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mmu_circAnapc4_916
Basic information of circular RNA
PanCircBase ID:
mmu_circAnapc4_916
mm39_Chr. Position ID:
chr5|53000162|53014569|+
Gene name:
Anapc4
Transcript:
ENSMUST00000031072.14
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
13
Exon/intron indexes:
7,8,9,10,11,12,13,14,15,16,17,18,19
Exon/intron offsets:
0,666,2503,3811,5170,5773,5934,7637,7822,12763,13023,13987,14350
Genomic length:
14407
Exon/intron sizes in nt:
85,105,84,87,65,43,77,101,52,56,47,57,57
Flanking exon/introns:
chr5:52999410-53000162|chr5:53014569-53016454
Splice length:
916
Other database IDs:
circAtlas: mmu-Anapc4_0014
circAtlas: hsa-SCAF8_0001
Human Ortholog IDs:
Mature spliced Sequence:
GCTGAACATCCTTGTCCTTGGAGGAAGCTCTGGATTTATTGAGCTTTATGCTTATGGGATGTTCAAAATTGCCCGAGTAACAGGGATTGCCGGAACTTGTATTGCTCTGTGTTTATCAAGTGATTTGAAGTCATTATCAGTGGTCACAGAGGTTTCCAGCGGTGGCGAATCAGAAGTTTCATATTTTCAGCTTGAAACCAATCTGCTGTATTCCTTCTTACCTGAAGTAACTCGTATGGCCAGGAAGTTTACTCACATTTCAGCTCTCTTACAGTATATAAATTTGTCACTGACATGTATGTGTGAAGCATGGGAAGAAATTCTGATGCAGATGGACTCCCGTCTTACCAAGTTTGTACAGGAAAAACCTACAACCACGTCAGTGCAGGATGAGTTCATGCACTTGCTGCTGTGGGGGAAAGCCAGTGCTGAACTTCAGACTCTCCTGATGAACCAGTTAACAGTGAAGGGCTTAAAAAAGCTTGGCCAGTCTATAGAGTCATCATATTCCAGTATACAAAAACTGGTTATAAGTCACTTACAGAGTGGCTCTGAGTCTTTACTGTATCATTTGAGTGAACTTAAAGGAATGGCTTCATGGAAACAAAAATACGAACCTCTAGGACTGGATGCTGCAGGCATTGAAGATGCTATTACTGCTGTGGGTTCTTTCATCCTCAAAGCAAACGAACTTCTCCAAGTTATAGACAGTAGTATGAAAAACTTCAAGGCATTTTTTCGGTGGCTGTATGTAGCAATGCTGAGAATGACAGAGGACCATGTGCTTCCTGAGCTGAACAAGATGACTCAGAAGGATATCACATTTGTTGCTGAATTCCTCACTGAACATTTCAATGAGGCTCCAGACCTTTATAATAGGAAAGGAAAATACTTCAATGTTGAAAGAGTTGGTCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCAATGAGGCTCCAGACCTT
58.34
AAGTTCCGGCAATCCCTGTT
59.89
163
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TTCAATGAGGCTCCAGACCT
58.34
AAGTTCCGGCAATCCCTGTT
59.89
164
PCR template sequence:
TATCACATTTGTTGCTGAATTCCTCACTGAACATTTCAATGAGGCTCCAGACCTTTATAATAGGAAAGGAAAATACTTCAATGTTGAAAGAGTTGGTCAGGCTGAACATCCTTGTCCTTGGAGGAAGCTCTGGATTTATTGAGCTTTATGCTTATGGGATGTTCAAAATTGCCCGAGTAACAGGGATTGCCGGAACTTGT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
14
Interacting miRNAs (#binding sites)
mmu-miR-16-5p(1), mmu-miR-221-3p(1), mmu-miR-322-5p(1), mmu-miR-15b-5p(1), mmu-miR-222-3p(1), mmu-miR-203-3p(1), mmu-miR-381-3p(1), mmu-miR-483-5p(1), mmu-miR-340-5p(1), mmu-miR-5101(1), mmu-miR-335-3p(1), mmu-miR-532-5p(1), mmu-miR-3470b(1), mmu-miR-466b-3p(1)
siRNAs for circular RNA silencing
Junction sequence
AAGAGTTGGTCAGGCTGAACATCCTT
siRNA sets
ATGTTCAGCCTGACCAACT, AAGGATGTTCAGCCTGACC, TTCAGCCTGACCAACTCTT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCANAPC4_916_ORF_1
0.8321
0.027870373
0.968719888
978
ORF sequence:
ATGAGGCTCCAGACCTTTATAATAGGAAAGGAAAATACTTCAATGTTGAAAGAGTTGGTCAGGCTGAACATCCTTGTCCTTGGAGGAAGCTCTGGATTTATTGAGCTTTATGCTTATGGGATGTTCAAAATTGCCCGAGTAACAGGGATTGCCGGAACTTGTATTGCTCTGTGTTTATCAAGTGATTTGAAGTCATTATCAGTGGTCACAGAGGTTTCCAGCGGTGGCGAATCAGAAGTTTCATATTTTCAGCTTGAAACCAATCTGCTGTATTCCTTCTTACCTGAAGTAACTCGTATGGCCAGGAAGTTTACTCACATTTCAGCTCTCTTACAGTATATAAATTTGTCACTGACATGTATGTGTGAAGCATGGGAAGAAATTCTGATGCAGATGGACTCCCGTCTTACCAAGTTTGTACAGGAAAAACCTACAACCACGTCAGTGCAGGATGAGTTCATGCACTTGCTGCTGTGGGGGAAAGCCAGTGCTGAACTTCAGACTCTCCTGATGAACCAGTTAACAGTGAAGGGCTTAAAAAAGCTTGGCCAGTCTATAGAGTCATCATATTCCAGTATACAAAAACTGGTTATAAGTCACTTACAGAGTGGCTCTGAGTCTTTACTGTATCATTTGAGTGAACTTAAAGGAATGGCTTCATGGAAACAAAAATACGAACCTCTAGGACTGGATGCTGCAGGCATTGAAGATGCTATTACTGCTGTGGGTTCTTTCATCCTCAAAGCAAACGAACTTCTCCAAGTTATAGACAGTAGTATGAAAAACTTCAAGGCATTTTTTCGGTGGCTGTATGTAGCAATGCTGAGAATGACAGAGGACCATGTGCTTCCTGAGCTGAACAAGATGACTCAGAAGGATATCACATTTGTTGCTGAATTCCTCACTGAACATTTCAATGAGGCTCCAGACCTTTATAATAGGAAAGGAAAATACTTCAATGTTGAAAGAGTTGGTCAG
Peptide sequence:
MRLQTFIIGKENTSMLKELVRLNILVLGGSSGFIELYAYGMFKIARVTGIAGTCIALCLSSDLKSLSVVTEVSSGGESEVSYFQLETNLLYSFLPEVTRMARKFTHISALLQYINLSLTCMCEAWEEILMQMDSRLTKFVQEKPTTTSVQDEFMHLLLWGKASAELQTLLMNQLTVKGLKKLGQSIESSYSSIQKLVISHLQSGSESLLYHLSELKGMASWKQKYEPLGLDAAGIEDAITAVGSFILKANELLQVIDSSMKNFKAFFRWLYVAMLRMTEDHVLPELNKMTQKDITFVAEFLTEHFNEAPDLYNRKGKYFNVERVGQ
IRESfinder result
IRESfinder score
0.720114194
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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