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mmu_circAnkrd17_957
Basic information of circular RNA
PanCircBase ID:
mmu_circAnkrd17_957
mm39_Chr. Position ID:
chr5|90435107|90443677|-
Gene name:
Ankrd17
Transcript:
ENSMUST00000014421.15
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
6
Exon/intron indexes:
11,10,9,8,7,6
Exon/intron offsets:
0,1335,1989,3699,5953,8336
Genomic length:
8570
Exon/intron sizes in nt:
8,81,10,19,22,38,95,230
Flanking exon/introns:
chr5:90433822-90435107|chr5:90443677-90445845
Splice length:
957
Other database IDs:
circAtlas: mmu-Ankrd17_0040
circAtlas: hsa-SCAF8_0001
Human Ortholog IDs:
Mature spliced Sequence:
GCAACACAGCTCTCACCTATGCTTGTGCTGGAGGCTACGTAGATGTTGTAAAGGTGCTCTTGGAATCCGGTGCTAGTATTGAGGACCATAATGAGAATGGTCACACACCTCTTATGGAAGCTGGAAGTGCTGGGCATGTGGAAGTAGCCAGATTGCTGCTAGAAAATGGAGCTGGCATCAATACGCATTCCAATGAATTTAAAGAGAGTGCCCTTACATTAGCTTGTTATAAAGGACATCTAGAGATGGTGCGATTTCTTTTGGAAGCAGGCGCTGATCAAGAACATAAGACAGATGAAATGCACACTGCTCTAATGGAGGCTTGCATGGATGGCCATGTTGAAGTAGCTAGGTTGCTTCTGGACAGTGGTGCTCAAGTGAACATGCCAGCTGATTCATTTGAGTCACCATTGACTTTGGCTGCATGTGGAGGACATGTGGAACTTGCAGCCTTACTTATTGAAAGAGGAGCTAGCCTGGAAGAGGTCAATGATGAAGGTTATACTCCTTTGATGGAGGCAGCTCGTGAAGGACATGAAGAAATGGTGGCGTTACTTCTTGGCCAAGGAGCAAATATCAATGCACAGACAGAAGAAACTCAAGAAACTGCCTTGACCCTGGCTTGCTGTGGAGGCTTTCTGGAAGTAGCAGACTTTCTGATTAAGGCTGGGGCTGATATAGAACTAGGGTGTTCTACTCCTTTGATGGAAGCTGCTCAAGAAGGTCACTTGGAGTTAGTGAAATACTTACTAGCTGCAGGAGCTAATGTTCATGCAACGACAGCAACAGGAGACACAGCTTTAACATATGCCTGTGAAAATGGCCATACGGATGTAGCAGACGTCTTACTCCAGGCAGGTGCAGATCTGGAACATGAGTCAGAAGGTGGAAGAACTCCTTTAATGAAAGCTGCAAGAGCGGGTCATGTTTGCACAGTCCAGTTCTTAATTAGCAAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGGAACATGAGTCAGAAGGTGG
59.96
CCGGATTCCAAGAGCACCTT
60.04
160
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGGAACATGAGTCAGAAGGTGG
59.96
TAGCACCGGATTCCAAGAGC
59.82
165
PCR template sequence:
GGTGCAGATCTGGAACATGAGTCAGAAGGTGGAAGAACTCCTTTAATGAAAGCTGCAAGAGCGGGTCATGTTTGCACAGTCCAGTTCTTAATTAGCAAAGGCAACACAGCTCTCACCTATGCTTGTGCTGGAGGCTACGTAGATGTTGTAAAGGTGCTCTTGGAATCCGGTGCTAGTATTGAGGACCATAATGAGAATGG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
8
Interacting miRNAs (#binding sites)
mmu-miR-15a-5p(1), mmu-miR-221-3p(1), mmu-miR-322-5p(1), mmu-miR-15b-5p(1), mmu-miR-33-5p(1), mmu-miR-222-3p(1), mmu-miR-22-3p(1), mmu-miR-335-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TTAATTAGCAAAGGCAACACAGCTCT
siRNA sets
AGAGCTGTGTTGCCTTTGC, GAGCTGTGTTGCCTTTGCT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCANKRD17_957_ORF_1
1.1802
0.091948476
0.999965422
1800
ORF sequence:
ATGGAAGCTGGAAGTGCTGGGCATGTGGAAGTAGCCAGATTGCTGCTAGAAAATGGAGCTGGCATCAATACGCATTCCAATGAATTTAAAGAGAGTGCCCTTACATTAGCTTGTTATAAAGGACATCTAGAGATGGTGCGATTTCTTTTGGAAGCAGGCGCTGATCAAGAACATAAGACAGATGAAATGCACACTGCTCTAATGGAGGCTTGCATGGATGGCCATGTTGAAGTAGCTAGGTTGCTTCTGGACAGTGGTGCTCAAGTGAACATGCCAGCTGATTCATTTGAGTCACCATTGACTTTGGCTGCATGTGGAGGACATGTGGAACTTGCAGCCTTACTTATTGAAAGAGGAGCTAGCCTGGAAGAGGTCAATGATGAAGGTTATACTCCTTTGATGGAGGCAGCTCGTGAAGGACATGAAGAAATGGTGGCGTTACTTCTTGGCCAAGGAGCAAATATCAATGCACAGACAGAAGAAACTCAAGAAACTGCCTTGACCCTGGCTTGCTGTGGAGGCTTTCTGGAAGTAGCAGACTTTCTGATTAAGGCTGGGGCTGATATAGAACTAGGGTGTTCTACTCCTTTGATGGAAGCTGCTCAAGAAGGTCACTTGGAGTTAGTGAAATACTTACTAGCTGCAGGAGCTAATGTTCATGCAACGACAGCAACAGGAGACACAGCTTTAACATATGCCTGTGAAAATGGCCATACGGATGTAGCAGACGTCTTACTCCAGGCAGGTGCAGATCTGGAACATGAGTCAGAAGGTGGAAGAACTCCTTTAATGAAAGCTGCAAGAGCGGGTCATGTTTGCACAGTCCAGTTCTTAATTAGCAAAGGCAACACAGCTCTCACCTATGCTTGTGCTGGAGGCTACGTAGATGTTGTAAAGGTGCTCTTGGAATCCGGTGCTAGTATTGAGGACCATAATGAGAATGGTCACACACCTCTTATGGAAGCTGGAAGTGCTGGGCATGTGGAAGTAGCCAGATTGCTGCTAGAAAATGGAGCTGGCATCAATACGCATTCCAATGAATTTAAAGAGAGTGCCCTTACATTAGCTTGTTATAAAGGACATCTAGAGATGGTGCGATTTCTTTTGGAAGCAGGCGCTGATCAAGAACATAAGACAGATGAAATGCACACTGCTCTAATGGAGGCTTGCATGGATGGCCATGTTGAAGTAGCTAGGTTGCTTCTGGACAGTGGTGCTCAAGTGAACATGCCAGCTGATTCATTTGAGTCACCATTGACTTTGGCTGCATGTGGAGGACATGTGGAACTTGCAGCCTTACTTATTGAAAGAGGAGCTAGCCTGGAAGAGGTCAATGATGAAGGTTATACTCCTTTGATGGAGGCAGCTCGTGAAGGACATGAAGAAATGGTGGCGTTACTTCTTGGCCAAGGAGCAAATATCAATGCACAGACAGAAGAAACTCAAGAAACTGCCTTGACCCTGGCTTGCTGTGGAGGCTTTCTGGAAGTAGCAGACTTTCTGATTAAGGCTGGGGCTGATATAGAACTAGGGTGTTCTACTCCTTTGATGGAAGCTGCTCAAGAAGGTCACTTGGAGTTAGTGAAATACTTACTAGCTGCAGGAGCTAATGTTCATGCAACGACAGCAACAGGAGACACAGCTTTAACATATGCCTGTGAAAATGGCCATACGGATGTAGCAGACGTCTTACTCCAGGCAGGTGCAGATCTGGAACATGAGTCAGAAGGTGGAAGAACTCCTTTAATGAAAGCTGCAAGAGCGGGTCATGTTTGCACAGTCCAGTTCTTAATTAGCAAA
Peptide sequence:
MEAGSAGHVEVARLLLENGAGINTHSNEFKESALTLACYKGHLEMVRFLLEAGADQEHKTDEMHTALMEACMDGHVEVARLLLDSGAQVNMPADSFESPLTLAACGGHVELAALLIERGASLEEVNDEGYTPLMEAAREGHEEMVALLLGQGANINAQTEETQETALTLACCGGFLEVADFLIKAGADIELGCSTPLMEAAQEGHLELVKYLLAAGANVHATTATGDTALTYACENGHTDVADVLLQAGADLEHESEGGRTPLMKAARAGHVCTVQFLISKGNTALTYACAGGYVDVVKVLLESGASIEDHNENGHTPLMEAGSAGHVEVARLLLENGAGINTHSNEFKESALTLACYKGHLEMVRFLLEAGADQEHKTDEMHTALMEACMDGHVEVARLLLDSGAQVNMPADSFESPLTLAACGGHVELAALLIERGASLEEVNDEGYTPLMEAAREGHEEMVALLLGQGANINAQTEETQETALTLACCGGFLEVADFLIKAGADIELGCSTPLMEAAQEGHLELVKYLLAAGANVHATTATGDTALTYACENGHTDVADVLLQAGADLEHESEGGRTPLMKAARAGHVCTVQFLISK
IRESfinder result
IRESfinder score
0.454771373
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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