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mmu_circAk2_196
Basic information of circular RNA
PanCircBase ID:
mmu_circAk2_196
mm39_Chr. Position ID:
chr4|128901826|128902022|+
Gene name:
Ak2
Transcript:
ENSMUST00000102604.11
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
1
Exon/intron indexes:
6
Exon/intron offsets:
0
Genomic length:
196
Exon/intron sizes in nt:
196
Flanking exon/introns:
chr4:128901578-128901826|chr4:128902022-128904355
Splice length:
196
Other database IDs:
circAtlas: mmu-Ak2_0001
CIRCpedia: HSA_CIRCpedia_31474
Human Ortholog IDs:
Mature spliced Sequence:
ATCACTGGGGAGCCCCTGATCCGCAGGTCAGATGACAACGAGAAAGCCTTGAAGACCCGCCTGGAGGCCTACCACACTCAGACCACTCCGCTCGTGGAGTACTACAGGAAACGCGGCATTCACTGCGCCATCGATGCGTCCCAGACCCCTGACATCGTGTTTGCGAGCATCCTGGCAGCCTTCTCCAAAGCCACAT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CATTCACTGCGCCATCGATG
60.04
GTGGTCTGAGTGTGGTAGGC
60.04
166
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CTGACATCGTGTTTGCGAGC
60.18
GTGGTCTGAGTGTGGTAGGC
60.04
134
PCR template sequence:
GTACTACAGGAAACGCGGCATTCACTGCGCCATCGATGCGTCCCAGACCCCTGACATCGTGTTTGCGAGCATCCTGGCAGCCTTCTCCAAAGCCACATATCACTGGGGAGCCCCTGATCCGCAGGTCAGATGACAACGAGAAAGCCTTGAAGACCCGCCTGGAGGCCTACCACACTCAGACCACTCCGCTCGTGGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
NA
Interacting miRNAs (#binding sites)
NA
siRNAs for circular RNA silencing
Junction sequence
TCCAAAGCCACATATCACTGGGGAGC
siRNA sets
AGTGATATGTGGCTTTGGA, CAGTGATATGTGGCTTTGG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCAK2_196_ORF_1
0.9846
0.45750122
0.577138916
258
ORF sequence:
ATGCGTCCCAGACCCCTGACATCGTGTTTGCGAGCATCCTGGCAGCCTTCTCCAAAGCCACATATCACTGGGGAGCCCCTGATCCGCAGGTCAGATGACAACGAGAAAGCCTTGAAGACCCGCCTGGAGGCCTACCACACTCAGACCACTCCGCTCGTGGAGTACTACAGGAAACGCGGCATTCACTGCGCCATCGATGCGTCCCAGACCCCTGACATCGTGTTTGCGAGCATCCTGGCAGCCTTCTCCAAAGCCACA
Peptide sequence:
MRPRPLTSCLRASWQPSPKPHITGEPLIRRSDDNEKALKTRLEAYHTQTTPLVEYYRKRGIHCAIDASQTPDIVFASILAAFSKAT
IRESfinder result
IRESfinder score
0.387802435
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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