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mmu_circAnkrd17_764
Basic information of circular RNA
PanCircBase ID:
mmu_circAnkrd17_764
mm39_Chr. Position ID:
chr5|90412485|90426250|-
Gene name:
Ankrd17
Transcript:
ENSMUST00000014421.15
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
20,19,18,17,16
Exon/intron offsets:
0,804,3705,4011,13616
Genomic length:
13765
Exon/intron sizes in nt:
21,41,43,14,61,12,149
Flanking exon/introns:
chr5:90412025-90412485|chr5:90426250-90430709
Splice length:
764
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
CAGTCAGTGGAAGAGCATCTGCAATGTCAAACACTCCTACCCACAGTATCGCTGCTTCTGTTTCCCAACCTCAGACTCCAACTCCAAGTCCCATCATCTCTCCTTCAGCCATGCTACCTATCTACCCTGCCATTGATATTGATGCACAGACTGAGAGTAATCATGATACTGCACTAACACTTGCCTGTGCTGGTGGCCATGAGGAACTGGTACAAACACTACTAGAGAGAGGAGCTAGTATTGAACATCGAGACAAGAAAGGTTTTACTCCACTCATCTTGGCTGCTACAGCTGGTCATGTTGGTGTTGTAGAGATATTGCTGGACAATGGTGCAGACATTGAAGCCCAGTCAGAAAGAACCAAGGACACACCACTGTCCTTGGCTTGTTCTGGGGGCAGGCAGGAGGTGGTGGAGTTGTTGTTAGCTCGCGGTGCAAATAAAGAACACAGGAATGTTTCTGATTATACACCTCTAAGCCTGGCTGCTTCTGGTGGCTATGTGAACATCATCAAAATACTGCTAAATGCAGGAGCTGAGATCAATTCAAGAACTGGCAGCAAATTGGGCATTTCTCCACTGATGTTGGCAGCTATGAATGGGCATACAGCTGCAGTTAAGCTCCTTTTAGACATGGGCTCTGACATAAATGCTCAGATAGAAACCAATCGGAACACTGCCCTTACATTAGCCTGCTTCCAAGGAAGAACTGAAGTGGTTAGCCTTCTGCTTGATCGAAAAGCAAATGTTGAACATAGGGCTAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGCCCTTACATTAGCCTGCT
59.08
TGGGAAACAGAAGCAGCGAT
59.96
155
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGCCCTTACATTAGCCTGCT
59.08
GTTGGGAAACAGAAGCAGCG
60.04
157
PCR template sequence:
CAATCGGAACACTGCCCTTACATTAGCCTGCTTCCAAGGAAGAACTGAAGTGGTTAGCCTTCTGCTTGATCGAAAAGCAAATGTTGAACATAGGGCTAAGCAGTCAGTGGAAGAGCATCTGCAATGTCAAACACTCCTACCCACAGTATCGCTGCTTCTGTTTCCCAACCTCAGACTCCAACTCCAAGTCCCATCATCTC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
7
Interacting miRNAs (#binding sites)
mmu-miR-34a-5p(1), mmu-miR-122-5p(1), mmu-miR-181b-5p(1), mmu-miR-433-3p(1), mmu-miR-22-3p(1), mmu-miR-27a-5p(1), mmu-miR-5101(1)
siRNAs for circular RNA silencing
Junction sequence
ACATAGGGCTAAGCAGTCAGTGGAAG
siRNA sets
CTTCCACTGACTGCTTAGC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCANKRD17_764_ORF_1
1.0125
0.047645994
0.968429873
918
ORF sequence:
ATGTCAAACACTCCTACCCACAGTATCGCTGCTTCTGTTTCCCAACCTCAGACTCCAACTCCAAGTCCCATCATCTCTCCTTCAGCCATGCTACCTATCTACCCTGCCATTGATATTGATGCACAGACTGAGAGTAATCATGATACTGCACTAACACTTGCCTGTGCTGGTGGCCATGAGGAACTGGTACAAACACTACTAGAGAGAGGAGCTAGTATTGAACATCGAGACAAGAAAGGTTTTACTCCACTCATCTTGGCTGCTACAGCTGGTCATGTTGGTGTTGTAGAGATATTGCTGGACAATGGTGCAGACATTGAAGCCCAGTCAGAAAGAACCAAGGACACACCACTGTCCTTGGCTTGTTCTGGGGGCAGGCAGGAGGTGGTGGAGTTGTTGTTAGCTCGCGGTGCAAATAAAGAACACAGGAATGTTTCTGATTATACACCTCTAAGCCTGGCTGCTTCTGGTGGCTATGTGAACATCATCAAAATACTGCTAAATGCAGGAGCTGAGATCAATTCAAGAACTGGCAGCAAATTGGGCATTTCTCCACTGATGTTGGCAGCTATGAATGGGCATACAGCTGCAGTTAAGCTCCTTTTAGACATGGGCTCTGACATAAATGCTCAGATAGAAACCAATCGGAACACTGCCCTTACATTAGCCTGCTTCCAAGGAAGAACTGAAGTGGTTAGCCTTCTGCTTGATCGAAAAGCAAATGTTGAACATAGGGCTAAGCAGTCAGTGGAAGAGCATCTGCAATGTCAAACACTCCTACCCACAGTATCGCTGCTTCTGTTTCCCAACCTCAGACTCCAACTCCAAGTCCCATCATCTCTCCTTCAGCCATGCTACCTATCTACCCTGCCATTGATATTGATGCACAGACTGAGAGTAATCATGATACTGCACTAA
Peptide sequence:
MSNTPTHSIAASVSQPQTPTPSPIISPSAMLPIYPAIDIDAQTESNHDTALTLACAGGHEELVQTLLERGASIEHRDKKGFTPLILAATAGHVGVVEILLDNGADIEAQSERTKDTPLSLACSGGRQEVVELLLARGANKEHRNVSDYTPLSLAASGGYVNIIKILLNAGAEINSRTGSKLGISPLMLAAMNGHTAAVKLLLDMGSDINAQIETNRNTALTLACFQGRTEVVSLLLDRKANVEHRAKQSVEEHLQCQTLLPTVSLLLFPNLRLQLQVPSSLLQPCYLSTLPLILMHRLRVIMILH
IRESfinder result
IRESfinder score
0.610428848
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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