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mmu_circAnkhd1_1310
Basic information of circular RNA
PanCircBase ID:
mmu_circAnkhd1_1310
mm39_Chr. Position ID:
chr18|36771257|36778292|+
Gene name:
Ankhd1
Transcript:
ENSMUST00000006205.14
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
21,22,23,24,25,26,27
Exon/intron offsets:
0,341,2060,3372,4721,6056,6900
Genomic length:
7035
Exon/intron sizes in nt:
1,76,16,39,61,17,16,94,00,000
Flanking exon/introns:
chr18:36767858-36771257|chr18:36778292-36779743
Splice length:
1310
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
ACTGGTCTTACACCCTTGATGGAAGCAGCTTCTGGAGGATATGCAGAGGTTGGAAGAGTTCTCCTTGATAAAGGAGCAGATGTCAATGCACCTCCTGTGCCTTCCTCAAGAGACACTGCTTTAACCATAGCAGCAGACAAAGGCCACTACAAATTCTGTGAGCTCCTGATTAACAGGGGAGCACATATTGATGTTCGTAACAAGAAGGGAAATACACCACTTTGGCTGGCATCCAATGGTGGTCATTTTGATGTAGTACAATTGCTAGTGCAAGCAGGTGCTGATGTGGATGCAGCAGACAACCGAAAAATCACACCTCTTATGTCAGCATTTCGCAAGGGTCATGTAAAAGTTGTTCAGTATTTGGTGAAGGAAGTCAGTCAGTTTCCATCTGATATAGAATGCATGAGATACATAGCAACAATTACAGATAAGGAACTGCTGAAGAAGTGTCACCAATGTGTTGAGACAATTGTGAAGGCTAAAGATCAGCAGGCTGCAGAAGCAAATAAAAATGCAAGCATTCTCCTAAAAGAGCTGGATCTAGAAAAGTCAAGAGAAGAGAGCAGAAAGCAAGCTCTTGCTGCTAAAAGAGAGAAGCGAAAAGAAAAGAGGAAAAAGAAAAAAGAGGAACAGAAAAGGAAACAGGAAGATGAGGAGAACAAACCTAAGGAGAATTCAGAACAACCAGAGGGTGAAGATGAAGAGAATGATGAAGATGTGGAGCAAGAAATTCCCATAGAGCCTCCGAGTGCAACCACCACTACTACAATTGGAATCTCTGCTACATCTACCACGTTTACAAATGTATTTGGCAAAAAAAGAGCCAATGTAGTGACAACCCCCAGCACCAATCGGAAAAATAAGAAGAATAAGACAAAAGAGTCCCCTCCCACAGCGCATTTAATTTTACCTGAGCCACATATATCTTTAGCACAACAAAAAGCAGATAAAAATAAAATAAATGGAGAGCCTAGAGGTGGCGGCGCAGGTGGAAATAGTGACTCGGATAACATAGATAGCACAGACTGCAACAGTGAGAGCAGCAGTGGTGGGAAGAGCCAAGAGTTCAGTTTCCCTGTGGATGTGAATCCCGCCAGTGACAAGAGGTGCTCCACAGTTGTCAGTTCTCAAGAGGAAAAGGCAGTTACCACCACTTCCAAGACTCAGACACGCCTTGATGGTGAAGTAAATTCCATGTCAACAAGCTACAAATCTTTGCCATTAAGTTCTCCAACCATGAAACTGAATCTCACTAGTCCTAAAAGGGGTCAAAAAAGAGAAGAAGGGTGGAAAGAAGTTGTTAGAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CTCACTAGTCCTAAAAGGGGTCA
58.91
GGTGCATTGACATCTGCTCC
59.26
151
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CTCACTAGTCCTAAAAGGGGTCA
58.91
GGAGGTGCATTGACATCTGC
59.26
154
PCR template sequence:
ACAAATCTTTGCCATTAAGTTCTCCAACCATGAAACTGAATCTCACTAGTCCTAAAAGGGGTCAAAAAAGAGAAGAAGGGTGGAAAGAAGTTGTTAGAAGACTGGTCTTACACCCTTGATGGAAGCAGCTTCTGGAGGATATGCAGAGGTTGGAAGAGTTCTCCTTGATAAAGGAGCAGATGTCAATGCACCTCCTGTGC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
13
Interacting miRNAs (#binding sites)
mmu-miR-146a-5p(2), mmu-miR-146b-5p(2), mmu-miR-124-3p(1), mmu-miR-322-5p(1), mmu-miR-15b-5p(1), mmu-miR-33-5p(1), mmu-miR-217-5p(1), mmu-miR-136-5p(1), mmu-miR-370-3p(1), mmu-miR-541-5p(1), mmu-miR-335-3p(1), mmu-miR-1904(1), mmu-miR-544-5p(1)
siRNAs for circular RNA silencing
Junction sequence
AAGTTGTTAGAAGACTGGTCTTACAC
siRNA sets
AAGACCAGTCTTCTAACAA, GTAAGACCAGTCTTCTAAC, TAAGACCAGTCTTCTAACA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCANKHD1_1310_ORF_1
1.0596
0.122439557
0.998678092
1311
ORF sequence:
ATGGAAGCAGCTTCTGGAGGATATGCAGAGGTTGGAAGAGTTCTCCTTGATAAAGGAGCAGATGTCAATGCACCTCCTGTGCCTTCCTCAAGAGACACTGCTTTAACCATAGCAGCAGACAAAGGCCACTACAAATTCTGTGAGCTCCTGATTAACAGGGGAGCACATATTGATGTTCGTAACAAGAAGGGAAATACACCACTTTGGCTGGCATCCAATGGTGGTCATTTTGATGTAGTACAATTGCTAGTGCAAGCAGGTGCTGATGTGGATGCAGCAGACAACCGAAAAATCACACCTCTTATGTCAGCATTTCGCAAGGGTCATGTAAAAGTTGTTCAGTATTTGGTGAAGGAAGTCAGTCAGTTTCCATCTGATATAGAATGCATGAGATACATAGCAACAATTACAGATAAGGAACTGCTGAAGAAGTGTCACCAATGTGTTGAGACAATTGTGAAGGCTAAAGATCAGCAGGCTGCAGAAGCAAATAAAAATGCAAGCATTCTCCTAAAAGAGCTGGATCTAGAAAAGTCAAGAGAAGAGAGCAGAAAGCAAGCTCTTGCTGCTAAAAGAGAGAAGCGAAAAGAAAAGAGGAAAAAGAAAAAAGAGGAACAGAAAAGGAAACAGGAAGATGAGGAGAACAAACCTAAGGAGAATTCAGAACAACCAGAGGGTGAAGATGAAGAGAATGATGAAGATGTGGAGCAAGAAATTCCCATAGAGCCTCCGAGTGCAACCACCACTACTACAATTGGAATCTCTGCTACATCTACCACGTTTACAAATGTATTTGGCAAAAAAAGAGCCAATGTAGTGACAACCCCCAGCACCAATCGGAAAAATAAGAAGAATAAGACAAAAGAGTCCCCTCCCACAGCGCATTTAATTTTACCTGAGCCACATATATCTTTAGCACAACAAAAAGCAGATAAAAATAAAATAAATGGAGAGCCTAGAGGTGGCGGCGCAGGTGGAAATAGTGACTCGGATAACATAGATAGCACAGACTGCAACAGTGAGAGCAGCAGTGGTGGGAAGAGCCAAGAGTTCAGTTTCCCTGTGGATGTGAATCCCGCCAGTGACAAGAGGTGCTCCACAGTTGTCAGTTCTCAAGAGGAAAAGGCAGTTACCACCACTTCCAAGACTCAGACACGCCTTGATGGTGAAGTAAATTCCATGTCAACAAGCTACAAATCTTTGCCATTAAGTTCTCCAACCATGAAACTGAATCTCACTAGTCCTAAAAGGGGTCAAAAAAGAGAAGAAGGGTGGAAAGAAGTTGTTAGAAGACTGGTCTTACACCCTTGA
Peptide sequence:
MEAASGGYAEVGRVLLDKGADVNAPPVPSSRDTALTIAADKGHYKFCELLINRGAHIDVRNKKGNTPLWLASNGGHFDVVQLLVQAGADVDAADNRKITPLMSAFRKGHVKVVQYLVKEVSQFPSDIECMRYIATITDKELLKKCHQCVETIVKAKDQQAAEANKNASILLKELDLEKSREESRKQALAAKREKRKEKRKKKKEEQKRKQEDEENKPKENSEQPEGEDEENDEDVEQEIPIEPPSATTTTTIGISATSTTFTNVFGKKRANVVTTPSTNRKNKKNKTKESPPTAHLILPEPHISLAQQKADKNKINGEPRGGGAGGNSDSDNIDSTDCNSESSSGGKSQEFSFPVDVNPASDKRCSTVVSSQEEKAVTTTSKTQTRLDGEVNSMSTSYKSLPLSSPTMKLNLTSPKRGQKREEGWKEVVRRLVLHP
IRESfinder result
IRESfinder score
0.490035748
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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