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mmu_circAkap10_282
Basic information of circular RNA
PanCircBase ID:
mmu_circAkap10_282
mm39_Chr. Position ID:
chr11|61787473|61791272|-
Gene name:
Akap10
Transcript:
ENSMUST00000102650.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
9,8
Exon/intron offsets:
0,3662
Genomic length:
3799
Exon/intron sizes in nt:
1,45,137
Flanking exon/introns:
chr11:61784379-61787473|chr11:61791272-61794732
Splice length:
282
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
TACATGGAAAAAGAAGATGCAGTGAATATCTTACAATTCTGGTTAGCAGCGGATAATTTCCAGTCTCAGCTTGCTGCCAAAAAGGGCCAGTATGATGGACAGGAGGCCCAGAATGATGCCATGATTTTATATGACAAGTACTTTTCCCTCCAAGCCACACACCCCCTTGGATTTGATGATGTTGTACGATTAGAAATTGAATCTAATATCTGCAGGGAAGGTGGACCACTTCCTAATTGTTTCACAACTCCATTACGTCAGGCCTGGACAACCATGGAGAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CAGGGAAGGTGGACCACTTC
59.96
TTTGGCAGCAAGCTGAGACT
60.18
151
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CAGGGAAGGTGGACCACTTC
59.96
CCTTTTTGGCAGCAAGCTGA
59.61
155
PCR template sequence:
TGTACGATTAGAAATTGAATCTAATATCTGCAGGGAAGGTGGACCACTTCCTAATTGTTTCACAACTCCATTACGTCAGGCCTGGACAACCATGGAGAAGTACATGGAAAAAGAAGATGCAGTGAATATCTTACAATTCTGGTTAGCAGCGGATAATTTCCAGTCTCAGCTTGCTGCCAAAAAGGGCCAGTATGATGGAC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-328-3p(1), mmu-miR-214-3p(1), mmu-miR-207(1)
siRNAs for circular RNA silencing
Junction sequence
AACCATGGAGAAGTACATGGAAAAAG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCAKAP10_282_ORF_1
0.8879
0.161730311
0.75109677
561
ORF sequence:
ATGGAAAAAGAAGATGCAGTGAATATCTTACAATTCTGGTTAGCAGCGGATAATTTCCAGTCTCAGCTTGCTGCCAAAAAGGGCCAGTATGATGGACAGGAGGCCCAGAATGATGCCATGATTTTATATGACAAGTACTTTTCCCTCCAAGCCACACACCCCCTTGGATTTGATGATGTTGTACGATTAGAAATTGAATCTAATATCTGCAGGGAAGGTGGACCACTTCCTAATTGTTTCACAACTCCATTACGTCAGGCCTGGACAACCATGGAGAAGTACATGGAAAAAGAAGATGCAGTGAATATCTTACAATTCTGGTTAGCAGCGGATAATTTCCAGTCTCAGCTTGCTGCCAAAAAGGGCCAGTATGATGGACAGGAGGCCCAGAATGATGCCATGATTTTATATGACAAGTACTTTTCCCTCCAAGCCACACACCCCCTTGGATTTGATGATGTTGTACGATTAGAAATTGAATCTAATATCTGCAGGGAAGGTGGACCACTTCCTAATTGTTTCACAACTCCATTACGTCAGGCCTGGACAACCATGGAGAAG
Peptide sequence:
MEKEDAVNILQFWLAADNFQSQLAAKKGQYDGQEAQNDAMILYDKYFSLQATHPLGFDDVVRLEIESNICREGGPLPNCFTTPLRQAWTTMEKYMEKEDAVNILQFWLAADNFQSQLAAKKGQYDGQEAQNDAMILYDKYFSLQATHPLGFDDVVRLEIESNICREGGPLPNCFTTPLRQAWTTMEK
IRESfinder result
IRESfinder score
0.493682073
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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