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mmu_circAdgrb3_1450
Basic information of circular RNA
PanCircBase ID:
mmu_circAdgrb3_1450
mm39_Chr. Position ID:
chr1|25435516|25586661|-
Gene name:
Adgrb3
Transcript:
ENSMUST00000146592.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
12
Exon/intron indexes:
15,14,13,12,11,10,9,8,7,6,5,4
Exon/intron offsets:
0,24122,34987,35991,60556,64307,91489,107997,108477,135434,136039,150980
Genomic length:
151145
Exon/intron sizes in nt:
44,10,37,61,50,10,96,90,00,00,00,00,00,00,00,000
Flanking exon/introns:
chr1:25267628-25435516|chr1:25586661-25592755
Splice length:
1450
Other database IDs:
circAtlas: mmu-Bai3_0032
circAtlas: hsa-SCAF8_0001
Human Ortholog IDs:
Mature spliced Sequence:
TACATGGAGTTTGGGAGGAATGGTCACCTTGGAGCTTATGCTCCTTCACATGTGGCCGAGGCCAAAGAACACGAACCAGGTCATGCACACCTCCTCAGTATGGAGGAAGGCCCTGTGAGGGACCTGAAACTCATCACAAGCCTTGTAATATTGCCCTCTGTCCAGTTGACGGACAGTGGCAGGAATGGAGTTCATGGAGCCACTGCTCAGTGACATGTTCTAATGGGACACAGCAGAGGAGTCGGCAGTGCACAGCAGCTGCTCATGGAGGCTCTGAGTGCCGAGGACCATGGGCTGAAAGCAGGGAGTGCTACAACCCTGAATGTACAGCCAATGGTCAGTGGAATCAGTGGGGTCACTGGAGTGGATGTTCCAAGTCCTGTGATGGTGGCTGGGAGAGGAGAATGAGGACTTGTCAGGGCGCAGCTGTAACAGGACAGCAGTGTGAAGGCACAGGTGAAGAGGTACGAAGATGCAGTGAGCAGCGGTGCCCAGCACCTTATGAAATATGCCCAGAGGATTATCTTATATCAATGGTGTGGAAAAGGACTCCTGCAGGCGACCTGGCATTCAATCAATGTCCACTGAATGCCACAGGCACCACTAGCAGACGCTGCTCTCTCAGTCTTCATGGAGTGGCCTCCTGGGAACAGCCGAGCTTTGCAAGATGCATATCCAATGAGTACAGACACTTGCAGCATTCAATTAAAGAGCACCTTGCGAAGGGGCAGCGGATGCTGGCAGGTGATGGAATGTCCCAGGTCACCAAGACACTATTGGATTTAACCCAGAGGAAAAATTTCTATGCAGGTGACCTTCTGGTGTCTGTGGAAATCCTGAGAAATGTGACAGACACGTTTAAAAGGGCAAGTTACATCCCTGCGTCTGATGGGGTCCAGAACTTCTTTCAAATAGTTAGCAACCTTCTTGATGAAGAAAACAAGGAGAAATGGGAAGACGCACAACAGATTTACCCAGGCTCAATAGAGTTAATGCAGGTGATTGAAGATTTTATACACATTGTTGGAATGGGGATGATGGACTTTCAAAATTCATACCTAATGACTGGAAATGTAGTGGCGAGCATTCAGAAGCTCCCTGCTGCATCTGTTCTAACCGACATCAACTTCCCCATGAAAGGCCGGAAAGGAATGGTAGACTGGGCAAGAAATTCAGAAGATCGCGTTGTGATTCCAAAAAGCATTTTCACTCCCGTGTCTTCAAAAGAATTAGATGAATCATCTGTTTTTGTTCTTGGAGCGGTCCTATACAAAAACTTAGACCTAATTTTGCCTACTTTGAGAAATTACACTGTCGTCAATTCTAAAGTCATCGTGGTCACAATCAGGCCTGAACCTAAAACAACTGACTCATTTTTGGAGATAGAGCTAGCTCACTTGGCAAATGGCACTTTGAATCCATATTGCGTGTTATGGGATGACTCAAAATC
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGAGCTAGCTCACTTGGCAA
59.02
TGGTTCGTGTTCTTTGGCCT
60.11
144
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGAGCTAGCTCACTTGGCAA
59.02
TGCATGACCTGGTTCGTGTT
60.18
153
PCR template sequence:
CTGAACCTAAAACAACTGACTCATTTTTGGAGATAGAGCTAGCTCACTTGGCAAATGGCACTTTGAATCCATATTGCGTGTTATGGGATGACTCAAAATCTACATGGAGTTTGGGAGGAATGGTCACCTTGGAGCTTATGCTCCTTCACATGTGGCCGAGGCCAAAGAACACGAACCAGGTCATGCACACCTCCTCAGTA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
14
Interacting miRNAs (#binding sites)
mmu-miR-23b-3p(1), mmu-miR-20b-5p(1), mmu-miR-106a-5p(1), mmu-miR-93-5p(1), mmu-miR-20a-5p(1), mmu-miR-181a-5p(1), mmu-miR-150-5p(1), mmu-miR-335-5p(1), mmu-miR-23a-3p(1), mmu-miR-181b-5p(1), mmu-miR-149-5p(1), mmu-miR-136-5p(1), mmu-miR-338-3p(1), mmu-miR-181d-5p(1)
siRNAs for circular RNA silencing
Junction sequence
ATGACTCAAAATCTACATGGAGTTTG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCADGRB3_1450_ORF_1
0.5399
0.034190769
0.998300897
1461
ORF sequence:
ATGACTCAAAATCTACATGGAGTTTGGGAGGAATGGTCACCTTGGAGCTTATGCTCCTTCACATGTGGCCGAGGCCAAAGAACACGAACCAGGTCATGCACACCTCCTCAGTATGGAGGAAGGCCCTGTGAGGGACCTGAAACTCATCACAAGCCTTGTAATATTGCCCTCTGTCCAGTTGACGGACAGTGGCAGGAATGGAGTTCATGGAGCCACTGCTCAGTGACATGTTCTAATGGGACACAGCAGAGGAGTCGGCAGTGCACAGCAGCTGCTCATGGAGGCTCTGAGTGCCGAGGACCATGGGCTGAAAGCAGGGAGTGCTACAACCCTGAATGTACAGCCAATGGTCAGTGGAATCAGTGGGGTCACTGGAGTGGATGTTCCAAGTCCTGTGATGGTGGCTGGGAGAGGAGAATGAGGACTTGTCAGGGCGCAGCTGTAACAGGACAGCAGTGTGAAGGCACAGGTGAAGAGGTACGAAGATGCAGTGAGCAGCGGTGCCCAGCACCTTATGAAATATGCCCAGAGGATTATCTTATATCAATGGTGTGGAAAAGGACTCCTGCAGGCGACCTGGCATTCAATCAATGTCCACTGAATGCCACAGGCACCACTAGCAGACGCTGCTCTCTCAGTCTTCATGGAGTGGCCTCCTGGGAACAGCCGAGCTTTGCAAGATGCATATCCAATGAGTACAGACACTTGCAGCATTCAATTAAAGAGCACCTTGCGAAGGGGCAGCGGATGCTGGCAGGTGATGGAATGTCCCAGGTCACCAAGACACTATTGGATTTAACCCAGAGGAAAAATTTCTATGCAGGTGACCTTCTGGTGTCTGTGGAAATCCTGAGAAATGTGACAGACACGTTTAAAAGGGCAAGTTACATCCCTGCGTCTGATGGGGTCCAGAACTTCTTTCAAATAGTTAGCAACCTTCTTGATGAAGAAAACAAGGAGAAATGGGAAGACGCACAACAGATTTACCCAGGCTCAATAGAGTTAATGCAGGTGATTGAAGATTTTATACACATTGTTGGAATGGGGATGATGGACTTTCAAAATTCATACCTAATGACTGGAAATGTAGTGGCGAGCATTCAGAAGCTCCCTGCTGCATCTGTTCTAACCGACATCAACTTCCCCATGAAAGGCCGGAAAGGAATGGTAGACTGGGCAAGAAATTCAGAAGATCGCGTTGTGATTCCAAAAAGCATTTTCACTCCCGTGTCTTCAAAAGAATTAGATGAATCATCTGTTTTTGTTCTTGGAGCGGTCCTATACAAAAACTTAGACCTAATTTTGCCTACTTTGAGAAATTACACTGTCGTCAATTCTAAAGTCATCGTGGTCACAATCAGGCCTGAACCTAAAACAACTGACTCATTTTTGGAGATAGAGCTAGCTCACTTGGCAAATGGCACTTTGAATCCATATTGCGTGTTATGGGATGACTCAAAA
Peptide sequence:
MTQNLHGVWEEWSPWSLCSFTCGRGQRTRTRSCTPPQYGGRPCEGPETHHKPCNIALCPVDGQWQEWSSWSHCSVTCSNGTQQRSRQCTAAAHGGSECRGPWAESRECYNPECTANGQWNQWGHWSGCSKSCDGGWERRMRTCQGAAVTGQQCEGTGEEVRRCSEQRCPAPYEICPEDYLISMVWKRTPAGDLAFNQCPLNATGTTSRRCSLSLHGVASWEQPSFARCISNEYRHLQHSIKEHLAKGQRMLAGDGMSQVTKTLLDLTQRKNFYAGDLLVSVEILRNVTDTFKRASYIPASDGVQNFFQIVSNLLDEENKEKWEDAQQIYPGSIELMQVIEDFIHIVGMGMMDFQNSYLMTGNVVASIQKLPAASVLTDINFPMKGRKGMVDWARNSEDRVVIPKSIFTPVSSKELDESSVFVLGAVLYKNLDLILPTLRNYTVVNSKVIVVTIRPEPKTTDSFLEIELAHLANGTLNPYCVLWDDSK
IRESfinder result
IRESfinder score
0.547160551
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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