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mmu_circAkt3_132
Basic information of circular RNA
PanCircBase ID:
mmu_circAkt3_132
mm39_Chr. Position ID:
chr1|176924532|176924664|-
Gene name:
Akt3
Transcript:
ENSMUST00000019843.15
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
1
Exon/intron indexes:
5
Exon/intron offsets:
0
Genomic length:
132
Exon/intron sizes in nt:
132
Flanking exon/introns:
chr1:176907832-176924532|chr1:176924664-176930527
Splice length:
132
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
ACGATGAATGATTTTGACTATTTGAAACTACTAGGTAAAGGCACTTTTGGGAAAGTTATTTTGGTTCGAGAGAAGGCAAGTGGAAAATACTATGCTATGAAGATTCTGAAGAAAGAAGTCATTATTGCAAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CGAGAGAAGGCAAGTGGAAA
57.83
CTTTCCCAAAAGTGCCTTTACCT
59.36
121
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CGAGAGAAGGCAAGTGGAAA
57.83
TTTCCCAAAAGTGCCTTTACCT
58.35
120
PCR template sequence:
CGAGAGAAGGCAAGTGGAAAATACTATGCTATGAAGATTCTGAAGAAAGAAGTCATTATTGCAAAGACGATGAATGATTTTGACTATTTGAAACTACTAGGTAAAGGCACTTTTGGGAAAGTTATTTTGGTT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-93-5p(1), mmu-miR-20a-5p(1), mmu-miR-17-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CATTATTGCAAAGACGATGAATGATT
siRNA sets
CATCGTCTTTGCAATAATG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCAKT3_132_ORF_1
1.059
0.154847534
0.317917344
261
ORF sequence:
ATGAATGATTTTGACTATTTGAAACTACTAGGTAAAGGCACTTTTGGGAAAGTTATTTTGGTTCGAGAGAAGGCAAGTGGAAAATACTATGCTATGAAGATTCTGAAGAAAGAAGTCATTATTGCAAAGACGATGAATGATTTTGACTATTTGAAACTACTAGGTAAAGGCACTTTTGGGAAAGTTATTTTGGTTCGAGAGAAGGCAAGTGGAAAATACTATGCTATGAAGATTCTGAAGAAAGAAGTCATTATTGCAAAG
Peptide sequence:
MNDFDYLKLLGKGTFGKVILVREKASGKYYAMKILKKEVIIAKTMNDFDYLKLLGKGTFGKVILVREKASGKYYAMKILKKEVIIAK
IRESfinder result
IRESfinder score
0.598781973
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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