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mmu_circAdgrl3_1623
Basic information of circular RNA
PanCircBase ID:
mmu_circAdgrl3_1623
mm39_Chr. Position ID:
chr5|81457717|81796579|+
Gene name:
Adgrl3
Transcript:
ENSMUST00000132375.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
4,5,6,7,8,9,10
Exon/intron offsets:
0,155324,199853,202113,250992,336539,338758
Genomic length:
338862
Exon/intron sizes in nt:
20,91,10,15,80,18,13,00,000
Flanking exon/introns:
chr5:81345478-81457717|chr5:81796579-81808108
Splice length:
1623
Other database IDs:
CIRCpedia: MMU_CIRCpedia_30762
Human Ortholog IDs:
Mature spliced Sequence:
GTGTCTCAAGTGGGATATCAGTGTTTATTTTTGAGGAATTTTTCTTTCTGTTTGTGTTTGGTTTTTAATCATTGATATTGGAGTACTGAAGAGGAGCCAGAAGTCTATTGATTTGGTTTCACTTCAGACCAGCCATTCCTCCGAGAACACAGTCATGTGGCCACCACAGCTACTCATCCTCACGATGCTTTTAGCACCTGTAGTTCATGATGCAATAACAGAACCCAGTGTGCAGTGGTGGCAGGTCCTGATGTATTTCCAGACCCATGTCCGGGAACATATAAATACCTTGAAGTGCAGTATGAATGTGTCCCTTATAAAGTGGAACAAAAAGTTTTTCTTTGTCCTGGACTGCTAAAAGGAGTGTACCAGAGTGAACACTTGTTTGAATCTGACCACCAATCTGGGGCATGGTGCAAGGACCCTCTACAGGCTTCTGACAAGATTTACTATATGCCCTGGACTCCCTACAGAACTGACACCCTGACAGAGTATTCGTCCAAAGATGACTTCATTGCTGGAAGGCCAACAACTACCTACAAGCTCCCTCACAGAGTGGATGGCACTGGATTTGTAGTATACGATGGTGCCCTCTTCTTCAACAAGGAGCGGACAAGGAACATTGTAAAGTTTGATTTGAGGACTAGGATAAAGAGTGGAGAGGCAATCATAGCAAATGCTAATTACCATGACACCTCCCCATACCGATGGGGTGGCAAGTCCGACATAGACTTGGCAGTGGATGAAAATGGATTATGGGTAATCTATGCAACAGAACAGAACAATGGCAAGATTGTCATTAGCCAGTTGAACCCTTACACCCTACGGATTGAGGGGACATGGGACACTGCCTATGATAAAAGGTCAGCTTCCAATGCATTCATGATTTGTGGGATTCTGTATGTGGTCAAATCTGTATATGAGGATGATGACAACGAGGCCACCGGTAATAAGATTGACTACATTTACAACACTGACCAAAGCAAGGATAGCTTGGTGGATGTACCCTTTCCCAACTCTTACCAGTACATAGCAGCTGTGGATTACAATCCCAGGGACAATCTGCTCTATGTGTGGAATAACTACCACGTTGTGAAATACTCTTTGGACTTCGGGCCTCTGGATAGTAGATCAGGGCCGGTACATCATGGACAAGTCTCCTACATCTCTCCACCAATTCACCTCGACTCTGAACTAGAAAGGCCCCCTGTCAGAGGGATTTCTACCACAGGATCCCTGGGTATGGGAAGCACGACCACCAGCACCACCCTCCGGACCACAACCTGGAACATAGGCAGGAGTACCACCGCATCCTTGCCGGGCAGAAGAAACCGCAGTACCAGCACGCCATCCCCCGCGGTAGAGGTGCTGGATGACGTCACCACACACCTGCCCTCGGCAGCCTCCCAAATCCCAGCTATGGAAGAGAGCTGCGAGGCTGTGGAAGCCCGAGAAATCATGTGGTTTAAGACCAGACAGGGGCAGGTAGCAAAGCAGCCATGCCCAGCAGGAACCATAGGTGTATCAACTTACCTATGTCTTGCTCCTGATGGAATATGGGACCCCCAAGGACCAGATCTCAGCAACTGCTCTTCTCCTTGGGTCAATCACATAACACAGAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCCAAGGACCAGATCTCAGC
59.82
TGGCTCCTCTTCAGTACTCCA
59.92
158
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CCCCCAAGGACCAGATCTCA
60.62
TGGCTCCTCTTCAGTACTCCA
59.92
160
PCR template sequence:
ATCAACTTACCTATGTCTTGCTCCTGATGGAATATGGGACCCCCAAGGACCAGATCTCAGCAACTGCTCTTCTCCTTGGGTCAATCACATAACACAGAAGGTGTCTCAAGTGGGATATCAGTGTTTATTTTTGAGGAATTTTTCTTTCTGTTTGTGTTTGGTTTTTAATCATTGATATTGGAGTACTGAAGAGGAGCCAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
11
Interacting miRNAs (#binding sites)
mmu-miR-9-5p(1), mmu-miR-34a-5p(1), mmu-miR-33-5p(1), mmu-miR-138-5p(1), mmu-miR-181b-5p(1), mmu-miR-149-5p(1), mmu-miR-370-3p(1), mmu-miR-1198-5p(1), mmu-miR-335-3p(1), mmu-miR-3470b(1), mmu-miR-7a-2-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CATAACACAGAAGGTGTCTCAAGTGG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCADGRL3_1623_ORF_1
0.8426
0.156040617
0.997185825
1245
ORF sequence:
ATGCCCTGGACTCCCTACAGAACTGACACCCTGACAGAGTATTCGTCCAAAGATGACTTCATTGCTGGAAGGCCAACAACTACCTACAAGCTCCCTCACAGAGTGGATGGCACTGGATTTGTAGTATACGATGGTGCCCTCTTCTTCAACAAGGAGCGGACAAGGAACATTGTAAAGTTTGATTTGAGGACTAGGATAAAGAGTGGAGAGGCAATCATAGCAAATGCTAATTACCATGACACCTCCCCATACCGATGGGGTGGCAAGTCCGACATAGACTTGGCAGTGGATGAAAATGGATTATGGGTAATCTATGCAACAGAACAGAACAATGGCAAGATTGTCATTAGCCAGTTGAACCCTTACACCCTACGGATTGAGGGGACATGGGACACTGCCTATGATAAAAGGTCAGCTTCCAATGCATTCATGATTTGTGGGATTCTGTATGTGGTCAAATCTGTATATGAGGATGATGACAACGAGGCCACCGGTAATAAGATTGACTACATTTACAACACTGACCAAAGCAAGGATAGCTTGGTGGATGTACCCTTTCCCAACTCTTACCAGTACATAGCAGCTGTGGATTACAATCCCAGGGACAATCTGCTCTATGTGTGGAATAACTACCACGTTGTGAAATACTCTTTGGACTTCGGGCCTCTGGATAGTAGATCAGGGCCGGTACATCATGGACAAGTCTCCTACATCTCTCCACCAATTCACCTCGACTCTGAACTAGAAAGGCCCCCTGTCAGAGGGATTTCTACCACAGGATCCCTGGGTATGGGAAGCACGACCACCAGCACCACCCTCCGGACCACAACCTGGAACATAGGCAGGAGTACCACCGCATCCTTGCCGGGCAGAAGAAACCGCAGTACCAGCACGCCATCCCCCGCGGTAGAGGTGCTGGATGACGTCACCACACACCTGCCCTCGGCAGCCTCCCAAATCCCAGCTATGGAAGAGAGCTGCGAGGCTGTGGAAGCCCGAGAAATCATGTGGTTTAAGACCAGACAGGGGCAGGTAGCAAAGCAGCCATGCCCAGCAGGAACCATAGGTGTATCAACTTACCTATGTCTTGCTCCTGATGGAATATGGGACCCCCAAGGACCAGATCTCAGCAACTGCTCTTCTCCTTGGGTCAATCACATAACACAGAAGGTGTCTCAAGTGGGATATCAGTGTTTATTTTTGAGGAATTTTTCTTTCTGTTTGTGTTTGGTTTTTAATCATTGA
Peptide sequence:
MPWTPYRTDTLTEYSSKDDFIAGRPTTTYKLPHRVDGTGFVVYDGALFFNKERTRNIVKFDLRTRIKSGEAIIANANYHDTSPYRWGGKSDIDLAVDENGLWVIYATEQNNGKIVISQLNPYTLRIEGTWDTAYDKRSASNAFMICGILYVVKSVYEDDDNEATGNKIDYIYNTDQSKDSLVDVPFPNSYQYIAAVDYNPRDNLLYVWNNYHVVKYSLDFGPLDSRSGPVHHGQVSYISPPIHLDSELERPPVRGISTTGSLGMGSTTTSTTLRTTTWNIGRSTTASLPGRRNRSTSTPSPAVEVLDDVTTHLPSAASQIPAMEESCEAVEAREIMWFKTRQGQVAKQPCPAGTIGVSTYLCLAPDGIWDPQGPDLSNCSSPWVNHITQKVSQVGYQCLFLRNFSFCLCLVFNH
IRESfinder result
IRESfinder score
0.596956178
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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