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mmu_circAkap10_922
Basic information of circular RNA
PanCircBase ID:
mmu_circAkap10_922
mm39_Chr. Position ID:
chr11|61768132|61794856|-
Gene name:
Akap10
Transcript:
ENSMUST00000102650.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
8
Exon/intron indexes:
14,13,12,11,10,9,8,7
Exon/intron offsets:
0,667,9393,12931,16073,19341,23003,26600
Genomic length:
26724
Exon/intron sizes in nt:
96,53,83,11,01,74,14,50,00,000
Flanking exon/introns:
chr11:61763847-61768132|chr11:61794856-61795600
Splice length:
922
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GCACTTTAGTGAGTTTCTGCGAAGTCACCATTTCTGTAAATACCAGATTGAAGTGCTGACCAGTGGGACTGTTTACCTGGCTGATATCCTCTTCTGTGAGTCAGCCCTCTTTTATTTTTCTGAGTACATGGAAAAAGAAGATGCAGTGAATATCTTACAATTCTGGTTAGCAGCGGATAATTTCCAGTCTCAGCTTGCTGCCAAAAAGGGCCAGTATGATGGACAGGAGGCCCAGAATGATGCCATGATTTTATATGACAAGTACTTTTCCCTCCAAGCCACACACCCCCTTGGATTTGATGATGTTGTACGATTAGAAATTGAATCTAATATCTGCAGGGAAGGTGGACCACTTCCTAATTGTTTCACAACTCCATTACGTCAGGCCTGGACAACCATGGAGAAGGTCTTTTTGCCTGGTTTTCTGTCCAGCAATCTTTATTACAAATATTTGAATGATCTCATCCATTCAGTTCGAGGAGATGAATTTCTTGGAGGGAATGTTTCCCTGGCTGCTCACGGCTCTGTCTGCCTTCCTGAGGAGTCTCACTCAGGTGGTTCCGATGGCTCCACTGCTCAGTCTAGTGTGAAAAAAGCCAGTATTAAAATTCTGAAAAATTTTGATGAAGCAATAATTGTGGATGCTGCAAGTCTGGACCCAGAATCTTTATATCAACGGACATATGCAGGGAAGATGTCCTTTGGGAGAGTTAGTGATTTGGGGCAGTTCATCCGAGAGTCTGAGCCTGAACCTGATGTGAAGAAATCAAAAGGATTCATGTTCTCACAAGCTATGAAGAAGTGGGTGCAAGGAAATACTGACGAGGCCCAAGAAGAGCTAGCTTGGAAGATTGCAAAAATGATAGTGAGTGATGTTATGCAGCAGGCACACCATGATCAACCACTAGAGAAGTCTACAAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CGAGGCCCAAGAAGAGCTAG
59.9
AAACAGTCCCACTGGTCAGC
60.18
174
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CGAGGCCCAAGAAGAGCTAG
59.9
CCCACTGGTCAGCACTTCAA
60.18
167
PCR template sequence:
CGAGGCCCAAGAAGAGCTAGCTTGGAAGATTGCAAAAATGATAGTGAGTGATGTTATGCAGCAGGCACACCATGATCAACCACTAGAGAAGTCTACAAAGGCACTTTAGTGAGTTTCTGCGAAGTCACCATTTCTGTAAATACCAGATTGAAGTGCTGACCAGTGGGACTGTTTACCTGGCTGATATCCTCTTCTGTGAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
12
Interacting miRNAs (#binding sites)
mmu-miR-30e-5p(1), mmu-miR-30a-5p(1), mmu-miR-30c-5p(1), mmu-miR-328-3p(1), mmu-miR-30d-5p(1), mmu-miR-214-3p(1), mmu-miR-24-3p(1), mmu-miR-30b-5p(1), mmu-miR-212-3p(1), mmu-miR-129-5p(1), mmu-miR-207(1), mmu-miR-484(1)
siRNAs for circular RNA silencing
Junction sequence
GAAGTCTACAAAGGCACTTTAGTGAG
siRNA sets
ACTAAAGTGCCTTTGTAGA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCAKAP10_922_ORF_1
1.0387
0.116794458
0.949061713
804
ORF sequence:
ATGGAAAAAGAAGATGCAGTGAATATCTTACAATTCTGGTTAGCAGCGGATAATTTCCAGTCTCAGCTTGCTGCCAAAAAGGGCCAGTATGATGGACAGGAGGCCCAGAATGATGCCATGATTTTATATGACAAGTACTTTTCCCTCCAAGCCACACACCCCCTTGGATTTGATGATGTTGTACGATTAGAAATTGAATCTAATATCTGCAGGGAAGGTGGACCACTTCCTAATTGTTTCACAACTCCATTACGTCAGGCCTGGACAACCATGGAGAAGGTCTTTTTGCCTGGTTTTCTGTCCAGCAATCTTTATTACAAATATTTGAATGATCTCATCCATTCAGTTCGAGGAGATGAATTTCTTGGAGGGAATGTTTCCCTGGCTGCTCACGGCTCTGTCTGCCTTCCTGAGGAGTCTCACTCAGGTGGTTCCGATGGCTCCACTGCTCAGTCTAGTGTGAAAAAAGCCAGTATTAAAATTCTGAAAAATTTTGATGAAGCAATAATTGTGGATGCTGCAAGTCTGGACCCAGAATCTTTATATCAACGGACATATGCAGGGAAGATGTCCTTTGGGAGAGTTAGTGATTTGGGGCAGTTCATCCGAGAGTCTGAGCCTGAACCTGATGTGAAGAAATCAAAAGGATTCATGTTCTCACAAGCTATGAAGAAGTGGGTGCAAGGAAATACTGACGAGGCCCAAGAAGAGCTAGCTTGGAAGATTGCAAAAATGATAGTGAGTGATGTTATGCAGCAGGCACACCATGATCAACCACTAGAGAAGTCTACAAAGGCACTTTAG
Peptide sequence:
MEKEDAVNILQFWLAADNFQSQLAAKKGQYDGQEAQNDAMILYDKYFSLQATHPLGFDDVVRLEIESNICREGGPLPNCFTTPLRQAWTTMEKVFLPGFLSSNLYYKYLNDLIHSVRGDEFLGGNVSLAAHGSVCLPEESHSGGSDGSTAQSSVKKASIKILKNFDEAIIVDAASLDPESLYQRTYAGKMSFGRVSDLGQFIRESEPEPDVKKSKGFMFSQAMKKWVQGNTDEAQEELAWKIAKMIVSDVMQQAHHDQPLEKSTKAL
IRESfinder result
IRESfinder score
0.662320658
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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