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mmu_circAnapc4_346
Basic information of circular RNA
PanCircBase ID:
mmu_circAnapc4_346
mm39_Chr. Position ID:
chr5|52999246|53002749|+
Gene name:
Anapc4
Transcript:
ENSMUST00000031072.14
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
5,6,7,8,9
Exon/intron offsets:
0,119,916,1582,3419
Genomic length:
3503
Exon/intron sizes in nt:
27,45,85,105,84
Flanking exon/introns:
chr5:52999132-52999246|chr5:53002749-53003973
Splice length:
346
Other database IDs:
circAtlas: hsa-SCAF8_0001
Human Ortholog IDs:
Mature spliced Sequence:
TTACAACAGCACCTCAAAAATATTTAGTGAAGAAAATTCTGATGAAATTATCAAGCTCTTGGGAGATGTCAGGCTGAACATCCTTGTCCTTGGAGGAAGCTCTGGATTTATTGAGCTTTATGCTTATGGGATGTTCAAAATTGCCCGAGTAACAGGGATTGCCGGAACTTGTATTGCTCTGTGTTTATCAAGTGATTTGAAGTCATTATCAGTGGTCACAGAGGTTTCCAGCGGTGGCGAATCAGAAGTTTCATATTTTCAGCTTGAAACCAATCTGCTGTATTCCTTCTTACCTGAAGTAACTCGTATGGCCAGGAAGTTTACTCACATTTCAGCTCTCTTACAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACTCGTATGGCCAGGAAGTT
58.72
GCTTCCTCCAAGGACAAGGA
59.31
145
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AACTCGTATGGCCAGGAAGT
58.72
GCTTCCTCCAAGGACAAGGA
59.31
146
PCR template sequence:
AGTTTCATATTTTCAGCTTGAAACCAATCTGCTGTATTCCTTCTTACCTGAAGTAACTCGTATGGCCAGGAAGTTTACTCACATTTCAGCTCTCTTACAGTTACAACAGCACCTCAAAAATATTTAGTGAAGAAAATTCTGATGAAATTATCAAGCTCTTGGGAGATGTCAGGCTGAACATCCTTGTCCTTGGAGGAAGC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-381-3p(1)
siRNAs for circular RNA silencing
Junction sequence
AGCTCTCTTACAGTTACAACAGCACC
siRNA sets
GTTGTAACTGTAAGAGAGC, TTGTAACTGTAAGAGAGCT, TGTTGTAACTGTAAGAGAG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCANAPC4_346_ORF_1
0.6204
-0.093057401
0.061082956
243
ORF sequence:
ATGTTCAAAATTGCCCGAGTAACAGGGATTGCCGGAACTTGTATTGCTCTGTGTTTATCAAGTGATTTGAAGTCATTATCAGTGGTCACAGAGGTTTCCAGCGGTGGCGAATCAGAAGTTTCATATTTTCAGCTTGAAACCAATCTGCTGTATTCCTTCTTACCTGAAGTAACTCGTATGGCCAGGAAGTTTACTCACATTTCAGCTCTCTTACAGTTACAACAGCACCTCAAAAATATTTAG
Peptide sequence:
MFKIARVTGIAGTCIALCLSSDLKSLSVVTEVSSGGESEVSYFQLETNLLYSFLPEVTRMARKFTHISALLQLQQHLKNI
IRESfinder result
IRESfinder score
0.835256007
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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