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mmu_circAdcy5_682
Basic information of circular RNA
PanCircBase ID:
mmu_circAdcy5_682
mm39_Chr. Position ID:
chr16|35087549|35094935|+
Gene name:
Adcy5
Transcript:
ENSMUST00000114913.3
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
4,5,6,7,8
Exon/intron offsets:
0,1776,3658,4899,7245
Genomic length:
7386
Exon/intron sizes in nt:
11,21,28,15,91,42,141
Flanking exon/introns:
chr16:35073632-35087549|chr16:35094935-35098864
Splice length:
682
Other database IDs:
CIRCpedia: MMU_CIRCpedia_17677, circAtlas: mmu-Adcy5_0001
circBase: hsa_circ_0121670, circAtlas: hsa-ADCY5_0002, CIRCpedia: HSA_CIRCpedia_138657
Human Ortholog IDs:
Mature spliced Sequence:
CATCCTGTTTGCTGACATCGAGGGCTTCACCAGCCTGGCTTCCCAGTGTACTGCCCAAGAACTGGTCATGACCCTCAATGAGCTCTTCGCCCGCTTTGACAAGTTGGCTGCGGAGAATCACTGTTTACGGATTAAGATCCTCGGGGATTGTTACTACTGCGTCTCGGGGCTGCCTGAAGCCAGAGCCGACCATGCCCACTGCTGCGTGGAGATGGGAATGGACATGATCGAGGCCATCTCGTTGGTCCGGGAGGTGACAGGGGTGAACGTGAACATGCGCGTGGGAATTCACAGCGGGAGAGTACACTGCGGTGTCCTTGGTCTCAGAAAGTGGCAATTCGACGTGTGGTCTAACGATGTCACTCTGGCCAACCACATGGAAGCTGGTGGCAAGGCGGGCCGCATCCACATCACCAAGGCCACACTCAACTACCTGAATGGGGACTATGAGGTGGAACCAGGCTGTGGTGGCGATCGCAATGCCTACCTCAAGGAGCACAGCATTGAGACCTTCCTCATCCTGAGCTGTACCCAGAAGCGGAAAGAAGAGAAGGCCATGATCGCCAAGATGAACCGCCAGAGAACCAACTCCATCGGACACAATCCGCCTCACTGGGGAGCCGAGCGCCCCTTCTACAACCACTTGGGCGGCAACCAGGTGTCAAAGGAGATGAAGAGGATG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CGCCCCTTCTACAACCACTT
59.96
GCGAAGAGCTCATTGAGGGT
60.11
147
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TTCTACAACCACTTGGGCGG
60.25
GCGAAGAGCTCATTGAGGGT
60.11
141
PCR template sequence:
AACCAACTCCATCGGACACAATCCGCCTCACTGGGGAGCCGAGCGCCCCTTCTACAACCACTTGGGCGGCAACCAGGTGTCAAAGGAGATGAAGAGGATGCATCCTGTTTGCTGACATCGAGGGCTTCACCAGCCTGGCTTCCCAGTGTACTGCCCAAGAACTGGTCATGACCCTCAATGAGCTCTTCGCCCGCTTTGAC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
6
Interacting miRNAs (#binding sites)
mmu-let-7e-5p(1), mmu-miR-196a-5p(1), mmu-miR-339-5p(1), mmu-miR-29b-1-5p(1), mmu-miR-98-5p(1), mmu-miR-574-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GATGAAGAGGATGCATCCTGTTTGCT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCADCY5_682_ORF_1
1.0534
0.470364903
0.959040391
630
ORF sequence:
ATGACCCTCAATGAGCTCTTCGCCCGCTTTGACAAGTTGGCTGCGGAGAATCACTGTTTACGGATTAAGATCCTCGGGGATTGTTACTACTGCGTCTCGGGGCTGCCTGAAGCCAGAGCCGACCATGCCCACTGCTGCGTGGAGATGGGAATGGACATGATCGAGGCCATCTCGTTGGTCCGGGAGGTGACAGGGGTGAACGTGAACATGCGCGTGGGAATTCACAGCGGGAGAGTACACTGCGGTGTCCTTGGTCTCAGAAAGTGGCAATTCGACGTGTGGTCTAACGATGTCACTCTGGCCAACCACATGGAAGCTGGTGGCAAGGCGGGCCGCATCCACATCACCAAGGCCACACTCAACTACCTGAATGGGGACTATGAGGTGGAACCAGGCTGTGGTGGCGATCGCAATGCCTACCTCAAGGAGCACAGCATTGAGACCTTCCTCATCCTGAGCTGTACCCAGAAGCGGAAAGAAGAGAAGGCCATGATCGCCAAGATGAACCGCCAGAGAACCAACTCCATCGGACACAATCCGCCTCACTGGGGAGCCGAGCGCCCCTTCTACAACCACTTGGGCGGCAACCAGGTGTCAAAGGAGATGAAGAGGATGCATCCTGTTTGCTGA
Peptide sequence:
MTLNELFARFDKLAAENHCLRIKILGDCYYCVSGLPEARADHAHCCVEMGMDMIEAISLVREVTGVNVNMRVGIHSGRVHCGVLGLRKWQFDVWSNDVTLANHMEAGGKAGRIHITKATLNYLNGDYEVEPGCGGDRNAYLKEHSIETFLILSCTQKRKEEKAMIAKMNRQRTNSIGHNPPHWGAERPFYNHLGGNQVSKEMKRMHPVC
IRESfinder result
IRESfinder score
0.237233717
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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