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mmu_circAdgrl3_214
Basic information of circular RNA
PanCircBase ID:
mmu_circAdgrl3_214
mm39_Chr. Position ID:
chr5|81535621|81535835|+
Gene name:
Adgrl3
Transcript:
ENSMUST00000149280.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
1
Exon/intron indexes:
6
Exon/intron offsets:
0
Genomic length:
214
Exon/intron sizes in nt:
214
Flanking exon/introns:
chr5:81477807-81535621|chr5:81535835-81613041
Splice length:
214
Other database IDs:
CIRCpedia: MMU_CIRCpedia_37278, circAtlas: mmu-Lphn3_0012
circBase: hsa_circ_0126741, circAtlas: hsa-ADGRL3_0012, CIRCpedia: HSA_CIRCpedia_123934
Human Ortholog IDs:
Mature spliced Sequence:
CTTTCAGCCGCGCCCCAATTCCCATGGCAGTGGTCCGCAGAGAGCTCTCCTGTGAGAGCTACCCCATTGAGCTCCGTTGTCCGGGCACAGACGTCATCATGATCGAGAGCGCCAACTACGGGAGGACCGATGACAAGATCTGTGACTCGGACCCTGCTCAGATGGAGAACATTCGGTGTTATCTGCCAGATGCCTATAAGATTATGTCTCAAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGATCTGTGACTCGGACCCT
59.67
CAACGGAGCTCAATGGGGTA
59.75
158
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGATCTGTGACTCGGACCCT
59.67
TGGGGTAGCTCTCACAGGAG
60.32
145
PCR template sequence:
ACTACGGGAGGACCGATGACAAGATCTGTGACTCGGACCCTGCTCAGATGGAGAACATTCGGTGTTATCTGCCAGATGCCTATAAGATTATGTCTCAAAGCTTTCAGCCGCGCCCCAATTCCCATGGCAGTGGTCCGCAGAGAGCTCTCCTGTGAGAGCTACCCCATTGAGCTCCGTTGTCCGGGCACAGACGTCATCAT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-433-3p(1), mmu-miR-680(1)
siRNAs for circular RNA silencing
Junction sequence
TTATGTCTCAAAGCTTTCAGCCGCGC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCADGRL3_214_ORF_1
0.9414
0.287101792
0.453785992
297
ORF sequence:
ATGACAAGATCTGTGACTCGGACCCTGCTCAGATGGAGAACATTCGGTGTTATCTGCCAGATGCCTATAAGATTATGTCTCAAAGCTTTCAGCCGCGCCCCAATTCCCATGGCAGTGGTCCGCAGAGAGCTCTCCTGTGAGAGCTACCCCATTGAGCTCCGTTGTCCGGGCACAGACGTCATCATGATCGAGAGCGCCAACTACGGGAGGACCGATGACAAGATCTGTGACTCGGACCCTGCTCAGATGGAGAACATTCGGTGTTATCTGCCAGATGCCTATAAGATTATGTCTCAA
Peptide sequence:
MTRSVTRTLLRWRTFGVICQMPIRLCLKAFSRAPIPMAVVRRELSCESYPIELRCPGTDVIMIESANYGRTDDKICDSDPAQMENIRCYLPDAYKIMSQ
IRESfinder result
IRESfinder score
0.141978704
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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