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mmu_circAnapc7_527
Basic information of circular RNA
PanCircBase ID:
mmu_circAnapc7_527
mm39_Chr. Position ID:
chr5|122570803|122576309|+
Gene name:
Anapc7
Transcript:
ENSMUST00000122010.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
5,6,7,8
Exon/intron offsets:
0,643,2778,5388
Genomic length:
5506
Exon/intron sizes in nt:
1,12,15,41,43,118
Flanking exon/introns:
chr5:122567668-122570803|chr5:122576309-122577381
Splice length:
527
Other database IDs:
circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_6627
Human Ortholog IDs:
Mature spliced Sequence:
ATAAACATGATGCTGGCAAACCTGTACAAGAAGGCTGGTCAGGAGCGCCCATCAGTCACAAGCTATAAGGAGGTCCTGCGGCAGTGCCCTTTGGCCCTTGACGCCATTCTAGGTTTGTTATCCCTGTCTGTGAAAGGCGCAGAGGTGGCATCAATGACGATGAATGTGATCCAGACTGTGCCCAACTTGGACTGGCTGTCCGTGTGGATTAAAGCGTATGCTTTTGTGCACACCGGTGACAACTCGAGAGCAATCAACACCATCTGTTCACTAGAGAAAAAATCATTACTGCGAGACAACGTGGACCTGCTGGGGAGCCTGGCCGACCTGTACTTCAGAGCAGGGGACAGCAAGAACTCTGTCCTCAAGTTTGAGCAGGCCCAGATGCTGGATCCCTACTTGATAAGAGGGATGGATGTTTACGGCTACCTCCTGGCACGAGAAGGGCGGCTGGAGGATGTGGAGAACCTTGGCTGCCGCCTCTTCAACATTTCTGATCAGCATGCAGAGCCCTGGGTGGTCTCCGG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCTGGAGGATGTGGAGAACC
60.11
TAGCTTGTGACTGATGGGCG
60.11
143
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGCTGGAGGATGTGGAGAAC
60.11
TAGCTTGTGACTGATGGGCG
60.11
144
PCR template sequence:
ACCTCCTGGCACGAGAAGGGCGGCTGGAGGATGTGGAGAACCTTGGCTGCCGCCTCTTCAACATTTCTGATCAGCATGCAGAGCCCTGGGTGGTCTCCGGATAAACATGATGCTGGCAAACCTGTACAAGAAGGCTGGTCAGGAGCGCCCATCAGTCACAAGCTATAAGGAGGTCCTGCGGCAGTGCCCTTTGGCCCTTG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
14
Interacting miRNAs (#binding sites)
mmu-miR-134-5p(1), mmu-miR-181a-5p(1), mmu-miR-30e-5p(1), mmu-miR-26a-5p(1), mmu-miR-30a-5p(1), mmu-miR-30c-5p(1), mmu-miR-181b-5p(1), mmu-miR-30d-5p(1), mmu-let-7d-5p(1), mmu-miR-30b-5p(1), mmu-miR-26b-5p(1), mmu-miR-324-3p(1), mmu-miR-338-3p(1), mmu-miR-181d-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GGGTGGTCTCCGGATAAACATGATGC
siRNA sets
ATCATGTTTATCCGGAGAC, TGTTTATCCGGAGACCACC, CATGTTTATCCGGAGACCA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCANAPC7_527_ORF_1
1.2354
0.35725366
0.907113575
525
ORF sequence:
ATGATGCTGGCAAACCTGTACAAGAAGGCTGGTCAGGAGCGCCCATCAGTCACAAGCTATAAGGAGGTCCTGCGGCAGTGCCCTTTGGCCCTTGACGCCATTCTAGGTTTGTTATCCCTGTCTGTGAAAGGCGCAGAGGTGGCATCAATGACGATGAATGTGATCCAGACTGTGCCCAACTTGGACTGGCTGTCCGTGTGGATTAAAGCGTATGCTTTTGTGCACACCGGTGACAACTCGAGAGCAATCAACACCATCTGTTCACTAGAGAAAAAATCATTACTGCGAGACAACGTGGACCTGCTGGGGAGCCTGGCCGACCTGTACTTCAGAGCAGGGGACAGCAAGAACTCTGTCCTCAAGTTTGAGCAGGCCCAGATGCTGGATCCCTACTTGATAAGAGGGATGGATGTTTACGGCTACCTCCTGGCACGAGAAGGGCGGCTGGAGGATGTGGAGAACCTTGGCTGCCGCCTCTTCAACATTTCTGATCAGCATGCAGAGCCCTGGGTGGTCTCCGGATAA
Peptide sequence:
MMLANLYKKAGQERPSVTSYKEVLRQCPLALDAILGLLSLSVKGAEVASMTMNVIQTVPNLDWLSVWIKAYAFVHTGDNSRAINTICSLEKKSLLRDNVDLLGSLADLYFRAGDSKNSVLKFEQAQMLDPYLIRGMDVYGYLLAREGRLEDVENLGCRLFNISDQHAEPWVVSG
IRESfinder result
IRESfinder score
0.417850507
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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