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mmu_circAnkrd12_491
Basic information of circular RNA
PanCircBase ID:
mmu_circAnkrd12_491
mm39_Chr. Position ID:
chr17|66334246|66344634|-
Gene name:
Ankrd12
Transcript:
ENSMUST00000038116.13
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
7,6,5
Exon/intron offsets:
0,4119,10241
Genomic length:
10388
Exon/intron sizes in nt:
14,32,01,147
Flanking exon/introns:
chr17:66331218-66334246|chr17:66344634-66349527
Splice length:
491
Other database IDs:
circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_358544
Human Ortholog IDs:
Mature spliced Sequence:
AAAAAGAAGGTCCAGAGAAGAAGAAAACAAAAAAAGAAGCTGGAACTAAGAAGTCCACCCCAGTTAGCATTCTTTTTGGTTATCCACTTTCTGAACGAAAGCAGATGGCACTCCTAATGCAGATGACAGCAAGGGACAACAGCCCAGATTCCACACCAAGTCATCCGTCACAGGCAACGCCAGCCCAGAAGAAAACGCCCAGCTCTTCATCTCGACAGAAAGATAAAATTAATAAAAGAAATGAGCGTGGCGAAACTCCTTTACACATGGCCGCTATTCGAGGAGACGTGAAGCAAGTCAAAGAGTTAATTAGTTTAGGGGCAAACGTCAATGTGAAGGACTTTGCAGGTTGGACCCCTTTGCATGAAGCTTGCAATGTTGGCTATTACGATGTTGCTAAGATACTGATAGCAGCTGGCGCAGACGTTAACACACAAGGACTGGATGATGACACCCCGCTCCATGACTCTGCCAGCAGTGGCCATAGAGAC
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCGCAGACGTTAACACACAA
59.7
TGCTAACTGGGGTGGACTTC
59.31
143
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GCGCAGACGTTAACACACAA
59.7
GCTAACTGGGGTGGACTTCT
59.02
142
PCR template sequence:
TGTTGCTAAGATACTGATAGCAGCTGGCGCAGACGTTAACACACAAGGACTGGATGATGACACCCCGCTCCATGACTCTGCCAGCAGTGGCCATAGAGACAAAAAGAAGGTCCAGAGAAGAAGAAAACAAAAAAAGAAGCTGGAACTAAGAAGTCCACCCCAGTTAGCATTCTTTTTGGTTATCCACTTTCTGAACGAAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-103-3p(1), mmu-miR-143-3p(1), mmu-miR-107-3p(1), mmu-miR-342-5p(1), mmu-miR-669d-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TGGCCATAGAGACAAAAAGAAGGTCC
siRNA sets
TTCTTTTTGTCTCTATGGC, TCTTTTTGTCTCTATGGCC, CTTCTTTTTGTCTCTATGG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCANKRD12_491_ORF_1
0.8239
0.133573263
0.610456077
504
ORF sequence:
ATGGCACTCCTAATGCAGATGACAGCAAGGGACAACAGCCCAGATTCCACACCAAGTCATCCGTCACAGGCAACGCCAGCCCAGAAGAAAACGCCCAGCTCTTCATCTCGACAGAAAGATAAAATTAATAAAAGAAATGAGCGTGGCGAAACTCCTTTACACATGGCCGCTATTCGAGGAGACGTGAAGCAAGTCAAAGAGTTAATTAGTTTAGGGGCAAACGTCAATGTGAAGGACTTTGCAGGTTGGACCCCTTTGCATGAAGCTTGCAATGTTGGCTATTACGATGTTGCTAAGATACTGATAGCAGCTGGCGCAGACGTTAACACACAAGGACTGGATGATGACACCCCGCTCCATGACTCTGCCAGCAGTGGCCATAGAGACAAAAAGAAGGTCCAGAGAAGAAGAAAACAAAAAAAGAAGCTGGAACTAAGAAGTCCACCCCAGTTAGCATTCTTTTTGGTTATCCACTTTCTGAACGAAAGCAGATGGCACTCCTAA
Peptide sequence:
MALLMQMTARDNSPDSTPSHPSQATPAQKKTPSSSSRQKDKINKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDKKKVQRRRKQKKKLELRSPPQLAFFLVIHFLNESRWHS
IRESfinder result
IRESfinder score
0.274511984
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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