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mmu_circAdd1_700
Basic information of circular RNA
PanCircBase ID:
mmu_circAdd1_700
mm39_Chr. Position ID:
chr5|34773906|34785857|+
Gene name:
Add1
Transcript:
ENSMUST00000114338.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
10,11,12,13,14
Exon/intron offsets:
0,2787,3482,8716,11852
Genomic length:
11951
Exon/intron sizes in nt:
252,102,90,157,99
Flanking exon/introns:
chr5:34771704-34773906|chr5:34785857-34786479
Splice length:
700
Other database IDs:
circAtlas: hsa-ADD1_0043, CIRCpedia: HSA_CIRCpedia_137124
Human Ortholog IDs:
Mature spliced Sequence:
GGCTACAGAACTGGCTACCCTTATCGATACCCTGCTCTGAGAGAGAGATCTAAAAAGTACAGCGATGTGGAAGTCCCTGCCAGTGTCACAGGCCACTCCTTTGCTAGTGATGGCGATTCGGGCACTTGCTCGCCGCTCAGGCACAGTTTTCAGAAGCAGCAGCGAGAGAAGACAAGATGGCTGCACTCTGGCCGGGGTGATGACGCTTCTGAGGAGGGGCAGAACGGAAGCAGTCCCAAGTCGAAGACTAAGTGGACTAAAGAGGATGGACATAGAACTTCCACCTCTGCTGTCCCTAACCTGTTTGTTCCATTGAACACTAACCCAAAAGAGGTCCAGGAGATGAGGAACAAGATTCGAGAGCAGAACTTACAGGACATTAAGACGGCAGGACCTCAGTCCCAGGTTTTGTGTGGTGTGATGATGGACAGAAGCCTTGTTCAGGGGGAGCTGGTGACGGCCTCCAAAGCCATCATTGAGAAGGAATACCAGCCCCATGTAATTGTGAGCACCACAGGTCCCAACCCCTTCAACACGCTGACAGACCGTGAACTGGAAGAATACCGACGAGAGGTGGAGCGGAAGCAGAAGGGCTCTGAAGAGAACCTGGATGAGACAAGAGAGCAGAAAGAGAAGAGTCCTCCAGACCAGTCAGCTGTCCCCAACACTCCTCCCAGTACTCCTGTCAAGCTTGAAGAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CTCCAGACCAGTCAGCTGTC
59.75
CTGGCAGGGACTTCCACATC
60.39
143
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CTCCAGACCAGTCAGCTGTC
59.75
GTGACACTGGCAGGGACTTC
60.6
149
PCR template sequence:
GAGAACCTGGATGAGACAAGAGAGCAGAAAGAGAAGAGTCCTCCAGACCAGTCAGCTGTCCCCAACACTCCTCCCAGTACTCCTGTCAAGCTTGAAGAAGGGCTACAGAACTGGCTACCCTTATCGATACCCTGCTCTGAGAGAGAGATCTAAAAAGTACAGCGATGTGGAAGTCCCTGCCAGTGTCACAGGCCACTCCT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-1904(2), mmu-miR-298-5p(1), mmu-miR-495-3p(1)
siRNAs for circular RNA silencing
Junction sequence
AAGCTTGAAGAAGGGCTACAGAACTG
siRNA sets
AGTTCTGTAGCCCTTCTTC, GTTCTGTAGCCCTTCTTCA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCADD1_700_ORF_1
0.9187
0.338516453
0.970980334
789
ORF sequence:
ATGAGACAAGAGAGCAGAAAGAGAAGAGTCCTCCAGACCAGTCAGCTGTCCCCAACACTCCTCCCAGTACTCCTGTCAAGCTTGAAGAAGGGCTACAGAACTGGCTACCCTTATCGATACCCTGCTCTGAGAGAGAGATCTAAAAAGTACAGCGATGTGGAAGTCCCTGCCAGTGTCACAGGCCACTCCTTTGCTAGTGATGGCGATTCGGGCACTTGCTCGCCGCTCAGGCACAGTTTTCAGAAGCAGCAGCGAGAGAAGACAAGATGGCTGCACTCTGGCCGGGGTGATGACGCTTCTGAGGAGGGGCAGAACGGAAGCAGTCCCAAGTCGAAGACTAAGTGGACTAAAGAGGATGGACATAGAACTTCCACCTCTGCTGTCCCTAACCTGTTTGTTCCATTGAACACTAACCCAAAAGAGGTCCAGGAGATGAGGAACAAGATTCGAGAGCAGAACTTACAGGACATTAAGACGGCAGGACCTCAGTCCCAGGTTTTGTGTGGTGTGATGATGGACAGAAGCCTTGTTCAGGGGGAGCTGGTGACGGCCTCCAAAGCCATCATTGAGAAGGAATACCAGCCCCATGTAATTGTGAGCACCACAGGTCCCAACCCCTTCAACACGCTGACAGACCGTGAACTGGAAGAATACCGACGAGAGGTGGAGCGGAAGCAGAAGGGCTCTGAAGAGAACCTGGATGAGACAAGAGAGCAGAAAGAGAAGAGTCCTCCAGACCAGTCAGCTGTCCCCAACACTCCTCCCAGTACTCCTGTCAAGCTTGAAGAA
Peptide sequence:
MRQESRKRRVLQTSQLSPTLLPVLLSSLKKGYRTGYPYRYPALRERSKKYSDVEVPASVTGHSFASDGDSGTCSPLRHSFQKQQREKTRWLHSGRGDDASEEGQNGSSPKSKTKWTKEDGHRTSTSAVPNLFVPLNTNPKEVQEMRNKIREQNLQDIKTAGPQSQVLCGVMMDRSLVQGELVTASKAIIEKEYQPHVIVSTTGPNPFNTLTDRELEEYRREVERKQKGSEENLDETREQKEKSPPDQSAVPNTPPSTPVKLEE
IRESfinder result
IRESfinder score
0.384619203
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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