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mmu_circAnk2_99_1
Basic information of circular RNA
PanCircBase ID:
mmu_circAnk2_99_1
mm39_Chr. Position ID:
chr3|126850641|126850740|-
Gene name:
Ank2
Transcript:
ENSMUST00000182078.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
1
Exon/intron indexes:
6
Exon/intron offsets:
0
Genomic length:
99
Exon/intron sizes in nt:
99
Flanking exon/introns:
chr3:126846522-126850641|chr3:126850740-126871150
Splice length:
99
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
AATGGCTTCACTCCTTTGTACATGGCTGCCCAGGAGAATCACATTGATGTTGTAAAATATTTGCTAGAAAATGGAGCTAACCAGAGTACTGCTACAGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGGAGCTAACCAGAGTACTGC
59.17
ATGTGATTCTCCTGGGCAGC
60.11
72
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGAGCTAACCAGAGTACTGCT
58.9
ATGTGATTCTCCTGGGCAGC
60.11
71
PCR template sequence:
TGTAAAATATTTGCTAGAAAATGGAGCTAACCAGAGTACTGCTACAGAGAATGGCTTCACTCCTTTGTACATGGCTGCCCAGGAGAATCACATTGATG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-136-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TACTGCTACAGAGAATGGCTTCACTC
siRNA sets
GAAGCCATTCTCTGTAGCA, TGAAGCCATTCTCTGTAGC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCANK2_99_1_ORF_1
1.2231
0.166887893
0.266279869
177
ORF sequence:
ATGGCTGCCCAGGAGAATCACATTGATGTTGTAAAATATTTGCTAGAAAATGGAGCTAACCAGAGTACTGCTACAGAGAATGGCTTCACTCCTTTGTACATGGCTGCCCAGGAGAATCACATTGATGTTGTAAAATATTTGCTAGAAAATGGAGCTAACCAGAGTACTGCTACAGAG
Peptide sequence:
MAAQENHIDVVKYLLENGANQSTATENGFTPLYMAAQENHIDVVKYLLENGANQSTATE
IRESfinder result
IRESfinder score
0.381245009
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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