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mmu_circAnk3_430
Basic information of circular RNA
PanCircBase ID:
mmu_circAnk3_430
mm39_Chr. Position ID:
chr10|69733870|69740340|+
Gene name:
Ank3
Transcript:
ENSMUST00000218680.2
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
20,21,22,23,24
Exon/intron offsets:
0,187,953,6157,6407
Genomic length:
6470
Exon/intron sizes in nt:
99,99,96,73,63
Flanking exon/introns:
chr10:69729373-69733870|chr10:69740340-69763524
Splice length:
430
Other database IDs:
circAtlas: mmu-Ank3_0060
Human Ortholog IDs:
Mature spliced Sequence:
ATGGGCTACACCCCGCTCCATGTGGGCTGTCACTATGGAAATATCAAAATAGTCAATTTTCTGCTGCAGCATTCTGCAAAAGTTAATGCCAAGACGAAGAATGGATACACAGCACTGCACCAGGCTGCTCAGCAGGGCCACACGCATATCATCAATGTCTTGCTTCAGAACAACGCCTCCCCCAATGAACTCACTGTGAATGGGAACACAGCTCTGGCCATCGCCCGGCGCCTTGGTTACATCTCGGTGGTTGACACACTGAAGGTCGTGACGGAGGAAATTATGACCACCACTACCATCACGGAGAAGCACAAAATGAATGTCCCAGAAACGATGAATGAAGTCCTCGATATGTCAGACGATGAAGTAAGGAAAGCCAGCGCCCCCGAAAAGCTCAGTGATGGGGAATATATCTCAGACGGTGAAGAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCCCCGAAAAGCTCAGTGAT
60.04
TCGTCTTGGCATTAACTTTTGCA
59.68
145
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CCCCCGAAAAGCTCAGTGAT
60.04
CGTCTTGGCATTAACTTTTGCA
58.36
144
PCR template sequence:
ACGATGAATGAAGTCCTCGATATGTCAGACGATGAAGTAAGGAAAGCCAGCGCCCCCGAAAAGCTCAGTGATGGGGAATATATCTCAGACGGTGAAGAAGATGGGCTACACCCCGCTCCATGTGGGCTGTCACTATGGAAATATCAAAATAGTCAATTTTCTGCTGCAGCATTCTGCAAAAGTTAATGCCAAGACGAAGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-27b-3p(1), mmu-miR-27a-3p(1), mmu-miR-181b-5p(1), mmu-miR-370-3p(1), mmu-miR-181d-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GACGGTGAAGAAGATGGGCTACACCC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCANK3_430_ORF_1
1.2164
0.283780067
0.867925932
516
ORF sequence:
ATGGGCTACACCCCGCTCCATGTGGGCTGTCACTATGGAAATATCAAAATAGTCAATTTTCTGCTGCAGCATTCTGCAAAAGTTAATGCCAAGACGAAGAATGGATACACAGCACTGCACCAGGCTGCTCAGCAGGGCCACACGCATATCATCAATGTCTTGCTTCAGAACAACGCCTCCCCCAATGAACTCACTGTGAATGGGAACACAGCTCTGGCCATCGCCCGGCGCCTTGGTTACATCTCGGTGGTTGACACACTGAAGGTCGTGACGGAGGAAATTATGACCACCACTACCATCACGGAGAAGCACAAAATGAATGTCCCAGAAACGATGAATGAAGTCCTCGATATGTCAGACGATGAAGTAAGGAAAGCCAGCGCCCCCGAAAAGCTCAGTGATGGGGAATATATCTCAGACGGTGAAGAAGATGGGCTACACCCCGCTCCATGTGGGCTGTCACTATGGAAATATCAAAATAGTCAATTTTCTGCTGCAGCATTCTGCAAAAGTTAA
Peptide sequence:
MGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLLQNNASPNELTVNGNTALAIARRLGYISVVDTLKVVTEEIMTTTTITEKHKMNVPETMNEVLDMSDDEVRKASAPEKLSDGEYISDGEEDGLHPAPCGLSLWKYQNSQFSAAAFCKS
IRESfinder result
IRESfinder score
0.159085841
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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