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mmu_circAnapc1_529
Basic information of circular RNA
PanCircBase ID:
mmu_circAnapc1_529
mm39_Chr. Position ID:
chr2|128508484|128514077|-
Gene name:
Anapc1
Transcript:
ENSMUST00000014499.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
15,14,13,12,11
Exon/intron offsets:
0,2360,3085,3258,5497
Genomic length:
5593
Exon/intron sizes in nt:
141,135,66,91,96
Flanking exon/introns:
chr2:128507428-128508484|chr2:128514077-128516470
Splice length:
529
Other database IDs:
circAtlas: mmu-Anapc1_0068
Human Ortholog IDs:
Mature spliced Sequence:
AGAGAAAAATTCTCAAGCCTCCAAGGTATTTATAACGACTGACCTGTGTGGACAGAAGTTCTTGTGCTTTTTAGTGGAGGCCCAGCTTCAGTTACGCTGTGTAAAGTTTCAAGAGAGTAATGACAAGACTCAGCTTATCTTTGGCTCTGTCACCAACATACATGCAAAAGATGCAGCCCCAGTGGAGAAGATACACACCATGCTGGTCCTAGAAGGCAACGGGAACCTGGTGCTGTACACAGGAGTGGTGCGGGTGGGGAAAGTCTTTATCCCTGGACTTCCGGCTCCTTCCCTGACAATGTCCAACATGATGCCTCGGCCCAGTACCCCGCTCGACGGTGTTGGTACTCCAAAGCCTCTGAGTAAATTGCTTGGATCGATGGATGAGGTGGTTCTGTTGTCTCCAGTTCCAGAATTGAGGGATTCTTCAAAACTTAATGATTCTCTCTACAATGAGGATTGCACATTCCAGCAGCTTGGAACTTACATTCATTCTGTGCGAGACCCTGTTCATAACAGAGTCACTCTA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGATTGCACATTCCAGCAGC
60.18
GAAGCTGGGCCTCCACTAAA
59.67
162
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGATTGCACATTCCAGCAGC
60.18
GTAACTGAAGCTGGGCCTCC
60.39
168
PCR template sequence:
AAAACTTAATGATTCTCTCTACAATGAGGATTGCACATTCCAGCAGCTTGGAACTTACATTCATTCTGTGCGAGACCCTGTTCATAACAGAGTCACTCTAAGAGAAAAATTCTCAAGCCTCCAAGGTATTTATAACGACTGACCTGTGTGGACAGAAGTTCTTGTGCTTTTTAGTGGAGGCCCAGCTTCAGTTACGCTGT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-338-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CAGAGTCACTCTAAGAGAAAAATTCT
siRNA sets
TTTTCTCTTAGAGTGACTC, AATTTTTCTCTTAGAGTGA, ATTTTTCTCTTAGAGTGAC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCANAPC1_529_ORF_1
0.9565
0.140945602
0.443157021
372
ORF sequence:
ATGCTGGTCCTAGAAGGCAACGGGAACCTGGTGCTGTACACAGGAGTGGTGCGGGTGGGGAAAGTCTTTATCCCTGGACTTCCGGCTCCTTCCCTGACAATGTCCAACATGATGCCTCGGCCCAGTACCCCGCTCGACGGTGTTGGTACTCCAAAGCCTCTGAGTAAATTGCTTGGATCGATGGATGAGGTGGTTCTGTTGTCTCCAGTTCCAGAATTGAGGGATTCTTCAAAACTTAATGATTCTCTCTACAATGAGGATTGCACATTCCAGCAGCTTGGAACTTACATTCATTCTGTGCGAGACCCTGTTCATAACAGAGTCACTCTAAGAGAAAAATTCTCAAGCCTCCAAGGTATTTATAACGACTGA
Peptide sequence:
MLVLEGNGNLVLYTGVVRVGKVFIPGLPAPSLTMSNMMPRPSTPLDGVGTPKPLSKLLGSMDEVVLLSPVPELRDSSKLNDSLYNEDCTFQQLGTYIHSVRDPVHNRVTLREKFSSLQGIYND
IRESfinder result
IRESfinder score
0.658566927
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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