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mmu_circAnkrd12_708
Basic information of circular RNA
PanCircBase ID:
mmu_circAnkrd12_708
mm39_Chr. Position ID:
chr17|66334246|66356928|-
Gene name:
Ankrd12
Transcript:
ENSMUST00000038116.13
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
7,6,5,4,3
Exon/intron offsets:
0,4119,10241,15281,22534
Genomic length:
22682
Exon/intron sizes in nt:
1,43,20,11,47,69,148
Flanking exon/introns:
chr17:66331218-66334246|chr17:66356928-66359948
Splice length:
708
Other database IDs:
CIRCpedia: MMU_CIRCpedia_214622, circAtlas: mmu-Ankrd12_0037
circAtlas: hsa-ANKRD12_0009, CIRCpedia: HSA_CIRCpedia_368208
Human Ortholog IDs:
Mature spliced Sequence:
AGTAAAGACAAGATTGCATCTTACAGCAAAACTTCAAAAATTGACCGAGGTGATATGGGCAAGGAAATGAGAGAGAAACCATCCATGAAACGAAAGCTGCCTTTTACAGTCAGCCCATCGAGAAATGAGGAGCGCGACTCAGACACAGATTCAGACCCAGGACATACAAGTGAAAATTGGGGGGAGAGACTTATATCTTCTTACAGGACATACTCAGAAAAAGAAGGTCCAGAGAAGAAGAAAACAAAAAAAGAAGCTGGAACTAAGAAGTCCACCCCAGTTAGCATTCTTTTTGGTTATCCACTTTCTGAACGAAAGCAGATGGCACTCCTAATGCAGATGACAGCAAGGGACAACAGCCCAGATTCCACACCAAGTCATCCGTCACAGGCAACGCCAGCCCAGAAGAAAACGCCCAGCTCTTCATCTCGACAGAAAGATAAAATTAATAAAAGAAATGAGCGTGGCGAAACTCCTTTACACATGGCCGCTATTCGAGGAGACGTGAAGCAAGTCAAAGAGTTAATTAGTTTAGGGGCAAACGTCAATGTGAAGGACTTTGCAGGTTGGACCCCTTTGCATGAAGCTTGCAATGTTGGCTATTACGATGTTGCTAAGATACTGATAGCAGCTGGCGCAGACGTTAACACACAAGGACTGGATGATGACACCCCGCTCCATGACTCTGCCAGCAGTGGCCATAGAGAC
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCGCAGACGTTAACACACAA
59.7
TTCCTTGCCCATATCACCTCG
59.86
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CTGGCGCAGACGTTAACACA
61.22
CCTTGCCCATATCACCTCGG
60.25
141
PCR template sequence:
TGTTGCTAAGATACTGATAGCAGCTGGCGCAGACGTTAACACACAAGGACTGGATGATGACACCCCGCTCCATGACTCTGCCAGCAGTGGCCATAGAGACAGTAAAGACAAGATTGCATCTTACAGCAAAACTTCAAAAATTGACCGAGGTGATATGGGCAAGGAAATGAGAGAGAAACCATCCATGAAACGAAAGCTGC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
7
Interacting miRNAs (#binding sites)
mmu-miR-103-3p(1), mmu-miR-143-3p(1), mmu-miR-297a-5p(1), mmu-miR-107-3p(1), mmu-miR-342-5p(1), mmu-miR-669d-5p(1), mmu-miR-1199-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TGGCCATAGAGACAGTAAAGACAAGA
siRNA sets
TCTTTACTGTCTCTATGGC,TTTACTGTCTCTATGGCCA,GTCTTTACTGTCTCTATGG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCANKRD12_708_ORF_1
0.9764
0.179380079
0.999176886
1362
ORF sequence:
ATGGGCAAGGAAATGAGAGAGAAACCATCCATGAAACGAAAGCTGCCTTTTACAGTCAGCCCATCGAGAAATGAGGAGCGCGACTCAGACACAGATTCAGACCCAGGACATACAAGTGAAAATTGGGGGGAGAGACTTATATCTTCTTACAGGACATACTCAGAAAAAGAAGGTCCAGAGAAGAAGAAAACAAAAAAAGAAGCTGGAACTAAGAAGTCCACCCCAGTTAGCATTCTTTTTGGTTATCCACTTTCTGAACGAAAGCAGATGGCACTCCTAATGCAGATGACAGCAAGGGACAACAGCCCAGATTCCACACCAAGTCATCCGTCACAGGCAACGCCAGCCCAGAAGAAAACGCCCAGCTCTTCATCTCGACAGAAAGATAAAATTAATAAAAGAAATGAGCGTGGCGAAACTCCTTTACACATGGCCGCTATTCGAGGAGACGTGAAGCAAGTCAAAGAGTTAATTAGTTTAGGGGCAAACGTCAATGTGAAGGACTTTGCAGGTTGGACCCCTTTGCATGAAGCTTGCAATGTTGGCTATTACGATGTTGCTAAGATACTGATAGCAGCTGGCGCAGACGTTAACACACAAGGACTGGATGATGACACCCCGCTCCATGACTCTGCCAGCAGTGGCCATAGAGACAGTAAAGACAAGATTGCATCTTACAGCAAAACTTCAAAAATTGACCGAGGTGATATGGGCAAGGAAATGAGAGAGAAACCATCCATGAAACGAAAGCTGCCTTTTACAGTCAGCCCATCGAGAAATGAGGAGCGCGACTCAGACACAGATTCAGACCCAGGACATACAAGTGAAAATTGGGGGGAGAGACTTATATCTTCTTACAGGACATACTCAGAAAAAGAAGGTCCAGAGAAGAAGAAAACAAAAAAAGAAGCTGGAACTAAGAAGTCCACCCCAGTTAGCATTCTTTTTGGTTATCCACTTTCTGAACGAAAGCAGATGGCACTCCTAATGCAGATGACAGCAAGGGACAACAGCCCAGATTCCACACCAAGTCATCCGTCACAGGCAACGCCAGCCCAGAAGAAAACGCCCAGCTCTTCATCTCGACAGAAAGATAAAATTAATAAAAGAAATGAGCGTGGCGAAACTCCTTTACACATGGCCGCTATTCGAGGAGACGTGAAGCAAGTCAAAGAGTTAATTAGTTTAGGGGCAAACGTCAATGTGAAGGACTTTGCAGGTTGGACCCCTTTGCATGAAGCTTGCAATGTTGGCTATTACGATGTTGCTAAGATACTGATAGCAGCTGGCGCAGACGTTAACACACAAGGACTGGATGATGACACCCCGCTCCATGACTCTGCCAGCAGTGGCCATAGAGAC
Peptide sequence:
MGKEMREKPSMKRKLPFTVSPSRNEERDSDTDSDPGHTSENWGERLISSYRTYSEKEGPEKKKTKKEAGTKKSTPVSILFGYPLSERKQMALLMQMTARDNSPDSTPSHPSQATPAQKKTPSSSSRQKDKINKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDSKDKIASYSKTSKIDRGDMGKEMREKPSMKRKLPFTVSPSRNEERDSDTDSDPGHTSENWGERLISSYRTYSEKEGPEKKKTKKEAGTKKSTPVSILFGYPLSERKQMALLMQMTARDNSPDSTPSHPSQATPAQKKTPSSSSRQKDKINKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRD
IRESfinder result
IRESfinder score
0.359672512
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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