top of page
mmu_circAnkrd12_344
Basic information of circular RNA
PanCircBase ID:
mmu_circAnkrd12_344
mm39_Chr. Position ID:
chr17|66334246|66338566|-
Gene name:
Ankrd12
Transcript:
ENSMUST00000038116.13
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
7,6
Exon/intron offsets:
0,4119
Genomic length:
4320
Exon/intron sizes in nt:
1,43,201
Flanking exon/introns:
chr17:66331218-66334246|chr17:66338566-66344487
Splice length:
344
Other database IDs:
circBase: hsa_circ_0046848, circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_24458
Human Ortholog IDs:
Mature spliced Sequence:
ATTCCACACCAAGTCATCCGTCACAGGCAACGCCAGCCCAGAAGAAAACGCCCAGCTCTTCATCTCGACAGAAAGATAAAATTAATAAAAGAAATGAGCGTGGCGAAACTCCTTTACACATGGCCGCTATTCGAGGAGACGTGAAGCAAGTCAAAGAGTTAATTAGTTTAGGGGCAAACGTCAATGTGAAGGACTTTGCAGGTTGGACCCCTTTGCATGAAGCTTGCAATGTTGGCTATTACGATGTTGCTAAGATACTGATAGCAGCTGGCGCAGACGTTAACACACAAGGACTGGATGATGACACCCCGCTCCATGACTCTGCCAGCAGTGGCCATAGAGAC
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCGCAGACGTTAACACACAA
59.7
GAGATGAAGAGCTGGGCGTT
60.11
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GCGCAGACGTTAACACACAA
59.7
GTCGAGATGAAGAGCTGGGC
60.53
143
PCR template sequence:
TGTTGCTAAGATACTGATAGCAGCTGGCGCAGACGTTAACACACAAGGACTGGATGATGACACCCCGCTCCATGACTCTGCCAGCAGTGGCCATAGAGACATTCCACACCAAGTCATCCGTCACAGGCAACGCCAGCCCAGAAGAAAACGCCCAGCTCTTCATCTCGACAGAAAGATAAAATTAATAAAAGAAATGAGCG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-103-3p(1), mmu-miR-143-3p(1), mmu-miR-107-3p(1), mmu-miR-669d-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TGGCCATAGAGACATTCCACACCAAG
siRNA sets
CTTGGTGTGGAATGTCTCT, TTGGTGTGGAATGTCTCTA, TGGAATGTCTCTATGGCCA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCANKRD12_344_ORF_1
1.0186
0.124063295
0.451429343
369
ORF sequence:
ATGGCCGCTATTCGAGGAGACGTGAAGCAAGTCAAAGAGTTAATTAGTTTAGGGGCAAACGTCAATGTGAAGGACTTTGCAGGTTGGACCCCTTTGCATGAAGCTTGCAATGTTGGCTATTACGATGTTGCTAAGATACTGATAGCAGCTGGCGCAGACGTTAACACACAAGGACTGGATGATGACACCCCGCTCCATGACTCTGCCAGCAGTGGCCATAGAGACATTCCACACCAAGTCATCCGTCACAGGCAACGCCAGCCCAGAAGAAAACGCCCAGCTCTTCATCTCGACAGAAAGATAAAATTAATAAAAGAAATGAGCGTGGCGAAACTCCTTTACACATGGCCGCTATTCGAGGAGACGTGA
Peptide sequence:
MAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIPHQVIRHRQRQPRRKRPALHLDRKIKLIKEMSVAKLLYTWPLFEET
IRESfinder result
IRESfinder score
0.252384158
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page