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mmu_circAnkfy1_889
Basic information of circular RNA
PanCircBase ID:
mmu_circAnkfy1_889
mm39_Chr. Position ID:
chr11|72646515|72652335|+
Gene name:
Ankfy1
Transcript:
ENSMUST00000127610.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
6
Exon/intron indexes:
18,19,20,21,22,23
Exon/intron offsets:
0,1603,2763,4226,4550,5673
Genomic length:
5820
Exon/intron sizes in nt:
20,11,77,14,89,11,25,100
Flanking exon/introns:
chr11:72645319-72646515|chr11:72652335-72655602
Splice length:
889
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GATGCAGAAGGGAGAACGCCTGTCCATGTGGCCATCAGCAACCAGCATAGCGTCATCATTCAGCTGTTGATCTCTCACCCCAACATTGAGTTGAGCGTCCGGGACAGACAAGGGCTGACTCCTTTTGCCTGTGCCATGACTTACAAGAACAATAAGGCAGCTGAAGCCATTTTAAAACGAGAGTCTGGGGCTGCTGAGCAGGTAGATAATAAAGGTCGGAATTTTCTCCATGTGGCAGTTCAGAACTCTGACATTGAAAGTGTCTTGTTCCTGATCAGTGTCCAGGCCAATGTGAACTCCAGAGTCCAGGATGCTTCCAAGTTGACCCCTCTGCACCTTGCTGTTCAGGCCGGCTCAGAGATCATTGTCCGAAATCTGCTTCTTGCAGGAGCCAAGGTGAATGAGTTAACCAAGCATCGTCAGACCGCTCTCCATCTCGCTGCACAGCAGGACCTGCCCACCATCTGCTCAGTCCTCCTAGAGAATGGAGTGGACTTCGCTGCTGTGGATGAGAATGGAAATAATGCTCTTCATCTTGCGGTCATGCATGGTCGACTCAACAATATCCGGGCTCTTCTGACAGAGTGTACAGTAGATGCTGAAGCCTTTAATCTGAGAGGCCAGTCACCACTACACATTTTGGGACAGTATGGCAAAGAAAATGCAGCAGCCATCTTTGATCTCTTCTTAGAGTGTATGCCTGAGTATCCTCTGGACAAACCAGATGCAGAAGGCAACACAGTGCTGCTCTTAGCATACATGAAAGGGAATGCCAACTTGTGCCGTGCCATCGTTCGGTCTGGGGTTCGCCTTGGGGTGAATAATAACCAAGGAGTCAACATTTTCAACTACCAGGTTGCCACTAAGCAGCTTCTGTTTAGATTATTAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ATCGTTCGGTCTGGGGTTC
59.4
ACGCTATGCTGGTTGCTGAT
60.11
153
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ATCGTTCGGTCTGGGGTTC
59.4
ATGACGCTATGCTGGTTGCT
60.11
156
PCR template sequence:
ATCGTTCGGTCTGGGGTTCGCCTTGGGGTGAATAATAACCAAGGAGTCAACATTTTCAACTACCAGGTTGCCACTAAGCAGCTTCTGTTTAGATTATTAGGATGCAGAAGGGAGAACGCCTGTCCATGTGGCCATCAGCAACCAGCATAGCGTCATCATTCAGCTGTTGATCTCTCACCCCAACATTGAGTTGAGCGTCC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
13
Interacting miRNAs (#binding sites)
mmu-miR-183-5p(2), mmu-miR-23b-3p(1), mmu-miR-134-5p(1), mmu-miR-15a-5p(1), mmu-miR-135a-5p(1), mmu-miR-204-5p(1), mmu-miR-322-5p(1), mmu-miR-15b-5p(1), mmu-miR-23a-3p(1), mmu-miR-136-5p(1), mmu-miR-22-3p(1), mmu-miR-3470b(1), mmu-miR-3105-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TTTAGATTATTAGGATGCAGAAGGGA
siRNA sets
TTCTGCATCCTAATAATCT, CTTCTGCATCCTAATAATC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCANKFY1_889_ORF_1
1.2204
0.219553935
0.975504079
804
ORF sequence:
ATGACTTACAAGAACAATAAGGCAGCTGAAGCCATTTTAAAACGAGAGTCTGGGGCTGCTGAGCAGGTAGATAATAAAGGTCGGAATTTTCTCCATGTGGCAGTTCAGAACTCTGACATTGAAAGTGTCTTGTTCCTGATCAGTGTCCAGGCCAATGTGAACTCCAGAGTCCAGGATGCTTCCAAGTTGACCCCTCTGCACCTTGCTGTTCAGGCCGGCTCAGAGATCATTGTCCGAAATCTGCTTCTTGCAGGAGCCAAGGTGAATGAGTTAACCAAGCATCGTCAGACCGCTCTCCATCTCGCTGCACAGCAGGACCTGCCCACCATCTGCTCAGTCCTCCTAGAGAATGGAGTGGACTTCGCTGCTGTGGATGAGAATGGAAATAATGCTCTTCATCTTGCGGTCATGCATGGTCGACTCAACAATATCCGGGCTCTTCTGACAGAGTGTACAGTAGATGCTGAAGCCTTTAATCTGAGAGGCCAGTCACCACTACACATTTTGGGACAGTATGGCAAAGAAAATGCAGCAGCCATCTTTGATCTCTTCTTAGAGTGTATGCCTGAGTATCCTCTGGACAAACCAGATGCAGAAGGCAACACAGTGCTGCTCTTAGCATACATGAAAGGGAATGCCAACTTGTGCCGTGCCATCGTTCGGTCTGGGGTTCGCCTTGGGGTGAATAATAACCAAGGAGTCAACATTTTCAACTACCAGGTTGCCACTAAGCAGCTTCTGTTTAGATTATTAGGATGCAGAAGGGAGAACGCCTGTCCATGTGGCCATCAGCAACCAGCATAG
Peptide sequence:
MTYKNNKAAEAILKRESGAAEQVDNKGRNFLHVAVQNSDIESVLFLISVQANVNSRVQDASKLTPLHLAVQAGSEIIVRNLLLAGAKVNELTKHRQTALHLAAQQDLPTICSVLLENGVDFAAVDENGNNALHLAVMHGRLNNIRALLTECTVDAEAFNLRGQSPLHILGQYGKENAAAIFDLFLECMPEYPLDKPDAEGNTVLLLAYMKGNANLCRAIVRSGVRLGVNNNQGVNIFNYQVATKQLLFRLLGCRRENACPCGHQQPA
IRESfinder result
IRESfinder score
0.534858864
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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