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mmu_circAldh7a1_485
Basic information of circular RNA
PanCircBase ID:
mmu_circAldh7a1_485
mm39_Chr. Position ID:
chr18|56692293|56695747|-
Gene name:
Aldh7a1
Transcript:
ENSMUST00000174518.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
5,4,3,2
Exon/intron offsets:
0,1554,2811,3261
Genomic length:
3454
Exon/intron sizes in nt:
6,65,41,72,193
Flanking exon/introns:
chr18:56684312-56692293|chr18:56695747-56705916
Splice length:
485
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTCCCAGCCCAGATCCAAGGAACGAGCATGCCCAGAATACCTGAGCTCTGAGCGTAGTGCGGCCCAGCAGCGTGCGTCGGTTTGAAAGCTCGGCTCCAACCCAGCGGACTTCAGAAGCAGCGGGTGGCGCAGTTCCCCGCCCCCTCAGCCTTGTCCCCGCCCCAGACCCTGAGGCAGCAACTTTACCCAGAGGGCTCTGTGTGCAGTCTGTGAAGACCAGCAAGCTTTCCGGACCTTGGAGCAGGCCCGCCGCCCACATGTCTACTCTGCTGATCCATCATCCCCAGTATGCCTGGCTGCAAGACCTGGGGCTCCGCGAGGATAACGAGGGCGTGTATAATGGAAGCTGGGGCGGCCGGGGAGAGGTAATTACGACCTATTGTCCTGCTAACAATGAGCCAATAGCAAGAGTCCGACAGGCCAGCCTGAAGGACTATGAAGAAACCATCGGGAAAGCCAAGAAAGCCTGGAACATCTGGGCAGAT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ATAGCAAGAGTCCGACAGGC
59.54
CACTACGCTCAGAGCTCAGG
59.9
143
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGCCAGCCTGAAGGACTATG
60.18
GAGCTTTCAAACCGACGCAC
60.38
158
PCR template sequence:
TGCTAACAATGAGCCAATAGCAAGAGTCCGACAGGCCAGCCTGAAGGACTATGAAGAAACCATCGGGAAAGCCAAGAAAGCCTGGAACATCTGGGCAGATGTCCCAGCCCAGATCCAAGGAACGAGCATGCCCAGAATACCTGAGCTCTGAGCGTAGTGCGGCCCAGCAGCGTGCGTCGGTTTGAAAGCTCGGCTCCAAC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-431-5p(1), mmu-miR-29b-2-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CATCTGGGCAGATGTCCCAGCCCAGA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCALDH7A1_485_ORF_1
0.9006
0.296432598
0.40958752
279
ORF sequence:
ATGTCTACTCTGCTGATCCATCATCCCCAGTATGCCTGGCTGCAAGACCTGGGGCTCCGCGAGGATAACGAGGGCGTGTATAATGGAAGCTGGGGCGGCCGGGGAGAGGTAATTACGACCTATTGTCCTGCTAACAATGAGCCAATAGCAAGAGTCCGACAGGCCAGCCTGAAGGACTATGAAGAAACCATCGGGAAAGCCAAGAAAGCCTGGAACATCTGGGCAGATGTCCCAGCCCAGATCCAAGGAACGAGCATGCCCAGAATACCTGAGCTCTGA
Peptide sequence:
MSTLLIHHPQYAWLQDLGLREDNEGVYNGSWGGRGEVITTYCPANNEPIARVRQASLKDYEETIGKAKKAWNIWADVPAQIQGTSMPRIPEL
IRESfinder result
IRESfinder score
0.320260226
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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