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mmu_circAdgra3_663
Basic information of circular RNA
PanCircBase ID:
mmu_circAdgra3_663
mm39_Chr. Position ID:
chr5|50164090|50183120|-
Gene name:
Adgra3
Transcript:
ENSMUST00000030971.7
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
6
Exon/intron indexes:
7,6,5,4,3,2
Exon/intron offsets:
0,2587,4742,6644,10078,18958
Genomic length:
19030
Exon/intron sizes in nt:
214,161,72,72,72,72
Flanking exon/introns:
chr5:50159361-50164090|chr5:50183120-50215893
Splice length:
663
Other database IDs:
CIRCpedia: MMU_CIRCpedia_21717, circAtlas: mmu-Gpr125_0004
circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_48230
Human Ortholog IDs:
Mature spliced Sequence:
GATTTTAAGCAACAACAAGATCTCCGAGCTGAAGAATGGTTCATTTTCTGGCTTAAGTCTCCTCGAAAGACTGGACCTCCGGAACAACCTTATTAGTAGGATAGCCCCAGGTGCCTTTTGGGGACTGTCTTCACTGAAGAGATTGGACCTGACGAACAACCGAATAGGTTGTCTGAATGCAGATGTATTTCGAGGACTCACCAATCTGGTTCGGCTAAACCTTTCAGGGAATCTGTTTACTTCACTGTCTCAAGGAACTTTTGATTATCTTGGCTCGTTGCGGTCTTTAGAATTTCAGACTGAGTACCTTCTGTGTGACTGTAACATTCTGTGGATGCATCGCTGGGTAAAGGAGAGAAACATCACTGTGCGAGACACCAGGTGTGTTTATCCCAAGTCACTGCAGGCCCAGCCTGTCACGGGGGTGAAACAGGAGCTCCTCACTTGCGATCCTCCCCTTGAACTGCCGTCCTTCTACATGACTCCGTCGCACCGCCAGGTTGTGTTTGAAGGAGACAGCCTTCCCTTCCAGTGCATGGCTTCCTATATAGATCAGGACATGCAAGTGCTGTGGTATCAGGATGGGCGCATTGTTGAGACCGATGAGTCCCAAGGGATCTTTGTGGAGAAAAGCATGATTCACAATTGCTCCTTGATCGCCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GATGGGCGCATTGTTGAGAC
59.9
CGGAGGTCCAGTCTTTCGAG
59.83
164
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GCGCATTGTTGAGACCGATG
60.25
CGGAGGTCCAGTCTTTCGAG
59.83
159
PCR template sequence:
AAGTGCTGTGGTATCAGGATGGGCGCATTGTTGAGACCGATGAGTCCCAAGGGATCTTTGTGGAGAAAAGCATGATTCACAATTGCTCCTTGATCGCCAGGATTTTAAGCAACAACAAGATCTCCGAGCTGAAGAATGGTTCATTTTCTGGCTTAAGTCTCCTCGAAAGACTGGACCTCCGGAACAACCTTATTAGTAGG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
7
Interacting miRNAs (#binding sites)
mmu-miR-375-3p(1), mmu-miR-34c-5p(1), mmu-miR-30e-5p(1), mmu-miR-30a-5p(1), mmu-miR-30d-5p(1), mmu-miR-34b-5p(1), mmu-miR-377-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CCTTGATCGCCAGGATTTTAAGCAAC
siRNA sets
TTAAAATCCTGGCGATCAA, TAAAATCCTGGCGATCAAG, CTTAAAATCCTGGCGATCA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCADGRA3_663_ORF_1
0.9721
0.196969475
0.988828307
990
ORF sequence:
ATGCATCGCTGGGTAAAGGAGAGAAACATCACTGTGCGAGACACCAGGTGTGTTTATCCCAAGTCACTGCAGGCCCAGCCTGTCACGGGGGTGAAACAGGAGCTCCTCACTTGCGATCCTCCCCTTGAACTGCCGTCCTTCTACATGACTCCGTCGCACCGCCAGGTTGTGTTTGAAGGAGACAGCCTTCCCTTCCAGTGCATGGCTTCCTATATAGATCAGGACATGCAAGTGCTGTGGTATCAGGATGGGCGCATTGTTGAGACCGATGAGTCCCAAGGGATCTTTGTGGAGAAAAGCATGATTCACAATTGCTCCTTGATCGCCAGGATTTTAAGCAACAACAAGATCTCCGAGCTGAAGAATGGTTCATTTTCTGGCTTAAGTCTCCTCGAAAGACTGGACCTCCGGAACAACCTTATTAGTAGGATAGCCCCAGGTGCCTTTTGGGGACTGTCTTCACTGAAGAGATTGGACCTGACGAACAACCGAATAGGTTGTCTGAATGCAGATGTATTTCGAGGACTCACCAATCTGGTTCGGCTAAACCTTTCAGGGAATCTGTTTACTTCACTGTCTCAAGGAACTTTTGATTATCTTGGCTCGTTGCGGTCTTTAGAATTTCAGACTGAGTACCTTCTGTGTGACTGTAACATTCTGTGGATGCATCGCTGGGTAAAGGAGAGAAACATCACTGTGCGAGACACCAGGTGTGTTTATCCCAAGTCACTGCAGGCCCAGCCTGTCACGGGGGTGAAACAGGAGCTCCTCACTTGCGATCCTCCCCTTGAACTGCCGTCCTTCTACATGACTCCGTCGCACCGCCAGGTTGTGTTTGAAGGAGACAGCCTTCCCTTCCAGTGCATGGCTTCCTATATAGATCAGGACATGCAAGTGCTGTGGTATCAGGATGGGCGCATTGTTGAGACCGATGAGTCCCAAGGGATCTTTGTGGAGAAAAGCATGATTCACAATTGCTCCTTGATCGCC
Peptide sequence:
MHRWVKERNITVRDTRCVYPKSLQAQPVTGVKQELLTCDPPLELPSFYMTPSHRQVVFEGDSLPFQCMASYIDQDMQVLWYQDGRIVETDESQGIFVEKSMIHNCSLIARILSNNKISELKNGSFSGLSLLERLDLRNNLISRIAPGAFWGLSSLKRLDLTNNRIGCLNADVFRGLTNLVRLNLSGNLFTSLSQGTFDYLGSLRSLEFQTEYLLCDCNILWMHRWVKERNITVRDTRCVYPKSLQAQPVTGVKQELLTCDPPLELPSFYMTPSHRQVVFEGDSLPFQCMASYIDQDMQVLWYQDGRIVETDESQGIFVEKSMIHNCSLIA
IRESfinder result
IRESfinder score
0.677358208
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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