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mmu_circAdcy7_318
Basic information of circular RNA
PanCircBase ID:
mmu_circAdcy7_318
mm39_Chr. Position ID:
chr8|89036319|89037311|+
Gene name:
Adcy7
Transcript:
ENSMUST00000169037.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
6,7
Exon/intron offsets:
0,842
Genomic length:
992
Exon/intron sizes in nt:
168,150
Flanking exon/introns:
chr8:89035472-89036319|chr8:89037311-89037626
Splice length:
318
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTGCCTTTCTTCCTGTTTGTCGTCTTTGTGGTGTATGCACTACTGCCTCTCAGCAGGAGGGCAGCCATCGTGGCAGGCGTGACCTCCACGGTCTCCCATCTCCTGGTGTTTGGAGCTGTGACAAGAGCCTTCCAGACGTCCATGTCTAGCACTCAACTGGGGCTGCAGCTCCTGGCCAATGCCGTTATCCTCCTGGGTGGGAACTTCACGGGTGCCTTCCACAAGCACCAGCTGCAGGACGCGTCCAGGGATCTCTTTATCTACACCGTCAAATGCATCCAGATCCGTCGGAAGCTTCGTGTGGAGAAGCGCCAGCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CGCGTCCAGGGATCTCTTTA
59.26
CTGCCCTCCTGCTGAGAG
59.1
143
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CGCGTCCAGGGATCTCTTTA
59.26
GCTGCCCTCCTGCTGAGAG
62.39
144
PCR template sequence:
CCACAAGCACCAGCTGCAGGACGCGTCCAGGGATCTCTTTATCTACACCGTCAAATGCATCCAGATCCGTCGGAAGCTTCGTGTGGAGAAGCGCCAGCAGGTGCCTTTCTTCCTGTTTGTCGTCTTTGTGGTGTATGCACTACTGCCTCTCAGCAGGAGGGCAGCCATCGTGGCAGGCGTGACCTCCACGGTCTCCCATC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
10
Interacting miRNAs (#binding sites)
mmu-miR-124-3p(1), mmu-let-7a-5p(1), mmu-miR-138-5p(1), mmu-let-7d-5p(1), mmu-miR-676-3p(1), mmu-miR-127-5p(1), mmu-miR-199b-3p(1), mmu-miR-199a-3p(1), mmu-miR-370-3p(1), mmu-miR-673-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GAAGCGCCAGCAGGTGCCTTTCTTCC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCADCY7_318_ORF_1
1.3032
0.480175965
0.937075345
495
ORF sequence:
ATGTCTAGCACTCAACTGGGGCTGCAGCTCCTGGCCAATGCCGTTATCCTCCTGGGTGGGAACTTCACGGGTGCCTTCCACAAGCACCAGCTGCAGGACGCGTCCAGGGATCTCTTTATCTACACCGTCAAATGCATCCAGATCCGTCGGAAGCTTCGTGTGGAGAAGCGCCAGCAGGTGCCTTTCTTCCTGTTTGTCGTCTTTGTGGTGTATGCACTACTGCCTCTCAGCAGGAGGGCAGCCATCGTGGCAGGCGTGACCTCCACGGTCTCCCATCTCCTGGTGTTTGGAGCTGTGACAAGAGCCTTCCAGACGTCCATGTCTAGCACTCAACTGGGGCTGCAGCTCCTGGCCAATGCCGTTATCCTCCTGGGTGGGAACTTCACGGGTGCCTTCCACAAGCACCAGCTGCAGGACGCGTCCAGGGATCTCTTTATCTACACCGTCAAATGCATCCAGATCCGTCGGAAGCTTCGTGTGGAGAAGCGCCAGCAG
Peptide sequence:
MSSTQLGLQLLANAVILLGGNFTGAFHKHQLQDASRDLFIYTVKCIQIRRKLRVEKRQQVPFFLFVVFVVYALLPLSRRAAIVAGVTSTVSHLLVFGAVTRAFQTSMSSTQLGLQLLANAVILLGGNFTGAFHKHQLQDASRDLFIYTVKCIQIRRKLRVEKRQQ
IRESfinder result
IRESfinder score
0.510855262
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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