
IRESfinder result
IRESfinder score
0.296914623
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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