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mmu_circAnapc1_2064
Basic information of circular RNA
PanCircBase ID:
mmu_circAnapc1_2064
mm39_Chr. Position ID:
chr2|128505794|128527980|-
Gene name:
Anapc1
Transcript:
ENSMUST00000014499.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
16
Exon/intron indexes:
17,16,15,14,13,12,11,10,9,8,7,6,5,4,3,2
Exon/intron offsets:
0,1573,2690,5050,5775,5948,8187,10676,11796,12227,14336,15645,16234,16519,20627,21949
Genomic length:
22186
Exon/intron sizes in nt:
188,61,141,135,66,91,96,210,221,146,74,83,101,52,162,237
Flanking exon/introns:
chr2:128502799-128505794|chr2:128527980-128529072
Splice length:
2064
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
AAATAATGTTCATATTTGGAACCCATGTCGAACTTCTCTGAAGAAAGGGCAACGATGATTGCAGCCGGGGATTTGCAGGAATTTGTTCCTTTTGGCCGAGACCACTGCAAGCACCACCCTAACGCTCTGAACCTTCAGCTTCGTCAGCTGCAGCCAGCCTCTGAGTTATGGTCTTCTGATGGTGCAGCTGGCTTGGTGGGATCTCTTCAGGAGGTTACAATCCATGAGAAACAGAAGGAAAGCTGGCAGTTAAGAAAAGGTGTGAGTGAGATTGGAGATGCAGCTGACTACGATGAAGAGCTCTATGTGGCTGGGAACATGGTTATCTGGAGCAAAGGGAGTAAAAGCCAGGCACTGGCAGTGTACAAAGCGTTCACAGTGGACAGCACTGTCCAGCAGGCATTGTGGTGTGACTTCATTATATCACAGGATAAGTCTGAAAAGATCTATAAGAGCCATGAACTAGAGAAATGCATATGTATATTACAGAGTTCATGTATGAACATGCATAGCATAGACGGGAAGGATTATATAGCTTCCTTACCTTTTCAGGTGGCAAATGTTTGGGCCACTAAATATGGCTTGCTGTTTGAACGATGCAGTTCTTCACATGAGGTACCTCCAAGTCTACCTAGAGAACCTTTGCCTACTATGTTCAGCATGCTGCACCCACTGGATGAGATAACTCCACTTGTTTGTAAATCCGGAAGTCTTTTTGGCTCATCACGGGTACAGTATGTTGTAGATCCAGCAGTGAAAATTGTGTTTCTGAACATTGACCCCTCCATTGTCATGACCTATGATGCTGTTCAGAATGTGCATTCTGTGTGGACTCTGCGGAGGGTAAAGCCAGAGGAAGAGAATGCTGTTTTAAAGTTCCCTGAGCAGGCAGGGACCCTGCAGAATGCAACTACTAGCAGCTCCCTGACAGCACATCTCAGAAGCCTCTCCAAAGGCGAATCCCCTGTGGCTTCGCCGTTCCAGAATTACTCTTCCATTCACAGCCAGAGTCGCTCAACCTCTTCGCCCAGTCTACACTCTCGGTCGCCTTCCATTTCCAACATGGCGGCTCTAAGTCGTGCTCACTCTCCTGCCTTAGGTGTGCACTCTTTCTCAGGGGCGCAAAGATTCAACCTTTCAAGCCACAGTCAGTCACCAAAGAGACACAGCATTTCTCACTCTCCGAGTGGCAGTTTCAACGACTCATTTTTGGCACCAGAAACAGAGCCAATTGTTCCTGAGCTGTGCATTGATCACTTATGGACTGAGACACTTCCGAATATAAGAGAGAAAAATTCTCAAGCCTCCAAGGTATTTATAACGACTGACCTGTGTGGACAGAAGTTCTTGTGCTTTTTAGTGGAGGCCCAGCTTCAGTTACGCTGTGTAAAGTTTCAAGAGAGTAATGACAAGACTCAGCTTATCTTTGGCTCTGTCACCAACATACATGCAAAAGATGCAGCCCCAGTGGAGAAGATACACACCATGCTGGTCCTAGAAGGCAACGGGAACCTGGTGCTGTACACAGGAGTGGTGCGGGTGGGGAAAGTCTTTATCCCTGGACTTCCGGCTCCTTCCCTGACAATGTCCAACATGATGCCTCGGCCCAGTACCCCGCTCGACGGTGTTGGTACTCCAAAGCCTCTGAGTAAATTGCTTGGATCGATGGATGAGGTGGTTCTGTTGTCTCCAGTTCCAGAATTGAGGGATTCTTCAAAACTTAATGATTCTCTCTACAATGAGGATTGCACATTCCAGCAGCTTGGAACTTACATTCATTCTGTGCGAGACCCTGTTCATAACAGAGTCACTCTAGAACTGAGCAATGGCTCCATGGTTCGGATCACTATCCCTGAAGTGGCCACCTCGGAATTAGTACAAACGTGTCTGCAAGCAATTAAGTTCATCCTGCCAAAAGAAGTAGCCATTCAGGTGCTCGTCAAGTGGTACAATGTGCACAGTGCTCCAGGAGGACCCAGCTGTCACTCAGAGTGGAGTTTATTTGTGATTTGTCTCTTGAACATGATGGGTTATAACACAGACCGCTTAGCATGGACCCGAAGT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCAGCTGTCACTCAGAGTGG
60.04
AAATCCCCGGCTGCAATCAT
60.4
163
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GACCCAGCTGTCACTCAGAG
59.75
AAATCCCCGGCTGCAATCAT
60.4
166
PCR template sequence:
TCCAGGAGGACCCAGCTGTCACTCAGAGTGGAGTTTATTTGTGATTTGTCTCTTGAACATGATGGGTTATAACACAGACCGCTTAGCATGGACCCGAAGTAAATAATGTTCATATTTGGAACCCATGTCGAACTTCTCTGAAGAAAGGGCAACGATGATTGCAGCCGGGGATTTGCAGGAATTTGTTCCTTTTGGCCGAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
10
Interacting miRNAs (#binding sites)
mmu-miR-9-5p(1), mmu-miR-134-5p(1), mmu-miR-103-3p(1), mmu-miR-135a-5p(1), mmu-miR-322-5p(1), mmu-miR-216a-5p(1), mmu-miR-351-5p(1), mmu-miR-107-3p(1), mmu-miR-539-5p(1), mmu-miR-338-3p(1)
siRNAs for circular RNA silencing
Junction sequence
ATGGACCCGAAGTAAATAATGTTCAT
siRNA sets
ATTATTTACTTCGGGTCCA, CATTATTTACTTCGGGTCC, TGAACATTATTTACTTCGG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCANAPC1_2064_ORF_1
0.8401
0.12031461
0.999989657
2046
ORF sequence:
ATGTCGAACTTCTCTGAAGAAAGGGCAACGATGATTGCAGCCGGGGATTTGCAGGAATTTGTTCCTTTTGGCCGAGACCACTGCAAGCACCACCCTAACGCTCTGAACCTTCAGCTTCGTCAGCTGCAGCCAGCCTCTGAGTTATGGTCTTCTGATGGTGCAGCTGGCTTGGTGGGATCTCTTCAGGAGGTTACAATCCATGAGAAACAGAAGGAAAGCTGGCAGTTAAGAAAAGGTGTGAGTGAGATTGGAGATGCAGCTGACTACGATGAAGAGCTCTATGTGGCTGGGAACATGGTTATCTGGAGCAAAGGGAGTAAAAGCCAGGCACTGGCAGTGTACAAAGCGTTCACAGTGGACAGCACTGTCCAGCAGGCATTGTGGTGTGACTTCATTATATCACAGGATAAGTCTGAAAAGATCTATAAGAGCCATGAACTAGAGAAATGCATATGTATATTACAGAGTTCATGTATGAACATGCATAGCATAGACGGGAAGGATTATATAGCTTCCTTACCTTTTCAGGTGGCAAATGTTTGGGCCACTAAATATGGCTTGCTGTTTGAACGATGCAGTTCTTCACATGAGGTACCTCCAAGTCTACCTAGAGAACCTTTGCCTACTATGTTCAGCATGCTGCACCCACTGGATGAGATAACTCCACTTGTTTGTAAATCCGGAAGTCTTTTTGGCTCATCACGGGTACAGTATGTTGTAGATCCAGCAGTGAAAATTGTGTTTCTGAACATTGACCCCTCCATTGTCATGACCTATGATGCTGTTCAGAATGTGCATTCTGTGTGGACTCTGCGGAGGGTAAAGCCAGAGGAAGAGAATGCTGTTTTAAAGTTCCCTGAGCAGGCAGGGACCCTGCAGAATGCAACTACTAGCAGCTCCCTGACAGCACATCTCAGAAGCCTCTCCAAAGGCGAATCCCCTGTGGCTTCGCCGTTCCAGAATTACTCTTCCATTCACAGCCAGAGTCGCTCAACCTCTTCGCCCAGTCTACACTCTCGGTCGCCTTCCATTTCCAACATGGCGGCTCTAAGTCGTGCTCACTCTCCTGCCTTAGGTGTGCACTCTTTCTCAGGGGCGCAAAGATTCAACCTTTCAAGCCACAGTCAGTCACCAAAGAGACACAGCATTTCTCACTCTCCGAGTGGCAGTTTCAACGACTCATTTTTGGCACCAGAAACAGAGCCAATTGTTCCTGAGCTGTGCATTGATCACTTATGGACTGAGACACTTCCGAATATAAGAGAGAAAAATTCTCAAGCCTCCAAGGTATTTATAACGACTGACCTGTGTGGACAGAAGTTCTTGTGCTTTTTAGTGGAGGCCCAGCTTCAGTTACGCTGTGTAAAGTTTCAAGAGAGTAATGACAAGACTCAGCTTATCTTTGGCTCTGTCACCAACATACATGCAAAAGATGCAGCCCCAGTGGAGAAGATACACACCATGCTGGTCCTAGAAGGCAACGGGAACCTGGTGCTGTACACAGGAGTGGTGCGGGTGGGGAAAGTCTTTATCCCTGGACTTCCGGCTCCTTCCCTGACAATGTCCAACATGATGCCTCGGCCCAGTACCCCGCTCGACGGTGTTGGTACTCCAAAGCCTCTGAGTAAATTGCTTGGATCGATGGATGAGGTGGTTCTGTTGTCTCCAGTTCCAGAATTGAGGGATTCTTCAAAACTTAATGATTCTCTCTACAATGAGGATTGCACATTCCAGCAGCTTGGAACTTACATTCATTCTGTGCGAGACCCTGTTCATAACAGAGTCACTCTAGAACTGAGCAATGGCTCCATGGTTCGGATCACTATCCCTGAAGTGGCCACCTCGGAATTAGTACAAACGTGTCTGCAAGCAATTAAGTTCATCCTGCCAAAAGAAGTAGCCATTCAGGTGCTCGTCAAGTGGTACAATGTGCACAGTGCTCCAGGAGGACCCAGCTGTCACTCAGAGTGGAGTTTATTTGTGATTTGTCTCTTGAACATGATGGGTTATAACACAGACCGCTTAGCATGGACCCGAAGTAAATAA
Peptide sequence:
MSNFSEERATMIAAGDLQEFVPFGRDHCKHHPNALNLQLRQLQPASELWSSDGAAGLVGSLQEVTIHEKQKESWQLRKGVSEIGDAADYDEELYVAGNMVIWSKGSKSQALAVYKAFTVDSTVQQALWCDFIISQDKSEKIYKSHELEKCICILQSSCMNMHSIDGKDYIASLPFQVANVWATKYGLLFERCSSSHEVPPSLPREPLPTMFSMLHPLDEITPLVCKSGSLFGSSRVQYVVDPAVKIVFLNIDPSIVMTYDAVQNVHSVWTLRRVKPEEENAVLKFPEQAGTLQNATTSSSLTAHLRSLSKGESPVASPFQNYSSIHSQSRSTSSPSLHSRSPSISNMAALSRAHSPALGVHSFSGAQRFNLSSHSQSPKRHSISHSPSGSFNDSFLAPETEPIVPELCIDHLWTETLPNIREKNSQASKVFITTDLCGQKFLCFLVEAQLQLRCVKFQESNDKTQLIFGSVTNIHAKDAAPVEKIHTMLVLEGNGNLVLYTGVVRVGKVFIPGLPAPSLTMSNMMPRPSTPLDGVGTPKPLSKLLGSMDEVVLLSPVPELRDSSKLNDSLYNEDCTFQQLGTYIHSVRDPVHNRVTLELSNGSMVRITIPEVATSELVQTCLQAIKFILPKEVAIQVLVKWYNVHSAPGGPSCHSEWSLFVICLLNMMGYNTDRLAWTRSK
IRESfinder result
IRESfinder score
0.630692323
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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