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mmu_circAnkfy1_689
Basic information of circular RNA
PanCircBase ID:
mmu_circAnkfy1_689
mm39_Chr. Position ID:
chr11|72623073|72631427|+
Gene name:
Ankfy1
Transcript:
ENSMUST00000127610.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
7,8,9,10
Exon/intron offsets:
0,6004,7741,8154
Genomic length:
8354
Exon/intron sizes in nt:
1,66,20,51,18,200
Flanking exon/introns:
chr11:72621447-72623073|chr11:72631427-72637681
Splice length:
689
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
CTACCTGGGAAGCTTAACGAAACAGATCATAATGGAGATCTTGCGTTAGATCTGGCTCTTTCTCGGCGACTAGAGAGTATTGCCACCACTCTGGTTAGTCATAAAGCTGATGTGGATATGGTAGACAAAAATGGCTGGAGCTTATTACATAAAGGGATCCAAAGAGGAGATCTTTTTGCTTCTACATTCCTCATTAAGAACGGGGCCCTTGTCAATGCTGCCACAGCAGGAGCCCAGGAGACCCCATTACACCTTGTAGCATTGTACAGTCCAAAGAAATACTCAGCAGATGTGATGTCTGAGATGGCACAGATTGCGGAGGCCCTCCTGCAGGCTGGTGCCAACCCCAACATGCAGGATAGCAAGGGCAGGACTCCTTTACACTTGTCCATCATGGCCAGGAACGACTGTGTGTTCAGTCAGCTGCTGCAGTGTAAACAGTACGTAGGGCGGCCTGGAGAAATGCAAAGCGGTTGTGAGGGTGGGATTATTAGATTTAGAGCTGAAAGATCACGAAGGCAGCACTGCACTCTGGCTGGCCGTCCAGTACATCACTGTGTCTTCAGACCAGTCTGTAAACCCCTTTGAAGACCTCCCTGTGGTGAATGGGACATCATTTGATGAGAATAGCTTTGCAGCCAGACTCATTCAACGCGGCAGCAACACTGATGCTCCTGATGTGATGACAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CTCCCTGTGGTGAATGGGAC
60.04
CTAGTCGCCGAGAAAGAGCC
60.25
170
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GACCTCCCTGTGGTGAATGG
60.04
CTAGTCGCCGAGAAAGAGCC
60.25
173
PCR template sequence:
GACCTCCCTGTGGTGAATGGGACATCATTTGATGAGAATAGCTTTGCAGCCAGACTCATTCAACGCGGCAGCAACACTGATGCTCCTGATGTGATGACAGCTACCTGGGAAGCTTAACGAAACAGATCATAATGGAGATCTTGCGTTAGATCTGGCTCTTTCTCGGCGACTAGAGAGTATTGCCACCACTCTGGTTAGTC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
8
Interacting miRNAs (#binding sites)
mmu-miR-1a-3p(1), mmu-miR-206-3p(1), mmu-miR-103-3p(1), mmu-miR-204-5p(1), mmu-miR-136-5p(1), mmu-miR-96-5p(1), mmu-miR-370-3p(1), mmu-miR-107-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GATGTGATGACAGCTACCTGGGAAGC
siRNA sets
TTCCCAGGTAGCTGTCATC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.424070244
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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