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mmu_circAnkrd44_2896
Basic information of circular RNA
PanCircBase ID:
mmu_circAnkrd44_2896
mm39_Chr. Position ID:
chr1|54690827|54867854|-
Gene name:
Ankrd44
Transcript:
ENSMUST00000179030.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
26
Exon/intron indexes:
27,26,25,24,23,22,21,20,19,18,17,16,15,14,13,12,11,10,9,8,7,6,5,4,3,2
Exon/intron offsets:
0,2074,3411,3992,5947,7250,9332,10985,12230,15703,29451,71345,75821,78065,81259,83327,84561,91645,101221,110592,111976,114681,115115,126618,140784,176943
Genomic length:
177027
Exon/intron sizes in nt:
92,83,83,146,84,88,217,118,88,202,72,112,81,141,69,64,83,115,79,213,143,88,201,71,79,84
Flanking exon/introns:
chr1:54687494-54690827|chr1:54867854-54965298
Splice length:
2896
Other database IDs:
circAtlas: hsa-SCAF8_0001
Human Ortholog IDs:
Mature spliced Sequence:
CCACCACTGGTCCAGGCAATCTTCAGTGGCGACCCAGAGGAGATTCGCATGCTCATCCATAAGACTGAAGACGTAAACGCACTGGATTCTGAGAAACGAACCCCGCTTCATGTGGCTGCATTCCTGGGAGATGCAGAGATCATTGAACTCCTGATTTTGTCAGGAGCTCGTGTAAATGCCAAGGACAACATGTGGCTGACCCCACTGCACCGGGCTGTTGCTTCCAGAAGTGAAGAGGCAGTGCAAGTATTGATTAAACACTCGGCTGATGTCAATGCAAGGGACAAGAATTGGCAGACCCCCCTCCATGTGGCAGCAGCCAATAAGGCCGTCAAGTGTGCAGAAGTGATCATTCCACTACTGAGCAGTGTCAATGTCTCCGACAGAGGCGGGCGCACAGCCTTGCACCACGCCGCTCTGAATGGACACATGGAGATGGTCAATTTACTCTTGGCCAAAGGGGCAAACATCAATGCATTTGACAAGAAGGACCGACGAGCTCTCCACTGGGCAGCATACATGGGTCACCTGGACGTTGTGGCGCTGCTCATTAACCATGGTGCAGAAGTTACCTGCAAGGATAAGAAGGGCTACACCCCGCTGCACGCTGCAGCCTCCAATGGGCAGATCAGTGTTGTCAAACATCTCCTGAACCTGGGGGTGGAGATTGATGAAATCAATGTCTATGGAAACACAGCACTTCACATTGCCTGCTACAATGGCCAGGATGCTGTCGTTAATGAGCTGATCGACTATGGTGCTAACGTGAATCAGCCCAACAACAGTGGCTTTACCCCTCTGCACTTTGCAGCGGCGTCTACTCACGGTGCTCTGTGTCTGGAGTTGTTAGTGAACAATGGGGCCGATGTGAACATTCAGAGTAAGGATGGCAAAAGTCCTCTGCACATGACAGCTGTCCATGGACGGTTCACGCGGTCACAAACCCTCATCCAGAATGGAGGTGAGATCGACTGTGTGGATAAGGATGGCAACACACCTCTCCATGTGGCTGCGAGACATGGTCACGAGCTCCTGATTAACACCCTAATCACTAGCGGAGCCGACACAGCCAAGTGTGGAATCCATAGCATGTTCCCCCTACATTTAGCCGCCCTAAATGCTCACTCTGACTGCTGCAGGAAGTTGTTATCATCTGGCTTTGAAATAGACACCCCAGATACGTTTGGAAGAACGTGCCTCCACGCCGCTGCTGCAGGAGGCAATGTAGAATGCATAAAGCTCTTGCAGAGCAGCGGAGCAGATTTTCACAAAAAGGACAAATGTGGGAGAACCCCATTGCACTATGCAGCTGCAAATTGCCATTTCCACTGTATTAAAGCATTAGTGACCACCGGAGCCAACGTTAATGAAACAGACGACTGGGGACGGACAGCTCTGCACTACGCTGCTGCATCAGACATGGATAGAAATAAGATGATCTTAGGAAATGCCCATGACAATTCTGAAGAACTTGAAAGAGCCAGAGAAGTGAAGGAAAAAGATGCTGCACTATGTCTAGAATTTCTGCTTCAAAATGATGCAAACCCATCCATCCGGGACAAGGAAGGCTACAATAGCATCCACTATGCTGCTGCCTATGGCCACAGACAGTGTCTAGAACTGCTTTTGGAAAGAACCAACACTGGCTTTGAAGAATCAGATGGCGGTGCTCTCAAGAGTCCACTCCACTTAGCAGCATACAATGGGCATCACCAAGCCTTGGAGGTCCTTCTCCAGTCCCTAGTGGACCTGGACATCAGGGATGAGAAAGGCCGGACAGCTCTGTACCTAGCTGCTTTCAAGGGACACACAGAATGTGTGGAGGCACTTGTTAACCAGGGCGCATCCATCTTTGTAAAGGACAATGTCACCAAAAGAACTCCACTTCATGCTTCAGTTATTAATGGTCACACACTGTGTTTGAGGCTACTACTTGAAACAGCAGACAATCCGGAAGTGGTTGATGTGAAAGATGCCAAGGGACAAACCCCGCTGATGCTTGCAGTAGCATATGGACACATTGATGCTGTTTCATTGTTACTTGAAAAGGAAGCAAACGTAGATGCGGTCGACATAGTGGGATGCACGGCTTTACATAGAGGGATTATGACAGGACATGAGGAATGTGTGCAAATGCTGCTGGAACAAGAAGCATCAATCCTCTGTAAAGATTCCAGAGGGAGGACACCCTTGCACTATGCGGCTGCTCGGGGCCACGCCACATGGCTGAATGAATTGCTCCAGATTGCCCTTTCTGAAGAAGACTGCTGTCTCAAAGACAACCAGGGATACACGCCACTGCACTGGGCGTGTTACAATGGTAATGAAAACTGCATAGAGGTACTTTTGGAGCAAAAATGTTTTCGAAAATTTATTGGTAATCCCTTCACTCCACTGCACTGTGCAATAATAAATGGTCACGAGAGCTGTGCATCATTGCTCCTGGGGGCCATAGATCCCAGCATTGTCAGCTGCAGGGATGACAAAGGCAGGACAACCCTCCACGCGGCAGCCTTTGGAGATCATGCGGAGTGCTTGCAGCTGCTTCTGAGACATGATGCTCAAGTGAATGCCGTAGATAATTCAGGGAAAACAGCACTGATGATGGCCGCTGAGAACGGGCAGGCAGGAGCTGTGGATATTTTGGTGAACAGTGCCCAGGCTGACCTGACTGTGAAGGATAAGGACTTGAATACTCCCTTACACCTGGCTATCAGTAAAGGCCATGAGAAATGTGCCTTGTTAATACTTGACAAGATACAAGATGAGAGCCTTATCAATGCGAAGAACAGTGCGCTGCAGACACCCCTCCATATCGCTGCACGCAATGGCTTGAAGGTGGTAGTTGAGGAGTTGCTGGCGAAAGGAGCCTGCGTACTTGCTGTAGATGAAAATG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GACACCCCTCCATATCGCTG
59.97
AGCATGCGAATCTCCTCTGG
59.89
148
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CAGACACCCCTCCATATCGC
59.97
AGCATGCGAATCTCCTCTGG
59.89
150
PCR template sequence:
CTGCAGACACCCCTCCATATCGCTGCACGCAATGGCTTGAAGGTGGTAGTTGAGGAGTTGCTGGCGAAAGGAGCCTGCGTACTTGCTGTAGATGAAAATGCCACCACTGGTCCAGGCAATCTTCAGTGGCGACCCAGAGGAGATTCGCATGCTCATCCATAAGACTGAAGACGTAAACGCACTGGATTCTGAGAAACGAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
37
Interacting miRNAs (#binding sites)
mmu-miR-298-5p(3), mmu-miR-103-3p(2), mmu-miR-211-5p(2), mmu-miR-322-5p(2), mmu-miR-149-5p(2), mmu-miR-301b-3p(2), mmu-miR-107-3p(2), mmu-miR-301a-3p(2), mmu-miR-670-5p(2), mmu-miR-155-5p(1), mmu-miR-16-5p(1), mmu-miR-15a-5p(1), mmu-miR-200a-3p(1), mmu-miR-141-3p(1), mmu-miR-20a-5p(1), mmu-miR-17-5p(1), mmu-miR-32-5p(1), mmu-miR-221-3p(1), mmu-miR-26a-5p(1), mmu-miR-204-5p(1), mmu-miR-106b-5p(1), mmu-miR-29b-3p(1), mmu-miR-29a-3p(1), mmu-miR-29c-3p(1), mmu-miR-33-5p(1), mmu-miR-130a-3p(1), mmu-miR-324-5p(1), mmu-miR-503-5p(1), mmu-miR-136-5p(1), mmu-miR-363-3p(1), mmu-miR-26b-5p(1), mmu-miR-130b-3p(1), mmu-miR-467b-5p(1), mmu-miR-758-3p(1), mmu-miR-680(1), mmu-miR-335-3p(1), mmu-miR-7a-2-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GTAGATGAAAATGCCACCACTGGTCC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCANKRD44_2896_ORF_1
1.141
0.212740057
0.999999992
2910
ORF sequence:
ATGCTCATCCATAAGACTGAAGACGTAAACGCACTGGATTCTGAGAAACGAACCCCGCTTCATGTGGCTGCATTCCTGGGAGATGCAGAGATCATTGAACTCCTGATTTTGTCAGGAGCTCGTGTAAATGCCAAGGACAACATGTGGCTGACCCCACTGCACCGGGCTGTTGCTTCCAGAAGTGAAGAGGCAGTGCAAGTATTGATTAAACACTCGGCTGATGTCAATGCAAGGGACAAGAATTGGCAGACCCCCCTCCATGTGGCAGCAGCCAATAAGGCCGTCAAGTGTGCAGAAGTGATCATTCCACTACTGAGCAGTGTCAATGTCTCCGACAGAGGCGGGCGCACAGCCTTGCACCACGCCGCTCTGAATGGACACATGGAGATGGTCAATTTACTCTTGGCCAAAGGGGCAAACATCAATGCATTTGACAAGAAGGACCGACGAGCTCTCCACTGGGCAGCATACATGGGTCACCTGGACGTTGTGGCGCTGCTCATTAACCATGGTGCAGAAGTTACCTGCAAGGATAAGAAGGGCTACACCCCGCTGCACGCTGCAGCCTCCAATGGGCAGATCAGTGTTGTCAAACATCTCCTGAACCTGGGGGTGGAGATTGATGAAATCAATGTCTATGGAAACACAGCACTTCACATTGCCTGCTACAATGGCCAGGATGCTGTCGTTAATGAGCTGATCGACTATGGTGCTAACGTGAATCAGCCCAACAACAGTGGCTTTACCCCTCTGCACTTTGCAGCGGCGTCTACTCACGGTGCTCTGTGTCTGGAGTTGTTAGTGAACAATGGGGCCGATGTGAACATTCAGAGTAAGGATGGCAAAAGTCCTCTGCACATGACAGCTGTCCATGGACGGTTCACGCGGTCACAAACCCTCATCCAGAATGGAGGTGAGATCGACTGTGTGGATAAGGATGGCAACACACCTCTCCATGTGGCTGCGAGACATGGTCACGAGCTCCTGATTAACACCCTAATCACTAGCGGAGCCGACACAGCCAAGTGTGGAATCCATAGCATGTTCCCCCTACATTTAGCCGCCCTAAATGCTCACTCTGACTGCTGCAGGAAGTTGTTATCATCTGGCTTTGAAATAGACACCCCAGATACGTTTGGAAGAACGTGCCTCCACGCCGCTGCTGCAGGAGGCAATGTAGAATGCATAAAGCTCTTGCAGAGCAGCGGAGCAGATTTTCACAAAAAGGACAAATGTGGGAGAACCCCATTGCACTATGCAGCTGCAAATTGCCATTTCCACTGTATTAAAGCATTAGTGACCACCGGAGCCAACGTTAATGAAACAGACGACTGGGGACGGACAGCTCTGCACTACGCTGCTGCATCAGACATGGATAGAAATAAGATGATCTTAGGAAATGCCCATGACAATTCTGAAGAACTTGAAAGAGCCAGAGAAGTGAAGGAAAAAGATGCTGCACTATGTCTAGAATTTCTGCTTCAAAATGATGCAAACCCATCCATCCGGGACAAGGAAGGCTACAATAGCATCCACTATGCTGCTGCCTATGGCCACAGACAGTGTCTAGAACTGCTTTTGGAAAGAACCAACACTGGCTTTGAAGAATCAGATGGCGGTGCTCTCAAGAGTCCACTCCACTTAGCAGCATACAATGGGCATCACCAAGCCTTGGAGGTCCTTCTCCAGTCCCTAGTGGACCTGGACATCAGGGATGAGAAAGGCCGGACAGCTCTGTACCTAGCTGCTTTCAAGGGACACACAGAATGTGTGGAGGCACTTGTTAACCAGGGCGCATCCATCTTTGTAAAGGACAATGTCACCAAAAGAACTCCACTTCATGCTTCAGTTATTAATGGTCACACACTGTGTTTGAGGCTACTACTTGAAACAGCAGACAATCCGGAAGTGGTTGATGTGAAAGATGCCAAGGGACAAACCCCGCTGATGCTTGCAGTAGCATATGGACACATTGATGCTGTTTCATTGTTACTTGAAAAGGAAGCAAACGTAGATGCGGTCGACATAGTGGGATGCACGGCTTTACATAGAGGGATTATGACAGGACATGAGGAATGTGTGCAAATGCTGCTGGAACAAGAAGCATCAATCCTCTGTAAAGATTCCAGAGGGAGGACACCCTTGCACTATGCGGCTGCTCGGGGCCACGCCACATGGCTGAATGAATTGCTCCAGATTGCCCTTTCTGAAGAAGACTGCTGTCTCAAAGACAACCAGGGATACACGCCACTGCACTGGGCGTGTTACAATGGTAATGAAAACTGCATAGAGGTACTTTTGGAGCAAAAATGTTTTCGAAAATTTATTGGTAATCCCTTCACTCCACTGCACTGTGCAATAATAAATGGTCACGAGAGCTGTGCATCATTGCTCCTGGGGGCCATAGATCCCAGCATTGTCAGCTGCAGGGATGACAAAGGCAGGACAACCCTCCACGCGGCAGCCTTTGGAGATCATGCGGAGTGCTTGCAGCTGCTTCTGAGACATGATGCTCAAGTGAATGCCGTAGATAATTCAGGGAAAACAGCACTGATGATGGCCGCTGAGAACGGGCAGGCAGGAGCTGTGGATATTTTGGTGAACAGTGCCCAGGCTGACCTGACTGTGAAGGATAAGGACTTGAATACTCCCTTACACCTGGCTATCAGTAAAGGCCATGAGAAATGTGCCTTGTTAATACTTGACAAGATACAAGATGAGAGCCTTATCAATGCGAAGAACAGTGCGCTGCAGACACCCCTCCATATCGCTGCACGCAATGGCTTGAAGGTGGTAGTTGAGGAGTTGCTGGCGAAAGGAGCCTGCGTACTTGCTGTAGATGAAAATGCCACCACTGGTCCAGGCAATCTTCAGTGGCGACCCAGAGGAGATTCGCATGCTCATCCATAA
Peptide sequence:
MLIHKTEDVNALDSEKRTPLHVAAFLGDAEIIELLILSGARVNAKDNMWLTPLHRAVASRSEEAVQVLIKHSADVNARDKNWQTPLHVAAANKAVKCAEVIIPLLSSVNVSDRGGRTALHHAALNGHMEMVNLLLAKGANINAFDKKDRRALHWAAYMGHLDVVALLINHGAEVTCKDKKGYTPLHAAASNGQISVVKHLLNLGVEIDEINVYGNTALHIACYNGQDAVVNELIDYGANVNQPNNSGFTPLHFAAASTHGALCLELLVNNGADVNIQSKDGKSPLHMTAVHGRFTRSQTLIQNGGEIDCVDKDGNTPLHVAARHGHELLINTLITSGADTAKCGIHSMFPLHLAALNAHSDCCRKLLSSGFEIDTPDTFGRTCLHAAAAGGNVECIKLLQSSGADFHKKDKCGRTPLHYAAANCHFHCIKALVTTGANVNETDDWGRTALHYAAASDMDRNKMILGNAHDNSEELERAREVKEKDAALCLEFLLQNDANPSIRDKEGYNSIHYAAAYGHRQCLELLLERTNTGFEESDGGALKSPLHLAAYNGHHQALEVLLQSLVDLDIRDEKGRTALYLAAFKGHTECVEALVNQGASIFVKDNVTKRTPLHASVINGHTLCLRLLLETADNPEVVDVKDAKGQTPLMLAVAYGHIDAVSLLLEKEANVDAVDIVGCTALHRGIMTGHEECVQMLLEQEASILCKDSRGRTPLHYAAARGHATWLNELLQIALSEEDCCLKDNQGYTPLHWACYNGNENCIEVLLEQKCFRKFIGNPFTPLHCAIINGHESCASLLLGAIDPSIVSCRDDKGRTTLHAAAFGDHAECLQLLLRHDAQVNAVDNSGKTALMMAAENGQAGAVDILVNSAQADLTVKDKDLNTPLHLAISKGHEKCALLILDKIQDESLINAKNSALQTPLHIAARNGLKVVVEELLAKGACVLAVDENATTGPGNLQWRPRGDSHAHP
IRESfinder result
IRESfinder score
0.387515718
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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