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mmu_circAdgrv1_2491
Basic information of circular RNA
PanCircBase ID:
mmu_circAdgrv1_2491
mm39_Chr. Position ID:
chr13|81666071|81677177|-
Gene name:
Adgrv1
Transcript:
ENSMUST00000095585.11
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
34,33,32,31,30,29,28
Exon/intron offsets:
0,2550,4152,5583,6606,7361,10496
Genomic length:
11106
Exon/intron sizes in nt:
210,812,182,245,216,216,610
Flanking exon/introns:
chr13:81659744-81666071|chr13:81677177-81679685
Splice length:
2491
Other database IDs:
circBase: mmu_circ_0004825, CIRCpedia: MMU_CIRCpedia_30859, circAtlas: mmu-Gpr98_0006
circAtlas: hsa-ADGRV1_0019
Human Ortholog IDs:
Mature spliced Sequence:
GTTACACGGACTCGGGGAGCCCTGTCTGCAGTGACTTTGCAATGGAAGGTAGACTCGGACCTGGATGGGGATCTCGCCATTACATCTGGCAACATCACATTTGAGACTGGGCAGAGGATTGCTTCCATCACTGTGGAGATACTGTCAGATGAAGAGCCAGAGCTAGACAAGGCACTCACCGTGTCGATCCTCAACGTGTCCAGTGGCTCCTTGGGAGTTCTTACAAATGCCACATTGACAATTTTGGCTAGTGATGATCCTTATGGGGTCTTTATTTTTCCTAACAAAACTAGACCTTTGAGTGTTGAAGAAGCAACCCAGAATGTCACATTATCGATAATAAGGTTGAAAGGCCTCATGGGAGAAGTTGCAGTCTCATATGCAACCATAGATGATATGGAAAAGCCACCGTATTTCCCACCTAATTTAGCTAGAGCAACTCAAGGAGGAGATTACATATCAGCATCTGGATTGGCTCTTTTCAGAGCTAATCAGACTGAGGCAACAATCACTATTTCAATCCTAGATGATGCTGAACCAGAACGCTCAGAATCTGTGTTCATTGAACTTTTCAATTCCTCTTTAGTAGACAAAGTACAGAATCGCCCAATCCCACATTCTCCACGCCTTGGGCCTAAGGTGGAGACTGTGGCCCATCTCGTTATTGTTGCCAATGACGATGCATTTGGAACTGTGCAGCTGTCTGCAACATCTGTTCATGTAGCAGAAAATCATGTTGGACCCATTATCAATGTGACTCGAACTGGAGGAACATTTGCAGATGTTTCTGTTAAGTTTAAAGCTGTGCCAATAACTGCAGCAGCGGGTGAGGACTATAGTATAGCATCTTCAGACGTGGTCTTGCTGGAAGGGGAAACCACTAAAGCTGTGCCAATATATATCATTAACGACATCTACCCTGAGCTGGAAGAAACCTTTCTTGTGCAGCTACTAAACGAAACAACAGGTGGAGCCACACTGGGGCCTCTGAGAGAGGCAGTCATTACCATAGAGGCGTCTGATGACCCCTACGGACTGTTTGGTTTTCAGAATACTAAATTTATTGTAGAGGAACCTGAGTTTAACTCAGTGAGGGTAAACGTGCCAATAATTCGAAATTCTGGGACACTCGGCAATGTTACTGTTCAATGGGTTGCCATCATTAATGGACAGTTTGCTACTGGCGACCTGCGAGTTGTCTCAGGTAATGTGACCTTTGCCCCTGGGGAAACCATTCAAACCTTGTTGTTAGAGGTCCTGGCTGACGACGTTCCGGAGATTGAAGAGGTTGTCCAGGTGCAACTAGCTGCTGCCTCTGGCGGAGGTACAATTGGGTTAGATCGAGTGGCAAATATTGTTATTCCTGCCAATGATAACCCTTACGGTTCAGTAGCCTTTGTTCAGTCCGTTTTTCGTGTCCAAGAGCCTCTAGAGAGAAGTTCCTATGCTAACATAACTGTCAGGAGAAGCGGAGGACACTTTGGTCGCCTGCTGTTGTGCTATGGTACTTCTGATATTGATGTAGTGGCTCGTGCAGTTGAGGAAGGTGAAGATGTGTTATCCTACTATGAATCACCGACTCAAGGGGTGCCCGACCCACTCTGGAGAACTTGGGTGAACGTGTCTGCAGTGGAGGAGACACAGTATACCTGTGCCACTTTGTGTCTCAAAGAACGTGCCTGCTCAGCGTTTTCAGTTGTCAGTGGTGCCGAGGGCCCTCGGTGCTTCTGGATGACGTCGTGGGTCAGCGGAACTGTGAACAGCTCTGACTTCCAAACCTACAAGAAGAACATGACTAGGGTGGCCTCTCTTTTCAGTGGCCAGGCAGTTGCTGGTAGTGACTACGAGCCTGTGACAAGACAGTGGGCCGTGATACTGGAAGGTGATGAGTTTGCAAATCTCACTGTTTCTGTACTTCCTGACGATGCTCCCGAGATGGATGAAAGTTTCCTAATTTCTCTCCTTGAAGTTCACCTTATGAACATCTCAGACAGTTTTAAAAACCAGCCAACCATAGGACATCCGAATACTTCCGCTGTGGTCATAGGACTGAATGGCGATGCCTTTGGAGTATTCATTATCTACAGTGTTAGTCCCAATACCTCGGAAGATGGCTTATGTGTGGAAGTGCAGGAACAGCCACAAACTTCTGTGGAACTGGTTATCTACAGGACAGGAGGCAGCCTGGGGCAGGTCATGGTCGAATGGCGCGTTGTTGGTGGAACGGCTACTGAAGGTTTAGATTTTATGGGTGCTGGAGACATTCTTACTTTTGCAGAAGGTGAAACCAAAAAGATGGCCATTTTAACCATTTTGGATGATTCTGAGCCAGAGGACAATGAAAGCATCCTTGTCCGTCTGGTGGCCACAGAGGGCGGAAGCAGAATCCTGCCCAGCTCAGACACCGTGACAGTCAACATCTTGGCAAACGACAATGTGGCAGGAATTGTCAGCTTTCAGACAGCTTCCAGATCTGTCATAGGCCACGAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCTCAGACACCGTGACAGTC
60.66
CCCATCCAGGTCCGAGTCTA
60.11
165
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CAGCTCAGACACCGTGACAG
60.67
CCCATCCAGGTCCGAGTCTA
60.11
167
PCR template sequence:
CCCAGCTCAGACACCGTGACAGTCAACATCTTGGCAAACGACAATGTGGCAGGAATTGTCAGCTTTCAGACAGCTTCCAGATCTGTCATAGGCCACGAAGGTTACACGGACTCGGGGAGCCCTGTCTGCAGTGACTTTGCAATGGAAGGTAGACTCGGACCTGGATGGGGATCTCGCCATTACATCTGGCAACATCACAT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
23
Interacting miRNAs (#binding sites)
mmu-miR-29b-2-5p(3), mmu-miR-23b-3p(2), mmu-miR-23a-3p(2), mmu-miR-149-5p(2), mmu-miR-140-5p(1), mmu-miR-200a-3p(1), mmu-miR-141-3p(1), mmu-miR-199a-5p(1), mmu-miR-221-3p(1), mmu-miR-709(1), mmu-miR-185-5p(1), mmu-miR-214-3p(1), mmu-miR-24-3p(1), mmu-miR-433-5p(1), mmu-miR-125a-5p(1), mmu-miR-182-5p(1), mmu-miR-705(1), mmu-miR-377-3p(1), mmu-miR-207(1), mmu-miR-539-5p(1), mmu-miR-484(1), mmu-miR-452-3p(1), mmu-miR-1904(1)
siRNAs for circular RNA silencing
Junction sequence
ATAGGCCACGAAGGTTACACGGACTC
siRNA sets
AGTCCGTGTAACCTTCGTG, CCGTGTAACCTTCGTGGCC, GAGTCCGTGTAACCTTCGT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCADGRV1_2491_ORF_1
1.0679
0.149058884
0.999998463
2238
ORF sequence:
ATGGGAGAAGTTGCAGTCTCATATGCAACCATAGATGATATGGAAAAGCCACCGTATTTCCCACCTAATTTAGCTAGAGCAACTCAAGGAGGAGATTACATATCAGCATCTGGATTGGCTCTTTTCAGAGCTAATCAGACTGAGGCAACAATCACTATTTCAATCCTAGATGATGCTGAACCAGAACGCTCAGAATCTGTGTTCATTGAACTTTTCAATTCCTCTTTAGTAGACAAAGTACAGAATCGCCCAATCCCACATTCTCCACGCCTTGGGCCTAAGGTGGAGACTGTGGCCCATCTCGTTATTGTTGCCAATGACGATGCATTTGGAACTGTGCAGCTGTCTGCAACATCTGTTCATGTAGCAGAAAATCATGTTGGACCCATTATCAATGTGACTCGAACTGGAGGAACATTTGCAGATGTTTCTGTTAAGTTTAAAGCTGTGCCAATAACTGCAGCAGCGGGTGAGGACTATAGTATAGCATCTTCAGACGTGGTCTTGCTGGAAGGGGAAACCACTAAAGCTGTGCCAATATATATCATTAACGACATCTACCCTGAGCTGGAAGAAACCTTTCTTGTGCAGCTACTAAACGAAACAACAGGTGGAGCCACACTGGGGCCTCTGAGAGAGGCAGTCATTACCATAGAGGCGTCTGATGACCCCTACGGACTGTTTGGTTTTCAGAATACTAAATTTATTGTAGAGGAACCTGAGTTTAACTCAGTGAGGGTAAACGTGCCAATAATTCGAAATTCTGGGACACTCGGCAATGTTACTGTTCAATGGGTTGCCATCATTAATGGACAGTTTGCTACTGGCGACCTGCGAGTTGTCTCAGGTAATGTGACCTTTGCCCCTGGGGAAACCATTCAAACCTTGTTGTTAGAGGTCCTGGCTGACGACGTTCCGGAGATTGAAGAGGTTGTCCAGGTGCAACTAGCTGCTGCCTCTGGCGGAGGTACAATTGGGTTAGATCGAGTGGCAAATATTGTTATTCCTGCCAATGATAACCCTTACGGTTCAGTAGCCTTTGTTCAGTCCGTTTTTCGTGTCCAAGAGCCTCTAGAGAGAAGTTCCTATGCTAACATAACTGTCAGGAGAAGCGGAGGACACTTTGGTCGCCTGCTGTTGTGCTATGGTACTTCTGATATTGATGTAGTGGCTCGTGCAGTTGAGGAAGGTGAAGATGTGTTATCCTACTATGAATCACCGACTCAAGGGGTGCCCGACCCACTCTGGAGAACTTGGGTGAACGTGTCTGCAGTGGAGGAGACACAGTATACCTGTGCCACTTTGTGTCTCAAAGAACGTGCCTGCTCAGCGTTTTCAGTTGTCAGTGGTGCCGAGGGCCCTCGGTGCTTCTGGATGACGTCGTGGGTCAGCGGAACTGTGAACAGCTCTGACTTCCAAACCTACAAGAAGAACATGACTAGGGTGGCCTCTCTTTTCAGTGGCCAGGCAGTTGCTGGTAGTGACTACGAGCCTGTGACAAGACAGTGGGCCGTGATACTGGAAGGTGATGAGTTTGCAAATCTCACTGTTTCTGTACTTCCTGACGATGCTCCCGAGATGGATGAAAGTTTCCTAATTTCTCTCCTTGAAGTTCACCTTATGAACATCTCAGACAGTTTTAAAAACCAGCCAACCATAGGACATCCGAATACTTCCGCTGTGGTCATAGGACTGAATGGCGATGCCTTTGGAGTATTCATTATCTACAGTGTTAGTCCCAATACCTCGGAAGATGGCTTATGTGTGGAAGTGCAGGAACAGCCACAAACTTCTGTGGAACTGGTTATCTACAGGACAGGAGGCAGCCTGGGGCAGGTCATGGTCGAATGGCGCGTTGTTGGTGGAACGGCTACTGAAGGTTTAGATTTTATGGGTGCTGGAGACATTCTTACTTTTGCAGAAGGTGAAACCAAAAAGATGGCCATTTTAACCATTTTGGATGATTCTGAGCCAGAGGACAATGAAAGCATCCTTGTCCGTCTGGTGGCCACAGAGGGCGGAAGCAGAATCCTGCCCAGCTCAGACACCGTGACAGTCAACATCTTGGCAAACGACAATGTGGCAGGAATTGTCAGCTTTCAGACAGCTTCCAGATCTGTCATAGGCCACGAAGGTTACACGGACTCGGGGAGCCCTGTCTGCAGTGACTTTGCAATGGAAGGTAGACTCGGACCTGGATGGGGATCTCGCCATTACATCTGGCAACATCACATTTGA
Peptide sequence:
MGEVAVSYATIDDMEKPPYFPPNLARATQGGDYISASGLALFRANQTEATITISILDDAEPERSESVFIELFNSSLVDKVQNRPIPHSPRLGPKVETVAHLVIVANDDAFGTVQLSATSVHVAENHVGPIINVTRTGGTFADVSVKFKAVPITAAAGEDYSIASSDVVLLEGETTKAVPIYIINDIYPELEETFLVQLLNETTGGATLGPLREAVITIEASDDPYGLFGFQNTKFIVEEPEFNSVRVNVPIIRNSGTLGNVTVQWVAIINGQFATGDLRVVSGNVTFAPGETIQTLLLEVLADDVPEIEEVVQVQLAAASGGGTIGLDRVANIVIPANDNPYGSVAFVQSVFRVQEPLERSSYANITVRRSGGHFGRLLLCYGTSDIDVVARAVEEGEDVLSYYESPTQGVPDPLWRTWVNVSAVEETQYTCATLCLKERACSAFSVVSGAEGPRCFWMTSWVSGTVNSSDFQTYKKNMTRVASLFSGQAVAGSDYEPVTRQWAVILEGDEFANLTVSVLPDDAPEMDESFLISLLEVHLMNISDSFKNQPTIGHPNTSAVVIGLNGDAFGVFIIYSVSPNTSEDGLCVEVQEQPQTSVELVIYRTGGSLGQVMVEWRVVGGTATEGLDFMGAGDILTFAEGETKKMAILTILDDSEPEDNESILVRLVATEGGSRILPSSDTVTVNILANDNVAGIVSFQTASRSVIGHEGYTDSGSPVCSDFAMEGRLGPGWGSRHYIWQHHI
IRESfinder result
IRESfinder score
0.591118136
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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