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mmu_circAnkhd1_1322
Basic information of circular RNA
PanCircBase ID:
mmu_circAnkhd1_1322
mm39_Chr. Position ID:
chr18|36711670|36733928|+
Gene name:
Ankhd1
Transcript:
ENSMUST00000006205.14
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
8
Exon/intron indexes:
3,4,5,6,7,8,9,10
Exon/intron offsets:
0,2450,4994,10730,12829,15467,16184,22148
Genomic length:
22258
Exon/intron sizes in nt:
15,71,48,14,82,34,95,20,00,00,000
Flanking exon/introns:
chr18:36708222-36711670|chr18:36733928-36744852
Splice length:
1322
Other database IDs:
circAtlas: mmu-Ankhd1_0021
circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_50502
Human Ortholog IDs:
Mature spliced Sequence:
GAATTGGCAAATTGTCAACTGCTGATGGTAAAGCTTTTGCAGATCCTGAAGTACTCCGGAGACTGACATCCTCAGTTAGCTGTGCCCTGGATGAAGCCGCTGCTGCACTGACACGGATGAGAGCAGAGAACACACACAGTACAGGACAAGTGGATACTCGCAGTCTGGCAGAAGCTTGTTCAGATGGAGATGTGAATGCTGTTCGTAAATTACTAGATGAAGGCAGAAGTGTAAATGAACATACAGAAGAAGGAGAAAGCCTGCTGTGTTTGGCTTGTTCAGCAGGATATTATGAACTAGCACAAGTACTACTAGCTATGCATGCTAATGTTGAAGATAGAGGGAATAAAGGAGATATAACTCCTTTGATGGCAGCTTCTAGTGGAGGTTATTTAGATATTGTGAAATTATTACTTCTTCATGATGCAGATGTCAACTCCCAGTCGGCAACAGGGAACACTGCACTGACTTATGCTTGTGCTGGAGGATTTATTGACATTGTAAAAGTGCTTCTTAATGAGGGTGCGAATATAGAAGACCATAATGAAAATGGACACACTCCCCTAATGGAAGCAGCCAGTGCCGGTCATGTAGAAGTTGCCAGAGTTCTTCTAGATCACGGTGCAGGCATCAACACTCATTCTAATGAGTTTAAAGAAAGTGCTCTGACACTTGCCTGCTACAAAGGCCATTTGGATATGGTTCGATTTTTGCTTGAAGCTGGTGCAGATCAAGAGCACAAAACCGATGAGATGCACACTGCCTTAATGGAAGCTTGCATGGATGGACATGTAGAGGTGGCACGTTTGCTTTTAGACAGTGGTGCTCAGGTGAATATGCCTGCGGATTCCTTTGAGTCTCCATTGACTCTAGCTGCCTGTGGTGGACATGTTGAATTAGCAGCACTGCTTATTGAAAGAGGAGCAAATCTTGAAGAAGTTAATGACGAAGGATACACGCCTTTGATGGAAGCTGCTCGAGAAGGACATGAAGAGATGGTGGCACTTCTATTGGCACAAGGTGCAAATATAAATGCCCAGACAGAAGAAACTCAAGAAACTGCTCTTACCTTGGCTTGCTGTGGAGGCTTTTCTGAAGTTGCAGATTTCTTGATTAAGGCAGGGGCTGACATAGAACTTGGCTGTTCTACACCTCTCATGGAGGCTTCTCAGGAGGGACACCTGGAGTTGGTTAAATATTTGTTGGCTGCTGGTGCTAATGTGCATGCTACCACAGCTACAGGAGACACAGCTCTAACCTATGCTTGTGAAAATGGACATACAGATGTTGCAGATGTTCTACTTCAAGCAGGGGCCCATTTA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACAGCTACAGGAGACACAGC
59.39
TGAGGATGTCAGTCTCCGGA
59.67
164
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACAGCTACAGGAGACACAGC
59.39
AGGATGTCAGTCTCCGGAGT
59.67
162
PCR template sequence:
GCATGCTACCACAGCTACAGGAGACACAGCTCTAACCTATGCTTGTGAAAATGGACATACAGATGTTGCAGATGTTCTACTTCAAGCAGGGGCCCATTTAGAATTGGCAAATTGTCAACTGCTGATGGTAAAGCTTTTGCAGATCCTGAAGTACTCCGGAGACTGACATCCTCAGTTAGCTGTGCCCTGGATGAAGCCGC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
14
Interacting miRNAs (#binding sites)
mmu-miR-34c-5p(1), mmu-miR-34a-5p(1), mmu-miR-122-5p(1), mmu-miR-29b-3p(1), mmu-miR-29a-3p(1), mmu-miR-29c-3p(1), mmu-miR-148b-3p(1), mmu-miR-351-5p(1), mmu-miR-22-3p(1), mmu-miR-34b-5p(1), mmu-miR-297a-5p(1), mmu-miR-335-3p(1), mmu-miR-574-5p(1), mmu-miR-344-3p(1)
siRNAs for circular RNA silencing
Junction sequence
AGGGGCCCATTTAGAATTGGCAAATT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCANKHD1_1322_ORF_1
1.1941
0.059466324
0.998235331
1272
ORF sequence:
ATGAGAGCAGAGAACACACACAGTACAGGACAAGTGGATACTCGCAGTCTGGCAGAAGCTTGTTCAGATGGAGATGTGAATGCTGTTCGTAAATTACTAGATGAAGGCAGAAGTGTAAATGAACATACAGAAGAAGGAGAAAGCCTGCTGTGTTTGGCTTGTTCAGCAGGATATTATGAACTAGCACAAGTACTACTAGCTATGCATGCTAATGTTGAAGATAGAGGGAATAAAGGAGATATAACTCCTTTGATGGCAGCTTCTAGTGGAGGTTATTTAGATATTGTGAAATTATTACTTCTTCATGATGCAGATGTCAACTCCCAGTCGGCAACAGGGAACACTGCACTGACTTATGCTTGTGCTGGAGGATTTATTGACATTGTAAAAGTGCTTCTTAATGAGGGTGCGAATATAGAAGACCATAATGAAAATGGACACACTCCCCTAATGGAAGCAGCCAGTGCCGGTCATGTAGAAGTTGCCAGAGTTCTTCTAGATCACGGTGCAGGCATCAACACTCATTCTAATGAGTTTAAAGAAAGTGCTCTGACACTTGCCTGCTACAAAGGCCATTTGGATATGGTTCGATTTTTGCTTGAAGCTGGTGCAGATCAAGAGCACAAAACCGATGAGATGCACACTGCCTTAATGGAAGCTTGCATGGATGGACATGTAGAGGTGGCACGTTTGCTTTTAGACAGTGGTGCTCAGGTGAATATGCCTGCGGATTCCTTTGAGTCTCCATTGACTCTAGCTGCCTGTGGTGGACATGTTGAATTAGCAGCACTGCTTATTGAAAGAGGAGCAAATCTTGAAGAAGTTAATGACGAAGGATACACGCCTTTGATGGAAGCTGCTCGAGAAGGACATGAAGAGATGGTGGCACTTCTATTGGCACAAGGTGCAAATATAAATGCCCAGACAGAAGAAACTCAAGAAACTGCTCTTACCTTGGCTTGCTGTGGAGGCTTTTCTGAAGTTGCAGATTTCTTGATTAAGGCAGGGGCTGACATAGAACTTGGCTGTTCTACACCTCTCATGGAGGCTTCTCAGGAGGGACACCTGGAGTTGGTTAAATATTTGTTGGCTGCTGGTGCTAATGTGCATGCTACCACAGCTACAGGAGACACAGCTCTAACCTATGCTTGTGAAAATGGACATACAGATGTTGCAGATGTTCTACTTCAAGCAGGGGCCCATTTAGAATTGGCAAATTGTCAACTGCTGATGGTAAAGCTTTTGCAGATCCTGAAGTACTCCGGAGACTGA
Peptide sequence:
MRAENTHSTGQVDTRSLAEACSDGDVNAVRKLLDEGRSVNEHTEEGESLLCLACSAGYYELAQVLLAMHANVEDRGNKGDITPLMAASSGGYLDIVKLLLLHDADVNSQSATGNTALTYACAGGFIDIVKVLLNEGANIEDHNENGHTPLMEAASAGHVEVARVLLDHGAGINTHSNEFKESALTLACYKGHLDMVRFLLEAGADQEHKTDEMHTALMEACMDGHVEVARLLLDSGAQVNMPADSFESPLTLAACGGHVELAALLIERGANLEEVNDEGYTPLMEAAREGHEEMVALLLAQGANINAQTEETQETALTLACCGGFSEVADFLIKAGADIELGCSTPLMEASQEGHLELVKYLLAAGANVHATTATGDTALTYACENGHTDVADVLLQAGAHLELANCQLLMVKLLQILKYSGD
IRESfinder result
IRESfinder score
0.517620579
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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