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mmu_circAdgrl2_387
Basic information of circular RNA
PanCircBase ID:
mmu_circAdgrl2_387
mm39_Chr. Position ID:
chr3|148596047|148660371|-
Gene name:
Adgrl2
Transcript:
ENSMUST00000196526.5
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
3,2
Exon/intron offsets:
0,64151
Genomic length:
64324
Exon/intron sizes in nt:
2,14,173
Flanking exon/introns:
chr3:148571345-148596047|chr3:148660371-148696075
Splice length:
387
Other database IDs:
circAtlas: mmu-Lphn2_0005
circBase: hsa_circ_0013048, circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_32984
Human Ortholog IDs:
Mature spliced Sequence:
ATATGAGGATCAATGATGCAGACTGCTGGGTTCGATGAAGCTGGGCATTTATAACTAGATTCATTAAGGAATACAAAGAAAATATTTAAAGGGATCAATAATGGTGTCTTCTGGCTGCAGAATGCGAAGTCTGTGGTTTATCATTATAATCAGTTTCTCACCGAGTACAGAAGGTTTCAGCAGAGCAGCCTTACCATTCGGGTTAGTTAGACGAGAGCTATCCTGTGAGGGTTATTCTATAGACCTGCGATGTCCGGGCAGTGACGTCATCATGATCGAGAGTGCGAACTACGGTCGGACGGACGACAAGATCTGCGATGCAGACCCCTTTCAAATGGAGAACACAGACTGCTACCTCCCTGATGCCTTCAAAATCATGACTCAAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CGGACGGACGACAAGATCTG
60.52
ATGCCCAGCTTCATCGAACC
60.75
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCGGACGGACGACAAGATCT
60.68
ATGCCCAGCTTCATCGAACC
60.75
141
PCR template sequence:
ACTACGGTCGGACGGACGACAAGATCTGCGATGCAGACCCCTTTCAAATGGAGAACACAGACTGCTACCTCCCTGATGCCTTCAAAATCATGACTCAAAGATATGAGGATCAATGATGCAGACTGCTGGGTTCGATGAAGCTGGGCATTTATAACTAGATTCATTAAGGAATACAAAGAAAATATTTAAAGGGATCAATA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
10
Interacting miRNAs (#binding sites)
mmu-let-7a-5p(1), mmu-let-7e-5p(1), mmu-let-7b-5p(1), mmu-let-7c-5p(1), mmu-let-7d-5p(1), mmu-miR-136-5p(1), mmu-miR-433-3p(1), mmu-miR-199b-3p(1), mmu-miR-199a-3p(1), mmu-miR-98-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TCATGACTCAAAGATATGAGGATCAA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCADGRL2_387_ORF_1
0.6129
0.198632268
0.245491862
303
ORF sequence:
ATGGTGTCTTCTGGCTGCAGAATGCGAAGTCTGTGGTTTATCATTATAATCAGTTTCTCACCGAGTACAGAAGGTTTCAGCAGAGCAGCCTTACCATTCGGGTTAGTTAGACGAGAGCTATCCTGTGAGGGTTATTCTATAGACCTGCGATGTCCGGGCAGTGACGTCATCATGATCGAGAGTGCGAACTACGGTCGGACGGACGACAAGATCTGCGATGCAGACCCCTTTCAAATGGAGAACACAGACTGCTACCTCCCTGATGCCTTCAAAATCATGACTCAAAGATATGAGGATCAATGA
Peptide sequence:
MVSSGCRMRSLWFIIIISFSPSTEGFSRAALPFGLVRRELSCEGYSIDLRCPGSDVIMIESANYGRTDDKICDADPFQMENTDCYLPDAFKIMTQRYEDQ
IRESfinder result
IRESfinder score
0.419665859
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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