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mmu_circAnapc4_562
Basic information of circular RNA
PanCircBase ID:
mmu_circAnapc4_562
mm39_Chr. Position ID:
chr5|53007799|53018682|+
Gene name:
Anapc4
Transcript:
ENSMUST00000031072.14
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
8
Exon/intron indexes:
14,15,16,17,18,19,20,21
Exon/intron offsets:
0,185,5126,5386,6350,6713,8655,10785
Genomic length:
10883
Exon/intron sizes in nt:
101,52,56,47,57,57,94,98
Flanking exon/introns:
chr5:53006173-53007799|chr5:53018682-53019290
Splice length:
562
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
TGGCTCTGAGTCTTTACTGTATCATTTGAGTGAACTTAAAGGAATGGCTTCATGGAAACAAAAATACGAACCTCTAGGACTGGATGCTGCAGGCATTGAAGATGCTATTACTGCTGTGGGTTCTTTCATCCTCAAAGCAAACGAACTTCTCCAAGTTATAGACAGTAGTATGAAAAACTTCAAGGCATTTTTTCGGTGGCTGTATGTAGCAATGCTGAGAATGACAGAGGACCATGTGCTTCCTGAGCTGAACAAGATGACTCAGAAGGATATCACATTTGTTGCTGAATTCCTCACTGAACATTTCAATGAGGCTCCAGACCTTTATAATAGGAAAGGAAAATACTTCAATGTTGAAAGAGTTGGTCAGTATTTGAAAGATGAAGACGACGACCTTGTGTCACCCCCTAACACAGAAGGAAACCAGTGGTATGACTTCCTCCAAAACAGCACCCACCTGAAAGAAAGTCCTTTGCTGTTTCCTTATTACCCTCGAAAATCATTGCATTTTGTGAAAAGGCGGATGGAGAACGTTATTGATCAGTGTTTGCAAAAGCCAGCA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AAAGGCGGATGGAGAACGTT
59.96
CCTGCAGCATCCAGTCCTAG
59.89
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AAAAGGCGGATGGAGAACGT
59.96
CCTGCAGCATCCAGTCCTAG
59.89
141
PCR template sequence:
AGAAAGTCCTTTGCTGTTTCCTTATTACCCTCGAAAATCATTGCATTTTGTGAAAAGGCGGATGGAGAACGTTATTGATCAGTGTTTGCAAAAGCCAGCATGGCTCTGAGTCTTTACTGTATCATTTGAGTGAACTTAAAGGAATGGCTTCATGGAAACAAAAATACGAACCTCTAGGACTGGATGCTGCAGGCATTGAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
12
Interacting miRNAs (#binding sites)
mmu-miR-200a-3p(1), mmu-miR-141-3p(1), mmu-miR-221-3p(1), mmu-miR-222-3p(1), mmu-miR-203-3p(1), mmu-miR-29b-1-5p(1), mmu-miR-340-5p(1), mmu-miR-29b-2-5p(1), mmu-miR-5101(1), mmu-miR-335-3p(1), mmu-miR-532-5p(1), mmu-miR-466b-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GCAAAAGCCAGCATGGCTCTGAGTCT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCANAPC4_562_ORF_1
0.9848
0.115410787
0.699323289
528
ORF sequence:
ATGGCTTCATGGAAACAAAAATACGAACCTCTAGGACTGGATGCTGCAGGCATTGAAGATGCTATTACTGCTGTGGGTTCTTTCATCCTCAAAGCAAACGAACTTCTCCAAGTTATAGACAGTAGTATGAAAAACTTCAAGGCATTTTTTCGGTGGCTGTATGTAGCAATGCTGAGAATGACAGAGGACCATGTGCTTCCTGAGCTGAACAAGATGACTCAGAAGGATATCACATTTGTTGCTGAATTCCTCACTGAACATTTCAATGAGGCTCCAGACCTTTATAATAGGAAAGGAAAATACTTCAATGTTGAAAGAGTTGGTCAGTATTTGAAAGATGAAGACGACGACCTTGTGTCACCCCCTAACACAGAAGGAAACCAGTGGTATGACTTCCTCCAAAACAGCACCCACCTGAAAGAAAGTCCTTTGCTGTTTCCTTATTACCCTCGAAAATCATTGCATTTTGTGAAAAGGCGGATGGAGAACGTTATTGATCAGTGTTTGCAAAAGCCAGCATGGCTCTGA
Peptide sequence:
MASWKQKYEPLGLDAAGIEDAITAVGSFILKANELLQVIDSSMKNFKAFFRWLYVAMLRMTEDHVLPELNKMTQKDITFVAEFLTEHFNEAPDLYNRKGKYFNVERVGQYLKDEDDDLVSPPNTEGNQWYDFLQNSTHLKESPLLFPYYPRKSLHFVKRRMENVIDQCLQKPAWL
IRESfinder result
IRESfinder score
0.585012406
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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