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mmu_circAkt2_241
Basic information of circular RNA
PanCircBase ID:
mmu_circAkt2_241
mm39_Chr. Position ID:
chr7|27315665|27317744|+
Gene name:
Akt2
Transcript:
ENSMUST00000108344.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
3,4
Exon/intron offsets:
0,1967
Genomic length:
2079
Exon/intron sizes in nt:
1,29,112
Flanking exon/introns:
chr7:27308178-27315665|chr7:27317744-27328261
Splice length:
241
Other database IDs:
circBase: hsa_circ_0051081, circAtlas: hsa-AKT2_0002, CIRCpedia: HSA_CIRCpedia_25928
Human Ortholog IDs:
Mature spliced Sequence:
GTGAATACATCAAGACCTGGAGGCCACGGTACTTCCTTCTGAAGAGTGATGGATCTTTCATTGGGTATAAGGAGAGGCCCGAGGCCCCTGACCAGACCTTACCCCCCCTGAACAATTTCTCTGTAGCAGAATGCCAGCTGATGAAGACTGAGAGGCCACGACCCAACACCTTTGTCATACGCTGCCTGCAGTGGACCACAGTCATCGAGAGGACCTTCCATGTAGACTCTCCAGATGAGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CTTTGTCATACGCTGCCTGC
59.9
TCGGGCCTCTCCTTATACCC
60.18
154
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACGACCCAACACCTTTGTCA
59.75
TCGGGCCTCTCCTTATACCC
60.18
166
PCR template sequence:
TGAAGACTGAGAGGCCACGACCCAACACCTTTGTCATACGCTGCCTGCAGTGGACCACAGTCATCGAGAGGACCTTCCATGTAGACTCTCCAGATGAGAGGTGAATACATCAAGACCTGGAGGCCACGGTACTTCCTTCTGAAGAGTGATGGATCTTTCATTGGGTATAAGGAGAGGCCCGAGGCCCCTGACCAGACCTT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-382-5p(1), mmu-miR-539-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CTCCAGATGAGAGGTGAATACATCAA
siRNA sets
TATTCACCTCTCATCTGGA,TTGATGTATTCACCTCTCA,TGTATTCACCTCTCATCTG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCAKT2_241_ORF_1
0.9397
0.28646542
0.363444947
246
ORF sequence:
ATGAGAGGTGAATACATCAAGACCTGGAGGCCACGGTACTTCCTTCTGAAGAGTGATGGATCTTTCATTGGGTATAAGGAGAGGCCCGAGGCCCCTGACCAGACCTTACCCCCCCTGAACAATTTCTCTGTAGCAGAATGCCAGCTGATGAAGACTGAGAGGCCACGACCCAACACCTTTGTCATACGCTGCCTGCAGTGGACCACAGTCATCGAGAGGACCTTCCATGTAGACTCTCCAGATGAG
Peptide sequence:
MRGEYIKTWRPRYFLLKSDGSFIGYKERPEAPDQTLPPLNNFSVAECQLMKTERPRPNTFVIRCLQWTTVIERTFHVDSPDE
IRESfinder result
IRESfinder score
0.571869832
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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