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mmu_circAnapc1_3349
Basic information of circular RNA
PanCircBase ID:
mmu_circAnapc1_3349
mm39_Chr. Position ID:
chr2|128487053|128527980|-
Gene name:
Anapc1
Transcript:
ENSMUST00000014499.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
26
Exon/intron indexes:
27,26,25,24,23,22,21,20,19,18,17,16,15,14,13,12,11,10,9,8,7,6,5,4,3,2
Exon/intron offsets:
0,3171,5062,7562,7718,8033,11992,13114,14603,15671,18741,20314,21431,23791,24516,24689,26928,29417,30537,30968,33077,34386,34975,35260,39368,40690
Genomic length:
40927
Exon/intron sizes in nt:
1,19,21,51,79,71,37,79,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,000
Flanking exon/introns:
chr2:128484637-128487053|chr2:128527980-128529072
Splice length:
3349
Other database IDs:
circAtlas: mmu-Anapc1_0032
Human Ortholog IDs:
Mature spliced Sequence:
AAATAATGTTCATATTTGGAACCCATGTCGAACTTCTCTGAAGAAAGGGCAACGATGATTGCAGCCGGGGATTTGCAGGAATTTGTTCCTTTTGGCCGAGACCACTGCAAGCACCACCCTAACGCTCTGAACCTTCAGCTTCGTCAGCTGCAGCCAGCCTCTGAGTTATGGTCTTCTGATGGTGCAGCTGGCTTGGTGGGATCTCTTCAGGAGGTTACAATCCATGAGAAACAGAAGGAAAGCTGGCAGTTAAGAAAAGGTGTGAGTGAGATTGGAGATGCAGCTGACTACGATGAAGAGCTCTATGTGGCTGGGAACATGGTTATCTGGAGCAAAGGGAGTAAAAGCCAGGCACTGGCAGTGTACAAAGCGTTCACAGTGGACAGCACTGTCCAGCAGGCATTGTGGTGTGACTTCATTATATCACAGGATAAGTCTGAAAAGATCTATAAGAGCCATGAACTAGAGAAATGCATATGTATATTACAGAGTTCATGTATGAACATGCATAGCATAGACGGGAAGGATTATATAGCTTCCTTACCTTTTCAGGTGGCAAATGTTTGGGCCACTAAATATGGCTTGCTGTTTGAACGATGCAGTTCTTCACATGAGGTACCTCCAAGTCTACCTAGAGAACCTTTGCCTACTATGTTCAGCATGCTGCACCCACTGGATGAGATAACTCCACTTGTTTGTAAATCCGGAAGTCTTTTTGGCTCATCACGGGTACAGTATGTTGTAGATCCAGCAGTGAAAATTGTGTTTCTGAACATTGACCCCTCCATTGTCATGACCTATGATGCTGTTCAGAATGTGCATTCTGTGTGGACTCTGCGGAGGGTAAAGCCAGAGGAAGAGAATGCTGTTTTAAAGTTCCCTGAGCAGGCAGGGACCCTGCAGAATGCAACTACTAGCAGCTCCCTGACAGCACATCTCAGAAGCCTCTCCAAAGGCGAATCCCCTGTGGCTTCGCCGTTCCAGAATTACTCTTCCATTCACAGCCAGAGTCGCTCAACCTCTTCGCCCAGTCTACACTCTCGGTCGCCTTCCATTTCCAACATGGCGGCTCTAAGTCGTGCTCACTCTCCTGCCTTAGGTGTGCACTCTTTCTCAGGGGCGCAAAGATTCAACCTTTCAAGCCACAGTCAGTCACCAAAGAGACACAGCATTTCTCACTCTCCGAGTGGCAGTTTCAACGACTCATTTTTGGCACCAGAAACAGAGCCAATTGTTCCTGAGCTGTGCATTGATCACTTATGGACTGAGACACTTCCGAATATAAGAGAGAAAAATTCTCAAGCCTCCAAGGTATTTATAACGACTGACCTGTGTGGACAGAAGTTCTTGTGCTTTTTAGTGGAGGCCCAGCTTCAGTTACGCTGTGTAAAGTTTCAAGAGAGTAATGACAAGACTCAGCTTATCTTTGGCTCTGTCACCAACATACATGCAAAAGATGCAGCCCCAGTGGAGAAGATACACACCATGCTGGTCCTAGAAGGCAACGGGAACCTGGTGCTGTACACAGGAGTGGTGCGGGTGGGGAAAGTCTTTATCCCTGGACTTCCGGCTCCTTCCCTGACAATGTCCAACATGATGCCTCGGCCCAGTACCCCGCTCGACGGTGTTGGTACTCCAAAGCCTCTGAGTAAATTGCTTGGATCGATGGATGAGGTGGTTCTGTTGTCTCCAGTTCCAGAATTGAGGGATTCTTCAAAACTTAATGATTCTCTCTACAATGAGGATTGCACATTCCAGCAGCTTGGAACTTACATTCATTCTGTGCGAGACCCTGTTCATAACAGAGTCACTCTAGAACTGAGCAATGGCTCCATGGTTCGGATCACTATCCCTGAAGTGGCCACCTCGGAATTAGTACAAACGTGTCTGCAAGCAATTAAGTTCATCCTGCCAAAAGAAGTAGCCATTCAGGTGCTCGTCAAGTGGTACAATGTGCACAGTGCTCCAGGAGGACCCAGCTGTCACTCAGAGTGGAGTTTATTTGTGATTTGTCTCTTGAACATGATGGGTTATAACACAGACCGCTTAGCATGGACCCGAAGTTTTGACTTTGAAGGATCACTTTCCCCAGTCATTGCACCCAAAAAAGCAAGGCCTTCTGATACTGGATCTGACGAGGACTGGGAGTACTTACTGAACTCAGAGTACCATCGCAATGTTGAGTCTCATCTTTTGAACAAATCTCTATGTTTGACTGCTTTGGAAGTTTCAAATGCAAAGGATGAAGATTTTTCGCAGAACCTCAGTCTGGATTCTTCTACCCTTCTCTTTGCTCACATTCCTGCAATTTTCTTTGTTCTTCACCTGGTTTATGAAGAGCTCAAGTTGAATACTCTAATGGGAGAAGGAATTTGTTCTCTTATCGACCTCCTTGTTCAGTTGGCAAGGGACTTAAAATTGGACTCTTACTTGGACCATTACTATCGAGATTCCCCAACTCTTGTCAAAACCACTGGACAAGTATGCACCATTGACCAAGGTCAGATGGGATTTATGCATCATCCCCCATTTTTTACTTCTGAGCCACCAAGTATTTATCAGTGGGTGAGTTCATGCCTGAAGGGTGAAGGGATGCCACCCTATCCTTACCTTCCTGGGATTTGCGAGAGGAGCAGGCTAGTGGTCTTGAGCATTGCCCTCTATACCCTTGGTGATGAGAGCTGTGTGTCTGATGAAACTTGCCAGTACTTATCCAAAGTAACCTCAACTCCCCAAAAGCCACAAGCAGAGCAAGAAGAAAACAGGTTTACTTTCCGGCATTCTGCTTCCGTTTCTGTTCTGGCCGAGAGATTAGTTGTCTGGATGGCCAGTGTAGGATTCACTTTAAGAGATCTGGAGACTCTTCCCTTTGGGATTGCTCTTCCCATCAGAGATGCGATTTACCACTGTCGGGAGCAGCCTGATTCAGATTGGTCAGAAGCGGTCTGTCTCTTGATTGGACGTCAGGATCTTTCCAAGCAGGCTTGTGAAGGAAATTTACCCAGAGGCAAATCTGTGCTCTCATCAGAGGTGTCTTCAGGAACTGAGGCAGAGGAGGAAGATGATGGCATGAATGACTTGAACCATGAGGTTATGTCATTAATATGGAGTGAAGATTTACGGGTGCAGGATGTGCGAAGGCTGCTTCAAAGTGCACAGCCTGTCCGTGTCAATGTGGTGCAGTACCCAGAACTCAGTGACCATGAGTTCATTGAAGAAAAGGAAAACAGACTGCTCCAGTTGTGTCAGCGAACTATGGCCCTTCCAGTAGGACGAGGAATGTTTACCTTGTTCTCATATCATCCTGTTCCAACAGAGCCGTTGCCTGTTCCTAAATTGAATCTGACAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCCTTCCAGTAGGACGAGGA
60.03
AAATCCCCGGCTGCAATCAT
60.4
164
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CCCTTCCAGTAGGACGAGGA
60.03
AATCCCCGGCTGCAATCATC
60.83
163
PCR template sequence:
CGAACTATGGCCCTTCCAGTAGGACGAGGAATGTTTACCTTGTTCTCATATCATCCTGTTCCAACAGAGCCGTTGCCTGTTCCTAAATTGAATCTGACAGAAATAATGTTCATATTTGGAACCCATGTCGAACTTCTCTGAAGAAAGGGCAACGATGATTGCAGCCGGGGATTTGCAGGAATTTGTTCCTTTTGGCCGAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
21
Interacting miRNAs (#binding sites)
mmu-miR-9-5p(1), mmu-miR-140-5p(1), mmu-miR-134-5p(1), mmu-miR-206-3p(1), mmu-miR-103-3p(1), mmu-miR-135a-5p(1), mmu-miR-30e-5p(1), mmu-miR-322-5p(1), mmu-miR-30a-5p(1), mmu-miR-30c-5p(1), mmu-miR-216a-5p(1), mmu-miR-326-3p(1), mmu-miR-30d-5p(1), mmu-miR-30b-5p(1), mmu-miR-351-5p(1), mmu-miR-107-3p(1), mmu-miR-539-5p(1), mmu-miR-697(1), mmu-miR-99a-5p(1), mmu-miR-338-3p(1), mmu-miR-7a-1-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TTGAATCTGACAGAAATAATGTTCAT
siRNA sets
ATTATTTCTGTCAGATTCA, TTATTTCTGTCAGATTCAA, ATGAACATTATTTCTGTCA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCANAPC1_3349_ORF_1
0.8309
0.0992988
0.999999999
3366
ORF sequence:
ATGTCGAACTTCTCTGAAGAAAGGGCAACGATGATTGCAGCCGGGGATTTGCAGGAATTTGTTCCTTTTGGCCGAGACCACTGCAAGCACCACCCTAACGCTCTGAACCTTCAGCTTCGTCAGCTGCAGCCAGCCTCTGAGTTATGGTCTTCTGATGGTGCAGCTGGCTTGGTGGGATCTCTTCAGGAGGTTACAATCCATGAGAAACAGAAGGAAAGCTGGCAGTTAAGAAAAGGTGTGAGTGAGATTGGAGATGCAGCTGACTACGATGAAGAGCTCTATGTGGCTGGGAACATGGTTATCTGGAGCAAAGGGAGTAAAAGCCAGGCACTGGCAGTGTACAAAGCGTTCACAGTGGACAGCACTGTCCAGCAGGCATTGTGGTGTGACTTCATTATATCACAGGATAAGTCTGAAAAGATCTATAAGAGCCATGAACTAGAGAAATGCATATGTATATTACAGAGTTCATGTATGAACATGCATAGCATAGACGGGAAGGATTATATAGCTTCCTTACCTTTTCAGGTGGCAAATGTTTGGGCCACTAAATATGGCTTGCTGTTTGAACGATGCAGTTCTTCACATGAGGTACCTCCAAGTCTACCTAGAGAACCTTTGCCTACTATGTTCAGCATGCTGCACCCACTGGATGAGATAACTCCACTTGTTTGTAAATCCGGAAGTCTTTTTGGCTCATCACGGGTACAGTATGTTGTAGATCCAGCAGTGAAAATTGTGTTTCTGAACATTGACCCCTCCATTGTCATGACCTATGATGCTGTTCAGAATGTGCATTCTGTGTGGACTCTGCGGAGGGTAAAGCCAGAGGAAGAGAATGCTGTTTTAAAGTTCCCTGAGCAGGCAGGGACCCTGCAGAATGCAACTACTAGCAGCTCCCTGACAGCACATCTCAGAAGCCTCTCCAAAGGCGAATCCCCTGTGGCTTCGCCGTTCCAGAATTACTCTTCCATTCACAGCCAGAGTCGCTCAACCTCTTCGCCCAGTCTACACTCTCGGTCGCCTTCCATTTCCAACATGGCGGCTCTAAGTCGTGCTCACTCTCCTGCCTTAGGTGTGCACTCTTTCTCAGGGGCGCAAAGATTCAACCTTTCAAGCCACAGTCAGTCACCAAAGAGACACAGCATTTCTCACTCTCCGAGTGGCAGTTTCAACGACTCATTTTTGGCACCAGAAACAGAGCCAATTGTTCCTGAGCTGTGCATTGATCACTTATGGACTGAGACACTTCCGAATATAAGAGAGAAAAATTCTCAAGCCTCCAAGGTATTTATAACGACTGACCTGTGTGGACAGAAGTTCTTGTGCTTTTTAGTGGAGGCCCAGCTTCAGTTACGCTGTGTAAAGTTTCAAGAGAGTAATGACAAGACTCAGCTTATCTTTGGCTCTGTCACCAACATACATGCAAAAGATGCAGCCCCAGTGGAGAAGATACACACCATGCTGGTCCTAGAAGGCAACGGGAACCTGGTGCTGTACACAGGAGTGGTGCGGGTGGGGAAAGTCTTTATCCCTGGACTTCCGGCTCCTTCCCTGACAATGTCCAACATGATGCCTCGGCCCAGTACCCCGCTCGACGGTGTTGGTACTCCAAAGCCTCTGAGTAAATTGCTTGGATCGATGGATGAGGTGGTTCTGTTGTCTCCAGTTCCAGAATTGAGGGATTCTTCAAAACTTAATGATTCTCTCTACAATGAGGATTGCACATTCCAGCAGCTTGGAACTTACATTCATTCTGTGCGAGACCCTGTTCATAACAGAGTCACTCTAGAACTGAGCAATGGCTCCATGGTTCGGATCACTATCCCTGAAGTGGCCACCTCGGAATTAGTACAAACGTGTCTGCAAGCAATTAAGTTCATCCTGCCAAAAGAAGTAGCCATTCAGGTGCTCGTCAAGTGGTACAATGTGCACAGTGCTCCAGGAGGACCCAGCTGTCACTCAGAGTGGAGTTTATTTGTGATTTGTCTCTTGAACATGATGGGTTATAACACAGACCGCTTAGCATGGACCCGAAGTTTTGACTTTGAAGGATCACTTTCCCCAGTCATTGCACCCAAAAAAGCAAGGCCTTCTGATACTGGATCTGACGAGGACTGGGAGTACTTACTGAACTCAGAGTACCATCGCAATGTTGAGTCTCATCTTTTGAACAAATCTCTATGTTTGACTGCTTTGGAAGTTTCAAATGCAAAGGATGAAGATTTTTCGCAGAACCTCAGTCTGGATTCTTCTACCCTTCTCTTTGCTCACATTCCTGCAATTTTCTTTGTTCTTCACCTGGTTTATGAAGAGCTCAAGTTGAATACTCTAATGGGAGAAGGAATTTGTTCTCTTATCGACCTCCTTGTTCAGTTGGCAAGGGACTTAAAATTGGACTCTTACTTGGACCATTACTATCGAGATTCCCCAACTCTTGTCAAAACCACTGGACAAGTATGCACCATTGACCAAGGTCAGATGGGATTTATGCATCATCCCCCATTTTTTACTTCTGAGCCACCAAGTATTTATCAGTGGGTGAGTTCATGCCTGAAGGGTGAAGGGATGCCACCCTATCCTTACCTTCCTGGGATTTGCGAGAGGAGCAGGCTAGTGGTCTTGAGCATTGCCCTCTATACCCTTGGTGATGAGAGCTGTGTGTCTGATGAAACTTGCCAGTACTTATCCAAAGTAACCTCAACTCCCCAAAAGCCACAAGCAGAGCAAGAAGAAAACAGGTTTACTTTCCGGCATTCTGCTTCCGTTTCTGTTCTGGCCGAGAGATTAGTTGTCTGGATGGCCAGTGTAGGATTCACTTTAAGAGATCTGGAGACTCTTCCCTTTGGGATTGCTCTTCCCATCAGAGATGCGATTTACCACTGTCGGGAGCAGCCTGATTCAGATTGGTCAGAAGCGGTCTGTCTCTTGATTGGACGTCAGGATCTTTCCAAGCAGGCTTGTGAAGGAAATTTACCCAGAGGCAAATCTGTGCTCTCATCAGAGGTGTCTTCAGGAACTGAGGCAGAGGAGGAAGATGATGGCATGAATGACTTGAACCATGAGGTTATGTCATTAATATGGAGTGAAGATTTACGGGTGCAGGATGTGCGAAGGCTGCTTCAAAGTGCACAGCCTGTCCGTGTCAATGTGGTGCAGTACCCAGAACTCAGTGACCATGAGTTCATTGAAGAAAAGGAAAACAGACTGCTCCAGTTGTGTCAGCGAACTATGGCCCTTCCAGTAGGACGAGGAATGTTTACCTTGTTCTCATATCATCCTGTTCCAACAGAGCCGTTGCCTGTTCCTAAATTGAATCTGACAGAAATAATGTTCATATTTGGAACCCATGTCGAACTTCTCTGA
Peptide sequence:
MSNFSEERATMIAAGDLQEFVPFGRDHCKHHPNALNLQLRQLQPASELWSSDGAAGLVGSLQEVTIHEKQKESWQLRKGVSEIGDAADYDEELYVAGNMVIWSKGSKSQALAVYKAFTVDSTVQQALWCDFIISQDKSEKIYKSHELEKCICILQSSCMNMHSIDGKDYIASLPFQVANVWATKYGLLFERCSSSHEVPPSLPREPLPTMFSMLHPLDEITPLVCKSGSLFGSSRVQYVVDPAVKIVFLNIDPSIVMTYDAVQNVHSVWTLRRVKPEEENAVLKFPEQAGTLQNATTSSSLTAHLRSLSKGESPVASPFQNYSSIHSQSRSTSSPSLHSRSPSISNMAALSRAHSPALGVHSFSGAQRFNLSSHSQSPKRHSISHSPSGSFNDSFLAPETEPIVPELCIDHLWTETLPNIREKNSQASKVFITTDLCGQKFLCFLVEAQLQLRCVKFQESNDKTQLIFGSVTNIHAKDAAPVEKIHTMLVLEGNGNLVLYTGVVRVGKVFIPGLPAPSLTMSNMMPRPSTPLDGVGTPKPLSKLLGSMDEVVLLSPVPELRDSSKLNDSLYNEDCTFQQLGTYIHSVRDPVHNRVTLELSNGSMVRITIPEVATSELVQTCLQAIKFILPKEVAIQVLVKWYNVHSAPGGPSCHSEWSLFVICLLNMMGYNTDRLAWTRSFDFEGSLSPVIAPKKARPSDTGSDEDWEYLLNSEYHRNVESHLLNKSLCLTALEVSNAKDEDFSQNLSLDSSTLLFAHIPAIFFVLHLVYEELKLNTLMGEGICSLIDLLVQLARDLKLDSYLDHYYRDSPTLVKTTGQVCTIDQGQMGFMHHPPFFTSEPPSIYQWVSSCLKGEGMPPYPYLPGICERSRLVVLSIALYTLGDESCVSDETCQYLSKVTSTPQKPQAEQEENRFTFRHSASVSVLAERLVVWMASVGFTLRDLETLPFGIALPIRDAIYHCREQPDSDWSEAVCLLIGRQDLSKQACEGNLPRGKSVLSSEVSSGTEAEEEDDGMNDLNHEVMSLIWSEDLRVQDVRRLLQSAQPVRVNVVQYPELSDHEFIEEKENRLLQLCQRTMALPVGRGMFTLFSYHPVPTEPLPVPKLNLTEIMFIFGTHVELL
IRESfinder result
IRESfinder score
0.682025008
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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