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mmu_circAdgrl3_1714
Basic information of circular RNA
PanCircBase ID:
mmu_circAdgrl3_1714
mm39_Chr. Position ID:
chr5|81836355|81922278|+
Gene name:
Adgrl3
Transcript:
ENSMUST00000117253.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
12
Exon/intron indexes:
14,15,16,17,18,19,20,21,22,23,24,25
Exon/intron offsets:
0,1056,4984,5942,35553,38160,50518,52546,72118,77629,83075,85884
Genomic length:
85923
Exon/intron sizes in nt:
184,123,209,177,210,221,67,92,169,126,97,39
Flanking exon/introns:
chr5:81811271-81836355|chr5:81922278-81935282
Splice length:
1714
Other database IDs:
circAtlas: hsa-ADGRL3_0039
Human Ortholog IDs:
Mature spliced Sequence:
GCGATGGTGGAGACAGTTAACAACCTCCTTCAGCCACAAGCTTTGAATGCCTGGAGAGACCTGACGACAAGTGATCAACTGCGTGCGGCCACCATGTTGCTTGACACTGTGGAGGAGAGCGCTTTCGTGTTAGCTGATAACCTTTTGAAGACCGACATTGTCAGGGAGAATACAGACAATATTCAGTTAGAAGTTGCAAGACTGAGCACGGAAGGAAACCTAGAAGATCTAAAATTCCCAGAGAACATGGGCCACGGAAGTACCATCCAGCTCTCAGCAAACACATTAAAGCAAAATGGCCGGAATGGAGAGATTAGAGTGGCTTTTGTCCTGTATAATAACCTGGGTCCTTATTTGTCTACGGAGAATGCCAGTATGAAGTTGGGCACAGAAGCTATGTCCACAAATCACTCGGTTATCGTCAATTCCCCTGTTATTACAGCAGCAATAAATAAGGAATTCAGTAATAAAGTGTATTTGGCTGATCCTGTGGTATTTACTGTTAAACACATCAAGCAGTCAGAAGAAAATTTCAACCCTAACTGTTCATTTTGGAGCTATTCCAAGCGCACAATGACAGGTTATTGGTCAACACAAGGCTGTCGACTCCTGACAACCAACAAGACACATACTACATGCTCCTGTAACCACCTCACCAACTTTGCAGTATTGATGGCACATGTGGAAGTTAAGCACAGCGATGCAGTCCACGATCTTCTTCTGGATGTGATCACGTGGGTTGGCATCTTGCTGTCCCTTGTCTGTCTCCTGATTTGCATCTTCACATTCTGCTTCTTCCGGGGACTCCAGAGTGACCGCAACACCATTCACAAGAACCTCTGCATCAGCCTGTTCGTGGCAGAACTGCTCTTCCTGATTGGAATCAACAGAACCGACCAACCAATTGCCTGTGCAGTGTTCGCGGCTCTTTTGCATTTCTTCTTCTTGGCGGCCTTCACCTGGATGTTTCTAGAAGGGGTGCAGCTGTATATCATGCTGGTGGAGGTCTTTGAGAGCGAGCATTCCCGTAGGAAGTACTTTTATCTGGTCGGCTATGGGATGCCCGCGCTCATCGTGGCCGTTTCTGCGGCCGTCGACTACAGGAGCTACGGAACAGACAAAGTATGTTGGCTTCGCCTTGACACCTACTTCATTTGGAGTTTTATAGGACCGGCGACCTTGATAATTATGCTGAATGTCATCTTCCTTGGGATTGCTTTATACAAAATGTTTCACCATACTGCCATACTGAAACCTGAATCAGGCTGTCTTGACAATATCAAGTCATGGGTTATAGGTGCAATAGCTCTGCTCTGCCTGTTAGGATTGACCTGGGCCTTTGGACTCATGTATATTAATGAAAGCACAGTCATCATGGCGTATCTCTTCACCATTTTCAATTCTCTACAGGGAATGTTTATATTCATTTTCCACTGTGTTCTACAGAAGAAGGTACGGAAAGAGTATGGGAAATGCCTCCGCACGCATTGCTGTAGTGGGAAAAGCACGGAGAGTTCGATTGGCTCAGGGAAAACATCTGGTTCTCGAACTCCAGGACGGTACTCCACAGGCTCGCAGAGCCGGATTCGGAGAATGTGGAATGACACTGTCCGAAAGCAGTCAGAGTCGTCCTTCATCACCGGAGACATAAACAGCTCAGCATCGCTCAACAGAGGATATCTTCCCTGCATGCAGGCCTGTGTAACCTATGTAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GTCGTCCTTCATCACCGGAG
60.18
AGGCATTCAAAGCTTGTGGC
59.68
141
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GAGTCGTCCTTCATCACCGG
60.18
AGGCATTCAAAGCTTGTGGC
59.68
143
PCR template sequence:
AAGCAGTCAGAGTCGTCCTTCATCACCGGAGACATAAACAGCTCAGCATCGCTCAACAGAGGATATCTTCCCTGCATGCAGGCCTGTGTAACCTATGTAGGCGATGGTGGAGACAGTTAACAACCTCCTTCAGCCACAAGCTTTGAATGCCTGGAGAGACCTGACGACAAGTGATCAACTGCGTGCGGCCACCATGTTGC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
21
Interacting miRNAs (#binding sites)
mmu-miR-200c-3p(2), mmu-miR-670-5p(2), mmu-miR-137-3p(1), mmu-miR-200a-3p(1), mmu-miR-141-3p(1), mmu-miR-1a-3p(1), mmu-miR-200b-3p(1), mmu-miR-181a-5p(1), mmu-miR-31-5p(1), mmu-miR-92a-3p(1), mmu-miR-324-5p(1), mmu-miR-25-3p(1), mmu-miR-363-3p(1), mmu-miR-125a-5p(1), mmu-miR-494-3p(1), mmu-miR-676-3p(1), mmu-miR-218-5p(1), mmu-miR-340-5p(1), mmu-miR-1198-5p(1), mmu-miR-3470b(1), mmu-miR-181d-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GTAACCTATGTAGGCGATGGTGGAGA
siRNA sets
CCACCATCGCCTACATAGG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCADGRL3_1714_ORF_1
0.9716
0.158409758
0.999933799
1731
ORF sequence:
ATGGTGGAGACAGTTAACAACCTCCTTCAGCCACAAGCTTTGAATGCCTGGAGAGACCTGACGACAAGTGATCAACTGCGTGCGGCCACCATGTTGCTTGACACTGTGGAGGAGAGCGCTTTCGTGTTAGCTGATAACCTTTTGAAGACCGACATTGTCAGGGAGAATACAGACAATATTCAGTTAGAAGTTGCAAGACTGAGCACGGAAGGAAACCTAGAAGATCTAAAATTCCCAGAGAACATGGGCCACGGAAGTACCATCCAGCTCTCAGCAAACACATTAAAGCAAAATGGCCGGAATGGAGAGATTAGAGTGGCTTTTGTCCTGTATAATAACCTGGGTCCTTATTTGTCTACGGAGAATGCCAGTATGAAGTTGGGCACAGAAGCTATGTCCACAAATCACTCGGTTATCGTCAATTCCCCTGTTATTACAGCAGCAATAAATAAGGAATTCAGTAATAAAGTGTATTTGGCTGATCCTGTGGTATTTACTGTTAAACACATCAAGCAGTCAGAAGAAAATTTCAACCCTAACTGTTCATTTTGGAGCTATTCCAAGCGCACAATGACAGGTTATTGGTCAACACAAGGCTGTCGACTCCTGACAACCAACAAGACACATACTACATGCTCCTGTAACCACCTCACCAACTTTGCAGTATTGATGGCACATGTGGAAGTTAAGCACAGCGATGCAGTCCACGATCTTCTTCTGGATGTGATCACGTGGGTTGGCATCTTGCTGTCCCTTGTCTGTCTCCTGATTTGCATCTTCACATTCTGCTTCTTCCGGGGACTCCAGAGTGACCGCAACACCATTCACAAGAACCTCTGCATCAGCCTGTTCGTGGCAGAACTGCTCTTCCTGATTGGAATCAACAGAACCGACCAACCAATTGCCTGTGCAGTGTTCGCGGCTCTTTTGCATTTCTTCTTCTTGGCGGCCTTCACCTGGATGTTTCTAGAAGGGGTGCAGCTGTATATCATGCTGGTGGAGGTCTTTGAGAGCGAGCATTCCCGTAGGAAGTACTTTTATCTGGTCGGCTATGGGATGCCCGCGCTCATCGTGGCCGTTTCTGCGGCCGTCGACTACAGGAGCTACGGAACAGACAAAGTATGTTGGCTTCGCCTTGACACCTACTTCATTTGGAGTTTTATAGGACCGGCGACCTTGATAATTATGCTGAATGTCATCTTCCTTGGGATTGCTTTATACAAAATGTTTCACCATACTGCCATACTGAAACCTGAATCAGGCTGTCTTGACAATATCAAGTCATGGGTTATAGGTGCAATAGCTCTGCTCTGCCTGTTAGGATTGACCTGGGCCTTTGGACTCATGTATATTAATGAAAGCACAGTCATCATGGCGTATCTCTTCACCATTTTCAATTCTCTACAGGGAATGTTTATATTCATTTTCCACTGTGTTCTACAGAAGAAGGTACGGAAAGAGTATGGGAAATGCCTCCGCACGCATTGCTGTAGTGGGAAAAGCACGGAGAGTTCGATTGGCTCAGGGAAAACATCTGGTTCTCGAACTCCAGGACGGTACTCCACAGGCTCGCAGAGCCGGATTCGGAGAATGTGGAATGACACTGTCCGAAAGCAGTCAGAGTCGTCCTTCATCACCGGAGACATAAACAGCTCAGCATCGCTCAACAGAGGATATCTTCCCTGCATGCAGGCCTGTGTAACCTATGTAGGCGATGGTGGAGACAGTTAA
Peptide sequence:
MVETVNNLLQPQALNAWRDLTTSDQLRAATMLLDTVEESAFVLADNLLKTDIVRENTDNIQLEVARLSTEGNLEDLKFPENMGHGSTIQLSANTLKQNGRNGEIRVAFVLYNNLGPYLSTENASMKLGTEAMSTNHSVIVNSPVITAAINKEFSNKVYLADPVVFTVKHIKQSEENFNPNCSFWSYSKRTMTGYWSTQGCRLLTTNKTHTTCSCNHLTNFAVLMAHVEVKHSDAVHDLLLDVITWVGILLSLVCLLICIFTFCFFRGLQSDRNTIHKNLCISLFVAELLFLIGINRTDQPIACAVFAALLHFFFLAAFTWMFLEGVQLYIMLVEVFESEHSRRKYFYLVGYGMPALIVAVSAAVDYRSYGTDKVCWLRLDTYFIWSFIGPATLIIMLNVIFLGIALYKMFHHTAILKPESGCLDNIKSWVIGAIALLCLLGLTWAFGLMYINESTVIMAYLFTIFNSLQGMFIFIFHCVLQKKVRKEYGKCLRTHCCSGKSTESSIGSGKTSGSRTPGRYSTGSQSRIRRMWNDTVRKQSESSFITGDINSSASLNRGYLPCMQACVTYVGDGGDS
IRESfinder result
IRESfinder score
0.551848454
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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