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mmu_circAdd3_809
Basic information of circular RNA
PanCircBase ID:
mmu_circAdd3_809
mm39_Chr. Position ID:
chr19|53219610|53223392|+
Gene name:
Add3
Transcript:
ENSMUST00000111741.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
6
Exon/intron indexes:
4,5,6,7,8,9
Exon/intron offsets:
0,944,1846,2393,2645,3599
Genomic length:
3782
Exon/intron sizes in nt:
152,81,150,144,99,183
Flanking exon/introns:
chr19:53215160-53219610|chr19:53223392-53225024
Splice length:
809
Other database IDs:
circAtlas: mmu-Add3_0004
circAtlas: hsa-ADD3_0007, CIRCpedia: HSA_CIRCpedia_362
Human Ortholog IDs:
Mature spliced Sequence:
GTCTTGGCATGGTCACACCTATTAATGACCTTCCTGGTGCAGATACATCCTCGTATGTGAAGGGAGAAAAGCTTACTCGCTGCAAACTTGCCAGCCTCTACAGGCTTGCAGACTTGTTTGGGTGGGCGCACCTGGCAAATACGTACATCTCGGTACGAATAAGTAAGGAGCAAGACCACATTATAATAATTCCCAGAGGCCTCTCTTTTTCTGAAGCGACAGCCTCCACTTTGGTGAAAGTCAACATAATCGGAGAAGTGGTGGACCAGGGGAGCACTGATCTGAAAATCGACCACACGGGCTTCAGCCCGCATGCTGCCATCTATTCAACACGCCCCGATGTGAAGTGTGTGATCCACATCCACACCCTTGCCACAGCAGCGGTGTCCTCCATGAAGTGTGGGATCCTTCCAATTTCCCAAGAGTCTCTCATCCTGGGAGATGTCGCCTACTATGACTACCAGGGGTCGCTTGATGAGGAGGAAGAAAGAATTGAACTTCAGAAAGTGCTGGGTCCAAGCTGTAAGGTGCTCGTCCTCAGGAACCATGGCATGGTGGCTCTTGGCGAGACCCTCGAGGAGGCTTTCCATTACATCTTTAATGTACAGATGGCCTGTGAGATCCAGGTGCAGGCAGTAGCAGGGGCAGGCGGAGTAGACAATCTGCTCGTACTGGATCTTCAGAAATATAAAGCTTTCACCCACGGTGTCGCCATGTCTGGAGGAGGCGGTGTAAACATGGCTTCCCATCAGAAGTGGAAGGTTGGCGAGATTGAGTTTGAAGGGCTGATGAGGACCCTGGACAACTTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGGAGGCGGTGTAAACATGG
60.04
CGAGGATGTATCTGCACCAGG
60.27
141
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GAAGTGGAAGGTTGGCGAGA
59.97
GCAGCGAGTAAGCTTTTCTCC
59.6
141
PCR template sequence:
CGCCATGTCTGGAGGAGGCGGTGTAAACATGGCTTCCCATCAGAAGTGGAAGGTTGGCGAGATTGAGTTTGAAGGGCTGATGAGGACCCTGGACAACTTGGTCTTGGCATGGTCACACCTATTAATGACCTTCCTGGTGCAGATACATCCTCGTATGTGAAGGGAGAAAAGCTTACTCGCTGCAAACTTGCCAGCCTCTA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-382-5p(1), mmu-miR-188-3p(1), mmu-miR-7a-2-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CCTGGACAACTTGGTCTTGGCATGGT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCADD3_809_ORF_1
1.1817
0.242549859
0.984039738
861
ORF sequence:
ATGGTCACACCTATTAATGACCTTCCTGGTGCAGATACATCCTCGTATGTGAAGGGAGAAAAGCTTACTCGCTGCAAACTTGCCAGCCTCTACAGGCTTGCAGACTTGTTTGGGTGGGCGCACCTGGCAAATACGTACATCTCGGTACGAATAAGTAAGGAGCAAGACCACATTATAATAATTCCCAGAGGCCTCTCTTTTTCTGAAGCGACAGCCTCCACTTTGGTGAAAGTCAACATAATCGGAGAAGTGGTGGACCAGGGGAGCACTGATCTGAAAATCGACCACACGGGCTTCAGCCCGCATGCTGCCATCTATTCAACACGCCCCGATGTGAAGTGTGTGATCCACATCCACACCCTTGCCACAGCAGCGGTGTCCTCCATGAAGTGTGGGATCCTTCCAATTTCCCAAGAGTCTCTCATCCTGGGAGATGTCGCCTACTATGACTACCAGGGGTCGCTTGATGAGGAGGAAGAAAGAATTGAACTTCAGAAAGTGCTGGGTCCAAGCTGTAAGGTGCTCGTCCTCAGGAACCATGGCATGGTGGCTCTTGGCGAGACCCTCGAGGAGGCTTTCCATTACATCTTTAATGTACAGATGGCCTGTGAGATCCAGGTGCAGGCAGTAGCAGGGGCAGGCGGAGTAGACAATCTGCTCGTACTGGATCTTCAGAAATATAAAGCTTTCACCCACGGTGTCGCCATGTCTGGAGGAGGCGGTGTAAACATGGCTTCCCATCAGAAGTGGAAGGTTGGCGAGATTGAGTTTGAAGGGCTGATGAGGACCCTGGACAACTTGGTCTTGGCATGGTCACACCTATTAATGACCTTCCTGGTGCAGATACATCCTCGTATGTGA
Peptide sequence:
MVTPINDLPGADTSSYVKGEKLTRCKLASLYRLADLFGWAHLANTYISVRISKEQDHIIIIPRGLSFSEATASTLVKVNIIGEVVDQGSTDLKIDHTGFSPHAAIYSTRPDVKCVIHIHTLATAAVSSMKCGILPISQESLILGDVAYYDYQGSLDEEEERIELQKVLGPSCKVLVLRNHGMVALGETLEEAFHYIFNVQMACEIQVQAVAGAGGVDNLLVLDLQKYKAFTHGVAMSGGGGVNMASHQKWKVGEIEFEGLMRTLDNLVLAWSHLLMTFLVQIHPRM
IRESfinder result
IRESfinder score
0.423188472
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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