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mmu_circAnapc2_842
Basic information of circular RNA
PanCircBase ID:
mmu_circAnapc2_842
mm39_Chr. Position ID:
chr2|25166327|25170498|+
Gene name:
Anapc2
Transcript:
ENSMUST00000028341.11
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
6
Exon/intron indexes:
5,6,7,8,9,10
Exon/intron offsets:
0,1320,2008,2856,3811,3967
Genomic length:
4171
Exon/intron sizes in nt:
12,01,18,18,21,42,76,200
Flanking exon/introns:
chr2:25164852-25166327|chr2:25170498-25174573
Splice length:
842
Other database IDs:
circAtlas: mmu-Anapc2_0001
circAtlas: hsa-SCAF8_0001
Human Ortholog IDs:
Mature spliced Sequence:
ACTTTCCAGACTCCCGGCCAGCCATTGAAGACCTTAAGTACTGCTTGGAGAGGACAGATCAGAGGCAGCAACTTCTTGTGTCCCTCAAGGTGGCCCTAGAGACTCGACTCCTCCACCCAGGTGTCAACACATGTGACATCATCACTCTCTACATTTCTGCCATCAAGGCATTACGTGTGCTAGATCCCTCTATGGTCATCCTGGAAGTGGCTTGTGAGCCCATCCGTCGATACCTGAGGACACGAGAGGACACCGTACGGCAGATTGTGGCTGGGCTAACCGGGGACTCAGATGGGACTGGGGACTTGGCTGTTGAGCTGTCTAAGACTGACCCTGCCTGCCTGGAGACCGGTCAGGACAGTGAGGATGATTCTGGAGAGCCCGAGGACTGGGTCCCTGACCCTGTGGATGCTGATCCAGTGAAGTCGAGTTCCAAGCGACGTTCTTCAGACATCATCAGCCTGCTGGTCAGCATCTATGGCAGCAAAGACCTCTTTATCAATGAGTACCGCTCGCTACTTGCAGATCGCCTTCTCCACCAATTCAGCTTCAGCCCTGAGAGGGAGATTCGTAATGTGGAGCTGTTGAAGCTGCGCTTTGGTGAAGCCCCCATGCACTTCTGTGAGGTCATGCTCAAGGACATGGCAGACTCCCGCCGCATCAATGCCAATATCCGAGAGGAGGATGAGAAGCGGCCTGTGGAAGAACAGCCACCATTTGGGGTCTATGCTGTCATATTGTCCAGTGAATTTTGGCCTCCCTTCAAAGATGAGAAGCTGGAGGTCCCTGAGGACATCAGGGCAGCCCTAGACGTTTACTGCAAGAAGTATGAGAAACTGAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ATGAGAAGCTGGAGGTCCCT
59.96
GCTGCCTCTGATCTGTCCTC
59.9
143
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ATGAGAAGCTGGAGGTCCCT
59.96
TTGCTGCCTCTGATCTGTCC
59.75
145
PCR template sequence:
CAGTGAATTTTGGCCTCCCTTCAAAGATGAGAAGCTGGAGGTCCCTGAGGACATCAGGGCAGCCCTAGACGTTTACTGCAAGAAGTATGAGAAACTGAAGACTTTCCAGACTCCCGGCCAGCCATTGAAGACCTTAAGTACTGCTTGGAGAGGACAGATCAGAGGCAGCAACTTCTTGTGTCCCTCAAGGTGGCCCTAGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-434-5p(1), mmu-miR-1198-5p(1), mmu-miR-532-5p(1), mmu-miR-1199-5p(1), mmu-miR-344-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TGAGAAACTGAAGACTTTCCAGACTC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCANAPC2_842_ORF_1
1.056
0.416483782
0.978040937
750
ORF sequence:
ATGGTCATCCTGGAAGTGGCTTGTGAGCCCATCCGTCGATACCTGAGGACACGAGAGGACACCGTACGGCAGATTGTGGCTGGGCTAACCGGGGACTCAGATGGGACTGGGGACTTGGCTGTTGAGCTGTCTAAGACTGACCCTGCCTGCCTGGAGACCGGTCAGGACAGTGAGGATGATTCTGGAGAGCCCGAGGACTGGGTCCCTGACCCTGTGGATGCTGATCCAGTGAAGTCGAGTTCCAAGCGACGTTCTTCAGACATCATCAGCCTGCTGGTCAGCATCTATGGCAGCAAAGACCTCTTTATCAATGAGTACCGCTCGCTACTTGCAGATCGCCTTCTCCACCAATTCAGCTTCAGCCCTGAGAGGGAGATTCGTAATGTGGAGCTGTTGAAGCTGCGCTTTGGTGAAGCCCCCATGCACTTCTGTGAGGTCATGCTCAAGGACATGGCAGACTCCCGCCGCATCAATGCCAATATCCGAGAGGAGGATGAGAAGCGGCCTGTGGAAGAACAGCCACCATTTGGGGTCTATGCTGTCATATTGTCCAGTGAATTTTGGCCTCCCTTCAAAGATGAGAAGCTGGAGGTCCCTGAGGACATCAGGGCAGCCCTAGACGTTTACTGCAAGAAGTATGAGAAACTGAAGACTTTCCAGACTCCCGGCCAGCCATTGAAGACCTTAAGTACTGCTTGGAGAGGACAGATCAGAGGCAGCAACTTCTTGTGTCCCTCAAGGTGGCCCTAG
Peptide sequence:
MVILEVACEPIRRYLRTREDTVRQIVAGLTGDSDGTGDLAVELSKTDPACLETGQDSEDDSGEPEDWVPDPVDADPVKSSSKRRSSDIISLLVSIYGSKDLFINEYRSLLADRLLHQFSFSPEREIRNVELLKLRFGEAPMHFCEVMLKDMADSRRINANIREEDEKRPVEEQPPFGVYAVILSSEFWPPFKDEKLEVPEDIRAALDVYCKKYEKLKTFQTPGQPLKTLSTAWRGQIRGSNFLCPSRWP
IRESfinder result
IRESfinder score
0.477393113
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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