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mmu_circAldh7a1_358
Basic information of circular RNA
PanCircBase ID:
mmu_circAldh7a1_358
mm39_Chr. Position ID:
chr18|56670275|56680020|-
Gene name:
Aldh7a1
Transcript:
ENSMUST00000174518.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
13,12,11,10,9
Exon/intron offsets:
0,1497,5021,7761,9700
Genomic length:
9745
Exon/intron sizes in nt:
95,42,98,78,45
Flanking exon/introns:
chr18:56667346-56670275|chr18:56680020-56681518
Splice length:
358
Other database IDs:
circAtlas: mmu-Aldh7a1_0022
Human Ortholog IDs:
Mature spliced Sequence:
GAAAGGAGCACCGACTACGTCCCTCGTTAGTGTGGCTGTCACAAAGATCATAGCCCAGGTTTTGGAGGACAACCTGCTGCCCGGTGCCATTTGTTCCCTGGTTTGTGGTGGAGCAGATATCGGCACAACGATGGCCAGAGATGAGCGTGTGAACCTGCTGTCCTTCACTGGGAGCACTCAGGTGGGGAAGGAGGTGGCCCTCATGGTGCAGGAGAGGTTTGGGAAAAGCTTGTTGGAGCTTGGAGGAAACAATGCCATTATTGCTTTCGAGGACGCGGACCTCAGCTTGGTTGTTCCGTCAGTTCTGTTTGCCGCCGTGGGAACAGCTGGGCAAAGGTGTACCACTGTGAGGAGACTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCAGCTTGGTTGTTCCGTCA
59.82
TTGTCCTCCAAAACCTGGGC
60.47
149
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGCTTGGTTGTTCCGTCAGT
59.82
TTGTCCTCCAAAACCTGGGC
60.47
147
PCR template sequence:
TATTGCTTTCGAGGACGCGGACCTCAGCTTGGTTGTTCCGTCAGTTCTGTTTGCCGCCGTGGGAACAGCTGGGCAAAGGTGTACCACTGTGAGGAGACTGGAAAGGAGCACCGACTACGTCCCTCGTTAGTGTGGCTGTCACAAAGATCATAGCCCAGGTTTTGGAGGACAACCTGCTGCCCGGTGCCATTTGTTCCCTG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-27b-3p(1), mmu-miR-27a-3p(1), mmu-miR-214-3p(1), mmu-miR-183-5p(1), mmu-miR-7a-1-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TGTGAGGAGACTGGAAAGGAGCACCG
siRNA sets
GTGCTCCTTTCCAGTCTCC, TGCTCCTTTCCAGTCTCCT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCALDH7A1_358_ORF_1
1.1454
0.258029305
0.446943339
258
ORF sequence:
ATGGCCAGAGATGAGCGTGTGAACCTGCTGTCCTTCACTGGGAGCACTCAGGTGGGGAAGGAGGTGGCCCTCATGGTGCAGGAGAGGTTTGGGAAAAGCTTGTTGGAGCTTGGAGGAAACAATGCCATTATTGCTTTCGAGGACGCGGACCTCAGCTTGGTTGTTCCGTCAGTTCTGTTTGCCGCCGTGGGAACAGCTGGGCAAAGGTGTACCACTGTGAGGAGACTGGAAAGGAGCACCGACTACGTCCCTCGTTAG
Peptide sequence:
MARDERVNLLSFTGSTQVGKEVALMVQERFGKSLLELGGNNAIIAFEDADLSLVVPSVLFAAVGTAGQRCTTVRRLERSTDYVPR
IRESfinder result
IRESfinder score
0.41568692
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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