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mmu_circAdgrb3_494
Basic information of circular RNA
PanCircBase ID:
mmu_circAdgrb3_494
mm39_Chr. Position ID:
chr1|25150772|25167969|-
Gene name:
Adgrb3
Transcript:
ENSMUST00000126626.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
10,9,8,7,6
Exon/intron offsets:
0,485,5597,14949,17127
Genomic length:
17197
Exon/intron sizes in nt:
172,99,86,67,70
Flanking exon/introns:
chr1:25140609-25150772|chr1:25167969-25170331
Splice length:
494
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GGCTGCCAGCTTTAGTAGTGGCCACATCTGTAGGCTTCACTAGGACGAAAGGATACGGCACGGATCACTACTGCTGGCTGTCTCTAGAAGGAGGACTGCTCTATGCCTTCGTTGGACCTGCAGCGGCTGTTGTCCTGGTCAACATGGTGATTGGCATTTTGGTATTTAATAAACTTGTTTCCAGAGATGGGATCCTAGATAAAAAGCTAAAACACAGAGCCGGTCAGATGAGTGAGCCTCATAGCGGTCTGACGCTCAAATGTGCCAAGTGTGGAGTAGTTTCTACAACAGCTTTGTCAGCCACCACCGCCAGTAACGCCATGGCCTCTCTTTGGAGCTCCTGTGTGGTGCTACCGCTTCTGGCTCTGACATGGATGTCTGCAGTTCTGGCCATGACAGATAAACGCTCCATATTGTTTCAGATACTTTTTGCCGTGTTTGATTCATTACAAGGCTTTGTGATAGTCATGGTCCACTGCATTCTTCGGAGAGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGTTTCAGATACTTTTTGCCGTGT
59.66
GTGATCCGTGCCGTATCCTT
59.9
148
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGTTTCAGATACTTTTTGCCGTGT
59.66
GTGCCGTATCCTTTCGTCCT
59.82
141
PCR template sequence:
GACAGATAAACGCTCCATATTGTTTCAGATACTTTTTGCCGTGTTTGATTCATTACAAGGCTTTGTGATAGTCATGGTCCACTGCATTCTTCGGAGAGAGGGCTGCCAGCTTTAGTAGTGGCCACATCTGTAGGCTTCACTAGGACGAAAGGATACGGCACGGATCACTACTGCTGGCTGTCTCTAGAAGGAGGACTGCT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-320-3p(1), mmu-miR-149-5p(1), mmu-miR-350-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TCTTCGGAGAGAGGGCTGCCAGCTTT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCADGRB3_494_ORF_1
0.9462
0.018560247
0.312265182
366
ORF sequence:
ATGGTGATTGGCATTTTGGTATTTAATAAACTTGTTTCCAGAGATGGGATCCTAGATAAAAAGCTAAAACACAGAGCCGGTCAGATGAGTGAGCCTCATAGCGGTCTGACGCTCAAATGTGCCAAGTGTGGAGTAGTTTCTACAACAGCTTTGTCAGCCACCACCGCCAGTAACGCCATGGCCTCTCTTTGGAGCTCCTGTGTGGTGCTACCGCTTCTGGCTCTGACATGGATGTCTGCAGTTCTGGCCATGACAGATAAACGCTCCATATTGTTTCAGATACTTTTTGCCGTGTTTGATTCATTACAAGGCTTTGTGATAGTCATGGTCCACTGCATTCTTCGGAGAGAGGGCTGCCAGCTTTAG
Peptide sequence:
MVIGILVFNKLVSRDGILDKKLKHRAGQMSEPHSGLTLKCAKCGVVSTTALSATTASNAMASLWSSCVVLPLLALTWMSAVLAMTDKRSILFQILFAVFDSLQGFVIVMVHCILRREGCQL
IRESfinder result
IRESfinder score
0.43529705
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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