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mmu_circAdcy9_426
Basic information of circular RNA
PanCircBase ID:
mmu_circAdcy9_426
mm39_Chr. Position ID:
chr16|4125019|4130653|-
Gene name:
Adcy9
Transcript:
ENSMUST00000117801.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
6,5,4
Exon/intron offsets:
0,4369,5529
Genomic length:
5634
Exon/intron sizes in nt:
10,32,18,105
Flanking exon/introns:
chr16:4122449-4125019|chr16:4130653-4141630
Splice length:
426
Other database IDs:
CIRCpedia: MMU_CIRCpedia_226610, circAtlas: mmu-Adcy9_0009
circBase: hsa_circ_0105407, circAtlas: hsa-ADCY9_0005, CIRCpedia: HSA_CIRCpedia_17578
Human Ortholog IDs:
Mature spliced Sequence:
ACTTGCCCTTCTTGTGGAATCACATTTGCTCCCAAATCTGAAGCTGGTGCAGAAGGAGGAACTGTGCAAAATGGCTGTCAAGACGAGCCTAAGACCAGCACCAAGGCTTCTGGAGGACCCAACTCCAAAACCCAGAATGGACTTCTGAGCCCTCCTGCAGAGGAGAAGCTCACTAACAGCCAGACCTCCCTCTGTGAGATCCTGCAAGAGAAGGGACGGTGGGCAGGGGTGAGCTTGGACCAGTCAGCCCTCCTCCCGCTCAGGTTCAAGAACATCCGTGAGAAAACTGATGCCCACTTTGTTGATGTCATCAAAGAAGACAGCCTGATGAAAGATTATTTCTTCAAGCCGCCCATCAATCAGTTCAGCCTGAACTTCCTGGACCAGGAGCTGGAGCGATCATATAGAACCAGCTACCAGGAAGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCTTCAAGCCGCCCATCAAT
60.03
CCTTCTGCACCAGCTTCAGA
59.96
142
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCTTCAAGCCGCCCATCAAT
60.03
TCCTTCTGCACCAGCTTCAG
59.96
143
PCR template sequence:
GATGAAAGATTATTTCTTCAAGCCGCCCATCAATCAGTTCAGCCTGAACTTCCTGGACCAGGAGCTGGAGCGATCATATAGAACCAGCTACCAGGAAGAGACTTGCCCTTCTTGTGGAATCACATTTGCTCCCAAATCTGAAGCTGGTGCAGAAGGAGGAACTGTGCAAAATGGCTGTCAAGACGAGCCTAAGACCAGCA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-29b-1-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CTACCAGGAAGAGACTTGCCCTTCTT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCADCY9_426_ORF_1
0.9309
0.386478861
0.862570821
525
ORF sequence:
ATGAAAGATTATTTCTTCAAGCCGCCCATCAATCAGTTCAGCCTGAACTTCCTGGACCAGGAGCTGGAGCGATCATATAGAACCAGCTACCAGGAAGAGACTTGCCCTTCTTGTGGAATCACATTTGCTCCCAAATCTGAAGCTGGTGCAGAAGGAGGAACTGTGCAAAATGGCTGTCAAGACGAGCCTAAGACCAGCACCAAGGCTTCTGGAGGACCCAACTCCAAAACCCAGAATGGACTTCTGAGCCCTCCTGCAGAGGAGAAGCTCACTAACAGCCAGACCTCCCTCTGTGAGATCCTGCAAGAGAAGGGACGGTGGGCAGGGGTGAGCTTGGACCAGTCAGCCCTCCTCCCGCTCAGGTTCAAGAACATCCGTGAGAAAACTGATGCCCACTTTGTTGATGTCATCAAAGAAGACAGCCTGATGAAAGATTATTTCTTCAAGCCGCCCATCAATCAGTTCAGCCTGAACTTCCTGGACCAGGAGCTGGAGCGATCATATAGAACCAGCTACCAGGAAGAG
Peptide sequence:
MKDYFFKPPINQFSLNFLDQELERSYRTSYQEETCPSCGITFAPKSEAGAEGGTVQNGCQDEPKTSTKASGGPNSKTQNGLLSPPAEEKLTNSQTSLCEILQEKGRWAGVSLDQSALLPLRFKNIREKTDAHFVDVIKEDSLMKDYFFKPPINQFSLNFLDQELERSYRTSYQEE
IRESfinder result
IRESfinder score
0.453734155
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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