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mmu_circAkap9_1365
Basic information of circular RNA
PanCircBase ID:
mmu_circAkap9_1365
mm39_Chr. Position ID:
chr5|4114070|4119207|+
Gene name:
Akap9
Transcript:
ENSMUST00000044492.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
39,40,41,42,43
Exon/intron offsets:
0,1110,1734,3669,4936
Genomic length:
5137
Exon/intron sizes in nt:
66,92,06,10,61,83,201
Flanking exon/introns:
chr5:4111288-4114070|chr5:4119207-4120298
Splice length:
1365
Other database IDs:
circBase: hsa_circ_0135035, circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_118214
Human Ortholog IDs:
Mature spliced Sequence:
GATCTGAAATTTTCACTTGAGGATCAGAAACGGAGAAATACACAGTTAAACCTCCTTTTGGAACAACAGAAGCAGCTACTAAATGAATCTCAGCAGAAAATAGAGTCACAGAAAATGTTGCATGATGCCCAGTTATCAGAAGAACAAGGCCGCAACTTAGGGCTTCAAGCTCTCCTGGAGTCAGAGCAAGTTAGGATTCAGGAGATGAAGAGCACACTAGACAAGGAACGGGAGCTGTATGCCCAGTTGCAGAGCCGTGAAGATGGCGGGCAGCCCCCGCCAGCCTTGCCTTCTGAGGACCTACTTAAAGAGCTACAGAAACAACTGGAGGAAAAACACAGCCGCATAGTAGAACTGTTAAGTGAAACTGAAAAATATAAACTAGATTCCTTGCAAACAAGGCAACAGATGGAAAAGGACAGGCAGGTTCATCAGAAGACACTGCAGACAGAACAGGAGGCAAACACCCAGGGCCAAAAGAAAATGCAGGAGCTGCAGTCCAAAGTGGAAGAGCTGCAGCGCCAGCTCCAGGAGAAAAGGCAGCAGGTGTACAAGCTAGACCTGGAGGGAAAGCGACTGCAAGGGCTTATGCAGGAATTCCAAAAGCAGGAGCTGGAGCCGGAGGAGAAACCGGGAAGCAGAGGCCTTGTGGATCAGAATCTGAATGAGCCAGCCACCTGGAACTTCACTGATGATCGGACTAGAAATTGGGTTCTTCAACAGAAAATGGGAGAGGCCAAAGACAGGAACTTCACAAAGTTGATTGAAATAAACGGAGGAGAACTTGACCATAATCATGACTTGGAAATGATCAGACAGACACTCCAGCATGTGGCTTCAAAACTTCAGCATGTAGCCCAGAAAGCCTGCAGCAGGCTGCAGTTTGAAACAGCCGGTGATGATGCATTTATTTGGATTCAGGAGAATATTGATGGAATTATTTTGCAACTACAGAAACTAACTGGCCAGCCAGGTGATGAGCACAGCTTAGGACCCCCCAGTTCTTCCTGTGGCTCCTTAACTGAAAGCCTAATGAGACAAAATACTGAGTTAACAAGACTTATCAACCAACTAACAGAAGAAAAGAACACCTTACGGAGCATTGTCATAAAGCTGGAGGAGCTGAACAGGTGCTACTGGCACACAGGAGCCAGCAGAGACTGTTGTTCTAGGTTCTCATTTATTGATCCAGCTGACATCGAAGCCATCATTGCTTCAGAAAAGGAAGTCTGGAACAGAGAAAAGTTATCTCTTCAGAAAGCCTTGAAGAGGGCAGAAGCAAAAGTGTACAAGCTAAAAGCTGAACTACGAAACGATGCCTTGCTTCGGAATCTGGGCCCTGACACTGATCATGCTGCCCTGCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCTGAACTACGAAACGATGCC
59.94
AGCTGCTTCTGTTGTTCCAA
57.94
143
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GCTGAACTACGAAACGATGCC
59.94
AGTAGCTGCTTCTGTTGTTCCA
59.9
146
PCR template sequence:
GAAGAGGGCAGAAGCAAAAGTGTACAAGCTAAAAGCTGAACTACGAAACGATGCCTTGCTTCGGAATCTGGGCCCTGACACTGATCATGCTGCCCTGCAGGATCTGAAATTTTCACTTGAGGATCAGAAACGGAGAAATACACAGTTAAACCTCCTTTTGGAACAACAGAAGCAGCTACTAAATGAATCTCAGCAGAAAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-103-3p(1), mmu-miR-132-3p(1), mmu-miR-149-5p(1), mmu-miR-212-3p(1), mmu-miR-107-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TGCTGCCCTGCAGGATCTGAAATTTT
siRNA sets
ATTTCAGATCCTGCAGGGC, AATTTCAGATCCTGCAGGG, AAATTTCAGATCCTGCAGG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCAKAP9_1365_ORF_1
1.1509
0.23666206
0.999999943
2616
ORF sequence:
ATGTTGCATGATGCCCAGTTATCAGAAGAACAAGGCCGCAACTTAGGGCTTCAAGCTCTCCTGGAGTCAGAGCAAGTTAGGATTCAGGAGATGAAGAGCACACTAGACAAGGAACGGGAGCTGTATGCCCAGTTGCAGAGCCGTGAAGATGGCGGGCAGCCCCCGCCAGCCTTGCCTTCTGAGGACCTACTTAAAGAGCTACAGAAACAACTGGAGGAAAAACACAGCCGCATAGTAGAACTGTTAAGTGAAACTGAAAAATATAAACTAGATTCCTTGCAAACAAGGCAACAGATGGAAAAGGACAGGCAGGTTCATCAGAAGACACTGCAGACAGAACAGGAGGCAAACACCCAGGGCCAAAAGAAAATGCAGGAGCTGCAGTCCAAAGTGGAAGAGCTGCAGCGCCAGCTCCAGGAGAAAAGGCAGCAGGTGTACAAGCTAGACCTGGAGGGAAAGCGACTGCAAGGGCTTATGCAGGAATTCCAAAAGCAGGAGCTGGAGCCGGAGGAGAAACCGGGAAGCAGAGGCCTTGTGGATCAGAATCTGAATGAGCCAGCCACCTGGAACTTCACTGATGATCGGACTAGAAATTGGGTTCTTCAACAGAAAATGGGAGAGGCCAAAGACAGGAACTTCACAAAGTTGATTGAAATAAACGGAGGAGAACTTGACCATAATCATGACTTGGAAATGATCAGACAGACACTCCAGCATGTGGCTTCAAAACTTCAGCATGTAGCCCAGAAAGCCTGCAGCAGGCTGCAGTTTGAAACAGCCGGTGATGATGCATTTATTTGGATTCAGGAGAATATTGATGGAATTATTTTGCAACTACAGAAACTAACTGGCCAGCCAGGTGATGAGCACAGCTTAGGACCCCCCAGTTCTTCCTGTGGCTCCTTAACTGAAAGCCTAATGAGACAAAATACTGAGTTAACAAGACTTATCAACCAACTAACAGAAGAAAAGAACACCTTACGGAGCATTGTCATAAAGCTGGAGGAGCTGAACAGGTGCTACTGGCACACAGGAGCCAGCAGAGACTGTTGTTCTAGGTTCTCATTTATTGATCCAGCTGACATCGAAGCCATCATTGCTTCAGAAAAGGAAGTCTGGAACAGAGAAAAGTTATCTCTTCAGAAAGCCTTGAAGAGGGCAGAAGCAAAAGTGTACAAGCTAAAAGCTGAACTACGAAACGATGCCTTGCTTCGGAATCTGGGCCCTGACACTGATCATGCTGCCCTGCAGGATCTGAAATTTTCACTTGAGGATCAGAAACGGAGAAATACACAGTTAAACCTCCTTTTGGAACAACAGAAGCAGCTACTAAATGAATCTCAGCAGAAAATAGAGTCACAGAAAATGTTGCATGATGCCCAGTTATCAGAAGAACAAGGCCGCAACTTAGGGCTTCAAGCTCTCCTGGAGTCAGAGCAAGTTAGGATTCAGGAGATGAAGAGCACACTAGACAAGGAACGGGAGCTGTATGCCCAGTTGCAGAGCCGTGAAGATGGCGGGCAGCCCCCGCCAGCCTTGCCTTCTGAGGACCTACTTAAAGAGCTACAGAAACAACTGGAGGAAAAACACAGCCGCATAGTAGAACTGTTAAGTGAAACTGAAAAATATAAACTAGATTCCTTGCAAACAAGGCAACAGATGGAAAAGGACAGGCAGGTTCATCAGAAGACACTGCAGACAGAACAGGAGGCAAACACCCAGGGCCAAAAGAAAATGCAGGAGCTGCAGTCCAAAGTGGAAGAGCTGCAGCGCCAGCTCCAGGAGAAAAGGCAGCAGGTGTACAAGCTAGACCTGGAGGGAAAGCGACTGCAAGGGCTTATGCAGGAATTCCAAAAGCAGGAGCTGGAGCCGGAGGAGAAACCGGGAAGCAGAGGCCTTGTGGATCAGAATCTGAATGAGCCAGCCACCTGGAACTTCACTGATGATCGGACTAGAAATTGGGTTCTTCAACAGAAAATGGGAGAGGCCAAAGACAGGAACTTCACAAAGTTGATTGAAATAAACGGAGGAGAACTTGACCATAATCATGACTTGGAAATGATCAGACAGACACTCCAGCATGTGGCTTCAAAACTTCAGCATGTAGCCCAGAAAGCCTGCAGCAGGCTGCAGTTTGAAACAGCCGGTGATGATGCATTTATTTGGATTCAGGAGAATATTGATGGAATTATTTTGCAACTACAGAAACTAACTGGCCAGCCAGGTGATGAGCACAGCTTAGGACCCCCCAGTTCTTCCTGTGGCTCCTTAACTGAAAGCCTAATGAGACAAAATACTGAGTTAACAAGACTTATCAACCAACTAACAGAAGAAAAGAACACCTTACGGAGCATTGTCATAAAGCTGGAGGAGCTGAACAGGTGCTACTGGCACACAGGAGCCAGCAGAGACTGTTGTTCTAGGTTCTCATTTATTGATCCAGCTGACATCGAAGCCATCATTGCTTCAGAAAAGGAAGTCTGGAACAGAGAAAAGTTATCTCTTCAGAAAGCCTTGAAGAGGGCAGAAGCAAAAGTGTACAAGCTAAAAGCTGAACTACGAAACGATGCCTTGCTTCGGAATCTGGGCCCTGACACTGATCATGCTGCCCTGCAG
Peptide sequence:
MLHDAQLSEEQGRNLGLQALLESEQVRIQEMKSTLDKERELYAQLQSREDGGQPPPALPSEDLLKELQKQLEEKHSRIVELLSETEKYKLDSLQTRQQMEKDRQVHQKTLQTEQEANTQGQKKMQELQSKVEELQRQLQEKRQQVYKLDLEGKRLQGLMQEFQKQELEPEEKPGSRGLVDQNLNEPATWNFTDDRTRNWVLQQKMGEAKDRNFTKLIEINGGELDHNHDLEMIRQTLQHVASKLQHVAQKACSRLQFETAGDDAFIWIQENIDGIILQLQKLTGQPGDEHSLGPPSSSCGSLTESLMRQNTELTRLINQLTEEKNTLRSIVIKLEELNRCYWHTGASRDCCSRFSFIDPADIEAIIASEKEVWNREKLSLQKALKRAEAKVYKLKAELRNDALLRNLGPDTDHAALQDLKFSLEDQKRRNTQLNLLLEQQKQLLNESQQKIESQKMLHDAQLSEEQGRNLGLQALLESEQVRIQEMKSTLDKERELYAQLQSREDGGQPPPALPSEDLLKELQKQLEEKHSRIVELLSETEKYKLDSLQTRQQMEKDRQVHQKTLQTEQEANTQGQKKMQELQSKVEELQRQLQEKRQQVYKLDLEGKRLQGLMQEFQKQELEPEEKPGSRGLVDQNLNEPATWNFTDDRTRNWVLQQKMGEAKDRNFTKLIEINGGELDHNHDLEMIRQTLQHVASKLQHVAQKACSRLQFETAGDDAFIWIQENIDGIILQLQKLTGQPGDEHSLGPPSSSCGSLTESLMRQNTELTRLINQLTEEKNTLRSIVIKLEELNRCYWHTGASRDCCSRFSFIDPADIEAIIASEKEVWNREKLSLQKALKRAEAKVYKLKAELRNDALLRNLGPDTDHAALQ
IRESfinder result
IRESfinder score
0.483702092
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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