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mmu_circAdgrf5_429
Basic information of circular RNA
PanCircBase ID:
mmu_circAdgrf5_429
mm39_Chr. Position ID:
chr17|43750841|43752893|+
Gene name:
Adgrf5
Transcript:
ENSMUST00000226087.2
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
12,13
Exon/intron offsets:
0,1833
Genomic length:
2052
Exon/intron sizes in nt:
210,219
Flanking exon/introns:
chr17:43748577-43750841|chr17:43752893-43755877
Splice length:
429
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
CCAACCTAACAATAACCCGGGACCCAATTTCTGTTTCTGAGGGACAAAGCTTTTCTATAACCTGCCTCAGCGATGTGAGTAGCTTTGATGAGGTGTACTGGAACACCTCAGCTGGTATTAAAATCCACCCAAGGTTTTATACCATGAGGAGGTATCAGGATGGAGCAGAGTCGGTGCTGATGGTGAAGACCTCCACCCGAGAGTGGAACGGAACCTACCACTGCATATTTAGATATAAGAATTCATACAGCATTGCCACCAAAGATGTCACCGTTCACCCGCTGCCTCTGGTGTCAGACATCATGATGGATCCTTTGGAAGCCAGTGGTCTGTGTACCAGTTCCCATCAATTCAAATGCTGCGTAGAAGAGGATGCAGGGGAAGAGTACGCTGTGACTTTCCACGTGGACTCCTCATCCTTTCCCGCTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCTGCGTAGAAGAGGATGCA
60.18
CACATCGCTGAGGCAGGTTA
60.11
149
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGCTGCGTAGAAGAGGATGC
60.18
CACATCGCTGAGGCAGGTTA
60.11
150
PCR template sequence:
CTGTGTACCAGTTCCCATCAATTCAAATGCTGCGTAGAAGAGGATGCAGGGGAAGAGTACGCTGTGACTTTCCACGTGGACTCCTCATCCTTTCCCGCTGCCAACCTAACAATAACCCGGGACCCAATTTCTGTTTCTGAGGGACAAAGCTTTTCTATAACCTGCCTCAGCGATGTGAGTAGCTTTGATGAGGTGTACTG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-9-5p(1), mmu-miR-709(1), mmu-miR-224-5p(1), mmu-miR-673-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TCCTTTCCCGCTGCCAACCTAACAAT
siRNA sets
TAGGTTGGCAGCGGGAAAG, TTAGGTTGGCAGCGGGAAA, AGGTTGGCAGCGGGAAAGG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCADGRF5_429_ORF_1
0.9984
0.23735344
0.937986655
714
ORF sequence:
ATGAGGAGGTATCAGGATGGAGCAGAGTCGGTGCTGATGGTGAAGACCTCCACCCGAGAGTGGAACGGAACCTACCACTGCATATTTAGATATAAGAATTCATACAGCATTGCCACCAAAGATGTCACCGTTCACCCGCTGCCTCTGGTGTCAGACATCATGATGGATCCTTTGGAAGCCAGTGGTCTGTGTACCAGTTCCCATCAATTCAAATGCTGCGTAGAAGAGGATGCAGGGGAAGAGTACGCTGTGACTTTCCACGTGGACTCCTCATCCTTTCCCGCTGCCAACCTAACAATAACCCGGGACCCAATTTCTGTTTCTGAGGGACAAAGCTTTTCTATAACCTGCCTCAGCGATGTGAGTAGCTTTGATGAGGTGTACTGGAACACCTCAGCTGGTATTAAAATCCACCCAAGGTTTTATACCATGAGGAGGTATCAGGATGGAGCAGAGTCGGTGCTGATGGTGAAGACCTCCACCCGAGAGTGGAACGGAACCTACCACTGCATATTTAGATATAAGAATTCATACAGCATTGCCACCAAAGATGTCACCGTTCACCCGCTGCCTCTGGTGTCAGACATCATGATGGATCCTTTGGAAGCCAGTGGTCTGTGTACCAGTTCCCATCAATTCAAATGCTGCGTAGAAGAGGATGCAGGGGAAGAGTACGCTGTGACTTTCCACGTGGACTCCTCATCCTTTCCCGCT
Peptide sequence:
MRRYQDGAESVLMVKTSTREWNGTYHCIFRYKNSYSIATKDVTVHPLPLVSDIMMDPLEASGLCTSSHQFKCCVEEDAGEEYAVTFHVDSSSFPAANLTITRDPISVSEGQSFSITCLSDVSSFDEVYWNTSAGIKIHPRFYTMRRYQDGAESVLMVKTSTREWNGTYHCIFRYKNSYSIATKDVTVHPLPLVSDIMMDPLEASGLCTSSHQFKCCVEEDAGEEYAVTFHVDSSSFPA
IRESfinder result
IRESfinder score
0.594094973
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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